Salacia chinensis L. Stem extract exerts antifibrotic effects on human hepatic stellate cells through the inhibition of the tgf-β1-induced smad2/3 signaling pathway

Salacia chinensis L. Stem extract exerts antifibrotic effects on human hepatic stellate cells through the inhibition of the tgf-β1-induced smad2/3 signaling pathway

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Salacia chinensis L. (SC) stems have been used as an ingredient in Thai traditional medicine for treating patients with hepatic fibrosis and liver cirrhosis. However, there is no scientific evidence supporting the antifibrotic effects of SC e...

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Main Authors: Mattareeyapar Phaosri, Salinee Jantrapirom, Mingkwan Na Takuathung, Noppamas Soonthornchareonnon, Seewaboon Sireeratawong, Pensiri Buacheen, Pornsiri Pitchakarn, Wutigri Nimlamool, Saranyapin Potikanond
Format: Journal
Published: 2020
Subjects:
Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Chemistry
Computer Science
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85076830412&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/67594
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Institution: Chiang Mai University
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Summary:© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Salacia chinensis L. (SC) stems have been used as an ingredient in Thai traditional medicine for treating patients with hepatic fibrosis and liver cirrhosis. However, there is no scientific evidence supporting the antifibrotic effects of SC extract. Therefore, this study aimed to determine the antifibrotic activity of SC stem extract in human hepatic stellate cell-line called LX-2. We found that upon TGF-β1 stimulation, LX-2 cells transformed to a myofibroblast-like phenotype with a noticeable increase in α-SMA and collagen type I production. Interestingly, cells treated with SC extract significantly suppressed α-SMA and collagen type I production and reversed the myofibroblast-like characteristics back to normal. Additionally, TGF-β1 also influenced the development of fibrogenesis by upregulation of MMP-2, TIMP-1, and TIMP-2 and related cellular signaling, such as pSmad2/3, pErk1/2, and pJNK. Surprisingly, SC possesses antifibrotic activity through the suppression of TGF-β1-mediated production of collagen type 1, α-SMA, and the phosphorylation status of Smad2/3, Erk1/2, and JNK. Taken together, the present study provides accumulated information demonstrating the antifibrotic effects of SC stem extract and revealing its potential for development for hepatic fibrosis patients.

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