Actinomycetes from tropical limestone caves
The cultivable actinomycetes from tropical limestone caverns at Khao No-Khao Kaeo karst, Thailand, were investigated. In total, 276 actinomycetes were isolated from 10 soil samples using different selective isolation procedures. The predominant actinomycetes from all samples were members of the genu...
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th-cmuir.6653943832-67762014-08-30T03:51:14Z Actinomycetes from tropical limestone caves Niyomvong N. Pathom-Aree W. Thamchaipenet A. Duangmal K. The cultivable actinomycetes from tropical limestone caverns at Khao No-Khao Kaeo karst, Thailand, were investigated. In total, 276 actinomycetes were isolated from 10 soil samples using different selective isolation procedures. The predominant actinomycetes from all samples were members of the genus Streptomyces (94.6%) as they contained LL-diaminopimelic acid (A 2pm) in their whole cell hydrolysates. The remaining 15 isolates (5.4%), rich in meso-A 2pm in whole cell hydrolysates were characterized by 16S rRNA gene sequencing. They were shown to represent strains from 8 different genera namely Actinomadura, Actinoplanes, Gordonia, Microbispora, Micromonospora, Nocardia, Nonomuraea and Saccharopolyspora. A good agreement was found between the results based on morphology, presence of meso-A 2pm and 16S rRNA gene phylogeny. Microwave treatment of soil samples was found to promote the isolation of rare actinomycetes. Successful isolation of members of the genus Actinomadura and Saccharopolyspora from caves was reported for the first time. This study confirms significant diversity of cultivable actinomycetes from neglected habitats such as limestone caves in Thailand. 2014-08-30T03:51:13Z 2014-08-30T03:51:13Z 2012 Article 1252526 http://www.scopus.com/inward/record.url?eid=2-s2.0-84865169791&partnerID=40&md5=e2ca140e9c007e4dc33edd4cf8cdf48d http://cmuir.cmu.ac.th/handle/6653943832/6776 English |
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The cultivable actinomycetes from tropical limestone caverns at Khao No-Khao Kaeo karst, Thailand, were investigated. In total, 276 actinomycetes were isolated from 10 soil samples using different selective isolation procedures. The predominant actinomycetes from all samples were members of the genus Streptomyces (94.6%) as they contained LL-diaminopimelic acid (A 2pm) in their whole cell hydrolysates. The remaining 15 isolates (5.4%), rich in meso-A 2pm in whole cell hydrolysates were characterized by 16S rRNA gene sequencing. They were shown to represent strains from 8 different genera namely Actinomadura, Actinoplanes, Gordonia, Microbispora, Micromonospora, Nocardia, Nonomuraea and Saccharopolyspora. A good agreement was found between the results based on morphology, presence of meso-A 2pm and 16S rRNA gene phylogeny. Microwave treatment of soil samples was found to promote the isolation of rare actinomycetes. Successful isolation of members of the genus Actinomadura and Saccharopolyspora from caves was reported for the first time. This study confirms significant diversity of cultivable actinomycetes from neglected habitats such as limestone caves in Thailand. |
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Article |
author |
Niyomvong N. Pathom-Aree W. Thamchaipenet A. Duangmal K. |
spellingShingle |
Niyomvong N. Pathom-Aree W. Thamchaipenet A. Duangmal K. Actinomycetes from tropical limestone caves |
author_facet |
Niyomvong N. Pathom-Aree W. Thamchaipenet A. Duangmal K. |
author_sort |
Niyomvong N. |
title |
Actinomycetes from tropical limestone caves |
title_short |
Actinomycetes from tropical limestone caves |
title_full |
Actinomycetes from tropical limestone caves |
title_fullStr |
Actinomycetes from tropical limestone caves |
title_full_unstemmed |
Actinomycetes from tropical limestone caves |
title_sort |
actinomycetes from tropical limestone caves |
publishDate |
2014 |
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http://www.scopus.com/inward/record.url?eid=2-s2.0-84865169791&partnerID=40&md5=e2ca140e9c007e4dc33edd4cf8cdf48d http://cmuir.cmu.ac.th/handle/6653943832/6776 |
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