Intrinsic cellular responses of human wharton’s jelly mesenchymal stem cells influenced by O<inf>2</inf>-plasma-modified and unmodified surface of alkaline-hydrolyzed 2D and 3D PCL scaffolds

© 2019 by the authors. Polycaprolactone (PCL), a hydrophobic-degradable polyester, has been widely investigated and extensively developed, to increase the biocompatibility for tissue engineering. This research was the first trial to evaluate the intrinsic biological responses of human Wharton’s Jell...

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Main Authors: Kewalin Inthanon, Wanida Janvikul, Siriwan Ongchai, Siriwadee Chomdej
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/67804
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spelling th-cmuir.6653943832-678042020-04-02T15:08:55Z Intrinsic cellular responses of human wharton’s jelly mesenchymal stem cells influenced by O<inf>2</inf>-plasma-modified and unmodified surface of alkaline-hydrolyzed 2D and 3D PCL scaffolds Kewalin Inthanon Wanida Janvikul Siriwan Ongchai Siriwadee Chomdej Engineering Materials Science © 2019 by the authors. Polycaprolactone (PCL), a hydrophobic-degradable polyester, has been widely investigated and extensively developed, to increase the biocompatibility for tissue engineering. This research was the first trial to evaluate the intrinsic biological responses of human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) cultured on alkaline hydrolysis and low-pressure oxygen plasma modified 2D and 3D PCL scaffolds, without adding any differentiation inducers; this has not been reported before. Four types of the substrate were newly established: 2D plasma-treated PCL (2D-TP), 2D non-plasma-treated PCL (2D-NP), 3D plasma-treated PCL (3D-TP), and 3D non-plasma-treated PCL (3D-NP). Physicochemical characterization revealed that only plasma-treated PCL scaffolds significantly increased the hydrophilicity and % oxygen/carbon ratio on the surfaces. The RMS roughness of 3D was higher than 2D conformation, whilst the plasma-treated surfaces were rougher than the non-plasma treated ones. The cytocompatibility test demonstrated that the 2D PCLs enhanced the initial cell attachment in comparison to the 3Ds, indicated by a higher expression of focal adhesion kinase. Meanwhile, the 3Ds promoted cell proliferation and migration as evidence of higher cyclin-A expression and filopodial protrusion, respectively. The 3Ds potentially protected the cell from apoptosis/necrosis but also altered the pluripotency/differentiation-related gene expression. In summary, the different configuration and surface properties of PCL scaffolds displayed the significant potential and effectiveness for facilitating stem cell growth and differentiation in vitro. The cell–substrate interactions on modified surface PCL may provide some information which could be further applied in substrate architecture for stem cell accommodation in cell delivery system for tissue repair. 2020-04-02T15:04:43Z 2020-04-02T15:04:43Z 2019-12-01 Journal 20794983 2-s2.0-85078756241 10.3390/jfb10040052 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85078756241&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/67804
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Engineering
Materials Science
spellingShingle Engineering
Materials Science
Kewalin Inthanon
Wanida Janvikul
Siriwan Ongchai
Siriwadee Chomdej
Intrinsic cellular responses of human wharton’s jelly mesenchymal stem cells influenced by O<inf>2</inf>-plasma-modified and unmodified surface of alkaline-hydrolyzed 2D and 3D PCL scaffolds
description © 2019 by the authors. Polycaprolactone (PCL), a hydrophobic-degradable polyester, has been widely investigated and extensively developed, to increase the biocompatibility for tissue engineering. This research was the first trial to evaluate the intrinsic biological responses of human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) cultured on alkaline hydrolysis and low-pressure oxygen plasma modified 2D and 3D PCL scaffolds, without adding any differentiation inducers; this has not been reported before. Four types of the substrate were newly established: 2D plasma-treated PCL (2D-TP), 2D non-plasma-treated PCL (2D-NP), 3D plasma-treated PCL (3D-TP), and 3D non-plasma-treated PCL (3D-NP). Physicochemical characterization revealed that only plasma-treated PCL scaffolds significantly increased the hydrophilicity and % oxygen/carbon ratio on the surfaces. The RMS roughness of 3D was higher than 2D conformation, whilst the plasma-treated surfaces were rougher than the non-plasma treated ones. The cytocompatibility test demonstrated that the 2D PCLs enhanced the initial cell attachment in comparison to the 3Ds, indicated by a higher expression of focal adhesion kinase. Meanwhile, the 3Ds promoted cell proliferation and migration as evidence of higher cyclin-A expression and filopodial protrusion, respectively. The 3Ds potentially protected the cell from apoptosis/necrosis but also altered the pluripotency/differentiation-related gene expression. In summary, the different configuration and surface properties of PCL scaffolds displayed the significant potential and effectiveness for facilitating stem cell growth and differentiation in vitro. The cell–substrate interactions on modified surface PCL may provide some information which could be further applied in substrate architecture for stem cell accommodation in cell delivery system for tissue repair.
format Journal
author Kewalin Inthanon
Wanida Janvikul
Siriwan Ongchai
Siriwadee Chomdej
author_facet Kewalin Inthanon
Wanida Janvikul
Siriwan Ongchai
Siriwadee Chomdej
author_sort Kewalin Inthanon
title Intrinsic cellular responses of human wharton’s jelly mesenchymal stem cells influenced by O<inf>2</inf>-plasma-modified and unmodified surface of alkaline-hydrolyzed 2D and 3D PCL scaffolds
title_short Intrinsic cellular responses of human wharton’s jelly mesenchymal stem cells influenced by O<inf>2</inf>-plasma-modified and unmodified surface of alkaline-hydrolyzed 2D and 3D PCL scaffolds
title_full Intrinsic cellular responses of human wharton’s jelly mesenchymal stem cells influenced by O<inf>2</inf>-plasma-modified and unmodified surface of alkaline-hydrolyzed 2D and 3D PCL scaffolds
title_fullStr Intrinsic cellular responses of human wharton’s jelly mesenchymal stem cells influenced by O<inf>2</inf>-plasma-modified and unmodified surface of alkaline-hydrolyzed 2D and 3D PCL scaffolds
title_full_unstemmed Intrinsic cellular responses of human wharton’s jelly mesenchymal stem cells influenced by O<inf>2</inf>-plasma-modified and unmodified surface of alkaline-hydrolyzed 2D and 3D PCL scaffolds
title_sort intrinsic cellular responses of human wharton’s jelly mesenchymal stem cells influenced by o<inf>2</inf>-plasma-modified and unmodified surface of alkaline-hydrolyzed 2d and 3d pcl scaffolds
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85078756241&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/67804
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