Cytotoxic and larvicidal activities of Stemona alkaloids from the aerial parts and roots of Stemona curtisii Hook.f.

© 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group. A new Stemona alkaloid glycoside derivative, 6-hydroxy-5,6-seco-stemocurtisinoside (4), was isolated from the ethanolic extract of the aerial parts of Stemona curtisii Hook.f., together with stemocurtisine (1), (11Z)-1'...

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Main Authors: Sirinrat Chalom, Jukreera Panyakaew, Manlika Phaya, Stephen G. Pyne, Pitchaya Mungkornasawakul
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/68197
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-681972020-04-02T15:24:55Z Cytotoxic and larvicidal activities of Stemona alkaloids from the aerial parts and roots of Stemona curtisii Hook.f. Sirinrat Chalom Jukreera Panyakaew Manlika Phaya Stephen G. Pyne Pitchaya Mungkornasawakul Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Chemistry © 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group. A new Stemona alkaloid glycoside derivative, 6-hydroxy-5,6-seco-stemocurtisinoside (4), was isolated from the ethanolic extract of the aerial parts of Stemona curtisii Hook.f., together with stemocurtisine (1), (11Z)-1',2'-didehydrostemofoline (2) and 6-hydroxy-5,6-seco-stemocurtisine (3). Whereas, stemocurtisine (1), stemocurtisinol (5) and oxyprotostemonine (6) were isolated from the roots. Their structures were elucidated using 1D and 2D NMR spectroscopy as well as MS experiments. The extract and the pure isolated compounds were evaluated for their cytotoxicities and their larvicidal activities against the dengue vector, Aedes aegypti. The alkaloid 2 showed the strongest larvicidal activity with a LC50 value of 2.44 µM. While the alkaloid 3 exhibited cytotoxicity against MCF-7 and KB cells (IC50 values of 62.52 and 18.82 µM, respectively) and showed no significant cytotoxicity against Vero cells. Additionally, quantitative analysis of the most active compounds; 2 and 3 in the crude extracts was also performed by HPLC. 2020-04-02T15:23:17Z 2020-04-02T15:23:17Z 2020-01-01 Journal 14786427 14786419 2-s2.0-85078625684 10.1080/14786419.2019.1709188 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85078625684&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/68197
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Chemistry
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Chemistry
Sirinrat Chalom
Jukreera Panyakaew
Manlika Phaya
Stephen G. Pyne
Pitchaya Mungkornasawakul
Cytotoxic and larvicidal activities of Stemona alkaloids from the aerial parts and roots of Stemona curtisii Hook.f.
description © 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group. A new Stemona alkaloid glycoside derivative, 6-hydroxy-5,6-seco-stemocurtisinoside (4), was isolated from the ethanolic extract of the aerial parts of Stemona curtisii Hook.f., together with stemocurtisine (1), (11Z)-1',2'-didehydrostemofoline (2) and 6-hydroxy-5,6-seco-stemocurtisine (3). Whereas, stemocurtisine (1), stemocurtisinol (5) and oxyprotostemonine (6) were isolated from the roots. Their structures were elucidated using 1D and 2D NMR spectroscopy as well as MS experiments. The extract and the pure isolated compounds were evaluated for their cytotoxicities and their larvicidal activities against the dengue vector, Aedes aegypti. The alkaloid 2 showed the strongest larvicidal activity with a LC50 value of 2.44 µM. While the alkaloid 3 exhibited cytotoxicity against MCF-7 and KB cells (IC50 values of 62.52 and 18.82 µM, respectively) and showed no significant cytotoxicity against Vero cells. Additionally, quantitative analysis of the most active compounds; 2 and 3 in the crude extracts was also performed by HPLC.
format Journal
author Sirinrat Chalom
Jukreera Panyakaew
Manlika Phaya
Stephen G. Pyne
Pitchaya Mungkornasawakul
author_facet Sirinrat Chalom
Jukreera Panyakaew
Manlika Phaya
Stephen G. Pyne
Pitchaya Mungkornasawakul
author_sort Sirinrat Chalom
title Cytotoxic and larvicidal activities of Stemona alkaloids from the aerial parts and roots of Stemona curtisii Hook.f.
title_short Cytotoxic and larvicidal activities of Stemona alkaloids from the aerial parts and roots of Stemona curtisii Hook.f.
title_full Cytotoxic and larvicidal activities of Stemona alkaloids from the aerial parts and roots of Stemona curtisii Hook.f.
title_fullStr Cytotoxic and larvicidal activities of Stemona alkaloids from the aerial parts and roots of Stemona curtisii Hook.f.
title_full_unstemmed Cytotoxic and larvicidal activities of Stemona alkaloids from the aerial parts and roots of Stemona curtisii Hook.f.
title_sort cytotoxic and larvicidal activities of stemona alkaloids from the aerial parts and roots of stemona curtisii hook.f.
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85078625684&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68197
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