Antileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leaves

© 2020 MDPI AG. All rights reserved. Kaffir lime (Citrus hystrix) is a plant member of family Rutaceae, and its leaves are commonly used in folk medicine. The present study explores antileukemic effects of the extracts and purified active compounds from the leaves. The antileukemic activity was inve...

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Main Authors: Songyot Anuchapreeda, Fah Chueahongthong, Natsima Viriyaadhammaa, Pawaret Panyajai, Riki Anzawa, Singkome Tima, Chadarat Ampasavate, Aroonchai Saiai, Methee Rungrojsakul, Toyonobu Usuki, Siriporn Okonogi
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/68232
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-682322020-04-02T15:29:39Z Antileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leaves Songyot Anuchapreeda Fah Chueahongthong Natsima Viriyaadhammaa Pawaret Panyajai Riki Anzawa Singkome Tima Chadarat Ampasavate Aroonchai Saiai Methee Rungrojsakul Toyonobu Usuki Siriporn Okonogi Biochemistry, Genetics and Molecular Biology Chemistry Pharmacology, Toxicology and Pharmaceutics © 2020 MDPI AG. All rights reserved. Kaffir lime (Citrus hystrix) is a plant member of family Rutaceae, and its leaves are commonly used in folk medicine. The present study explores antileukemic effects of the extracts and purified active compounds from the leaves. The antileukemic activity was investigated via inhibition of Wilms' tumor 1 (WT1), which is a protein that involves in leukemic cell proliferation. In addition, the compounds were investigated for their effects on WT1 gene expression using real time RT-PCR and Western blotting. Cell cycle arrest and total cell number were investigated using flow cytometry and trypan blue exclusion method, respectively. The results demonstrated that the hexane fractionated extract had the greatest inhibitory effect on WT1 gene expression of many leukemic cell lines and significantly decreased WT1 protein levels of K562 cells (representative of the leukemic cells), in a dose- and time-dependent manner. Subfraction No. 9 (F9) after partial purification of hexane fractioned extract showed the highest suppression on WT1 protein and suppressed cell cycle at G2/M. The organic compounds were isolated from F9 and identified as phytol and lupeol. The bioassays confirmed antiproliferative activities of natural products phytol and lupeol. The results demonstrated anticancer activity of the isolated phytol and lupeol to decrease leukemic cell proliferation. 2020-04-02T15:23:35Z 2020-04-02T15:23:35Z 2020-03-02 Journal 14203049 2-s2.0-85081686177 10.3390/molecules25061300 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081686177&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/68232
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Pharmacology, Toxicology and Pharmaceutics
Songyot Anuchapreeda
Fah Chueahongthong
Natsima Viriyaadhammaa
Pawaret Panyajai
Riki Anzawa
Singkome Tima
Chadarat Ampasavate
Aroonchai Saiai
Methee Rungrojsakul
Toyonobu Usuki
Siriporn Okonogi
Antileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leaves
description © 2020 MDPI AG. All rights reserved. Kaffir lime (Citrus hystrix) is a plant member of family Rutaceae, and its leaves are commonly used in folk medicine. The present study explores antileukemic effects of the extracts and purified active compounds from the leaves. The antileukemic activity was investigated via inhibition of Wilms' tumor 1 (WT1), which is a protein that involves in leukemic cell proliferation. In addition, the compounds were investigated for their effects on WT1 gene expression using real time RT-PCR and Western blotting. Cell cycle arrest and total cell number were investigated using flow cytometry and trypan blue exclusion method, respectively. The results demonstrated that the hexane fractionated extract had the greatest inhibitory effect on WT1 gene expression of many leukemic cell lines and significantly decreased WT1 protein levels of K562 cells (representative of the leukemic cells), in a dose- and time-dependent manner. Subfraction No. 9 (F9) after partial purification of hexane fractioned extract showed the highest suppression on WT1 protein and suppressed cell cycle at G2/M. The organic compounds were isolated from F9 and identified as phytol and lupeol. The bioassays confirmed antiproliferative activities of natural products phytol and lupeol. The results demonstrated anticancer activity of the isolated phytol and lupeol to decrease leukemic cell proliferation.
format Journal
author Songyot Anuchapreeda
Fah Chueahongthong
Natsima Viriyaadhammaa
Pawaret Panyajai
Riki Anzawa
Singkome Tima
Chadarat Ampasavate
Aroonchai Saiai
Methee Rungrojsakul
Toyonobu Usuki
Siriporn Okonogi
author_facet Songyot Anuchapreeda
Fah Chueahongthong
Natsima Viriyaadhammaa
Pawaret Panyajai
Riki Anzawa
Singkome Tima
Chadarat Ampasavate
Aroonchai Saiai
Methee Rungrojsakul
Toyonobu Usuki
Siriporn Okonogi
author_sort Songyot Anuchapreeda
title Antileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leaves
title_short Antileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leaves
title_full Antileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leaves
title_fullStr Antileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leaves
title_full_unstemmed Antileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leaves
title_sort antileukemic cell proliferation of active compounds from kaffir lime (citrus hystrix) leaves
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081686177&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68232
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