New antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, Streptomyces palmae CMU-AB204<sup>T</sup>

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Basal stem rot (BSR), or Ganoderma rot disease, is the most serious disease associated with the oil palm plant of Southeast Asian countries. A basidiomycetous fungus, Ganoderma boninense, is the causative microbe of this disease. To control B...

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Main Authors: Kanaporn Sujarit, Mihoko Mori, Kazuyuki Dobashi, Kazuro Shiomi, Wasu Pathom-Aree, Saisamorn Lumyong
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/68426
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-684262020-04-02T15:28:09Z New antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, Streptomyces palmae CMU-AB204<sup>T</sup> Kanaporn Sujarit Mihoko Mori Kazuyuki Dobashi Kazuro Shiomi Wasu Pathom-Aree Saisamorn Lumyong Immunology and Microbiology Medicine © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Basal stem rot (BSR), or Ganoderma rot disease, is the most serious disease associated with the oil palm plant of Southeast Asian countries. A basidiomycetous fungus, Ganoderma boninense, is the causative microbe of this disease. To control BSR in oil palm plantations, biological control agents are gaining attention as a major alternative to chemical fungicides. In the course of searching for effective actinomycetes as potential biological control agents for BSR, Streptomyces palmae CMU-AB204T was isolated from oil palm rhizosphere soil collected on the campus of Chiang Mai University. The culture broth of this strain showed significant antimicrobial activities against several bacteria and phytopathogenic fungi including G. boninense. Antifungal and antibacterial compounds were isolated by antimicrobial activity-guided purification using chromatographic methods. Their structures were elucidated by spectroscopic techniques, including Nuclear Magnetic Resonance (NMR), Mass Spectrometry (MS), Ultraviolet (UV), and Infrared (IR) analyses. The current study isolated new phenyl alkenoic acids 1–6 and three known compounds, anguinomycin A (7), leptomycin A (8), and actinopyrone A (9) as antimicrobial agents. Compounds 1 and 2 displayed broad antifungal activity, though they did not show antibacterial activity. Compounds 3 and 4 revealed a strong antibacterial activity against both Gram-positive and Gram-negative bacteria including the phytopathogenic strain Xanthomonas campestris pv. oryzae. Compounds 7–9 displayed antifungal activity against Ganoderma. Thus, the antifungal compounds obtained in this study may play a role in protecting oil palm plants from Ganoderma infection with the strain S. palmae CMU-AB204T. 2020-04-02T15:26:54Z 2020-04-02T15:26:54Z 2020-03-01 Journal 20762607 2-s2.0-85080976291 10.3390/microorganisms8030350 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85080976291&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/68426
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Kanaporn Sujarit
Mihoko Mori
Kazuyuki Dobashi
Kazuro Shiomi
Wasu Pathom-Aree
Saisamorn Lumyong
New antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, Streptomyces palmae CMU-AB204<sup>T</sup>
description © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Basal stem rot (BSR), or Ganoderma rot disease, is the most serious disease associated with the oil palm plant of Southeast Asian countries. A basidiomycetous fungus, Ganoderma boninense, is the causative microbe of this disease. To control BSR in oil palm plantations, biological control agents are gaining attention as a major alternative to chemical fungicides. In the course of searching for effective actinomycetes as potential biological control agents for BSR, Streptomyces palmae CMU-AB204T was isolated from oil palm rhizosphere soil collected on the campus of Chiang Mai University. The culture broth of this strain showed significant antimicrobial activities against several bacteria and phytopathogenic fungi including G. boninense. Antifungal and antibacterial compounds were isolated by antimicrobial activity-guided purification using chromatographic methods. Their structures were elucidated by spectroscopic techniques, including Nuclear Magnetic Resonance (NMR), Mass Spectrometry (MS), Ultraviolet (UV), and Infrared (IR) analyses. The current study isolated new phenyl alkenoic acids 1–6 and three known compounds, anguinomycin A (7), leptomycin A (8), and actinopyrone A (9) as antimicrobial agents. Compounds 1 and 2 displayed broad antifungal activity, though they did not show antibacterial activity. Compounds 3 and 4 revealed a strong antibacterial activity against both Gram-positive and Gram-negative bacteria including the phytopathogenic strain Xanthomonas campestris pv. oryzae. Compounds 7–9 displayed antifungal activity against Ganoderma. Thus, the antifungal compounds obtained in this study may play a role in protecting oil palm plants from Ganoderma infection with the strain S. palmae CMU-AB204T.
format Journal
author Kanaporn Sujarit
Mihoko Mori
Kazuyuki Dobashi
Kazuro Shiomi
Wasu Pathom-Aree
Saisamorn Lumyong
author_facet Kanaporn Sujarit
Mihoko Mori
Kazuyuki Dobashi
Kazuro Shiomi
Wasu Pathom-Aree
Saisamorn Lumyong
author_sort Kanaporn Sujarit
title New antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, Streptomyces palmae CMU-AB204<sup>T</sup>
title_short New antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, Streptomyces palmae CMU-AB204<sup>T</sup>
title_full New antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, Streptomyces palmae CMU-AB204<sup>T</sup>
title_fullStr New antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, Streptomyces palmae CMU-AB204<sup>T</sup>
title_full_unstemmed New antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, Streptomyces palmae CMU-AB204<sup>T</sup>
title_sort new antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, streptomyces palmae cmu-ab204<sup>t</sup>
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85080976291&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68426
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