Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pig Farms in Luzon, Philippines

© Copyright 2020, Mary Ann Liebert, Inc. This cross-sectional study was conducted to determine the prevalence, antibiogram, and resistance profile of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) isolates from healthy pigs and pig farms in Luzon, Philippines. A total of 162 rect...

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Main Authors: Romeo S. Gundran, Paul A. Cardenio, Roderick T. Salvador, Fredelon B. Sison, Carolyn C. Benigno, Kwanchai Kreausukon, Duangporn Pichpol, Veerasak Punyapornwithaya
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Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/68431
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-684312020-04-02T15:29:41Z Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pig Farms in Luzon, Philippines Romeo S. Gundran Paul A. Cardenio Roderick T. Salvador Fredelon B. Sison Carolyn C. Benigno Kwanchai Kreausukon Duangporn Pichpol Veerasak Punyapornwithaya Immunology and Microbiology Medicine Pharmacology, Toxicology and Pharmaceutics © Copyright 2020, Mary Ann Liebert, Inc. This cross-sectional study was conducted to determine the prevalence, antibiogram, and resistance profile of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) isolates from healthy pigs and pig farms in Luzon, Philippines. A total of 162 rectal samples from healthy finisher and breeder pigs and boot swab samples from pig houses were collected from 54 randomly selected pig farms. Bacteria were isolated and screened using MacConkey agar plate supplemented with 1 mg/L cefotaxime. Identification of bacteria and antimicrobial susceptibility test were carried out through Vitek® 2 and combined disk test. PCR amplifications were carried out in all isolates targeting blaCTX-M and its five major groupings, blaTEM, and blaSHV. The farm prevalence of ESBL-EC was 57.41% (95% confidence interval [CI] = 43.21-70.77). A total of 48 (29.63%) ESBL-EC isolates were isolated from samples that showed 14 different phenotypic multidrug resistance patterns. The prevalence of blaCTX-M gene was 91.67% (95% CI = 80.02-97.68). All major blaCTX-M-groups except blaCTX-M-25group were detected. The blaCTX-M-1 was the most prevalent blaCTX-M gene, 75.0% (95% CI = 60.40-86.36). The prevalence of blaTEM and blaSHV genes was 91.67% (95% CI = 80.02-97.68) and 60.42% (95% CI = 45.27-74.23), respectively. Coexistence of different blaCTX-M, blaTEM, and blaSHV genes was observed in 44 isolates with 20 different genotypic patterns. High prevalence, diverse antibiogram profile, and genotypic resistance pattern of ESBL-EC isolates from healthy pigs and pig farms were observed in this study that could result in possible transmission to farm workers, susceptible bacteria, and the environment. 2020-04-02T15:26:56Z 2020-04-02T15:26:56Z 2020-02-01 Journal 19318448 10766294 2-s2.0-85079074862 10.1089/mdr.2019.0019 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85079074862&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/68431
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Immunology and Microbiology
Medicine
Pharmacology, Toxicology and Pharmaceutics
Romeo S. Gundran
Paul A. Cardenio
Roderick T. Salvador
Fredelon B. Sison
Carolyn C. Benigno
Kwanchai Kreausukon
Duangporn Pichpol
Veerasak Punyapornwithaya
Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pig Farms in Luzon, Philippines
description © Copyright 2020, Mary Ann Liebert, Inc. This cross-sectional study was conducted to determine the prevalence, antibiogram, and resistance profile of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) isolates from healthy pigs and pig farms in Luzon, Philippines. A total of 162 rectal samples from healthy finisher and breeder pigs and boot swab samples from pig houses were collected from 54 randomly selected pig farms. Bacteria were isolated and screened using MacConkey agar plate supplemented with 1 mg/L cefotaxime. Identification of bacteria and antimicrobial susceptibility test were carried out through Vitek® 2 and combined disk test. PCR amplifications were carried out in all isolates targeting blaCTX-M and its five major groupings, blaTEM, and blaSHV. The farm prevalence of ESBL-EC was 57.41% (95% confidence interval [CI] = 43.21-70.77). A total of 48 (29.63%) ESBL-EC isolates were isolated from samples that showed 14 different phenotypic multidrug resistance patterns. The prevalence of blaCTX-M gene was 91.67% (95% CI = 80.02-97.68). All major blaCTX-M-groups except blaCTX-M-25group were detected. The blaCTX-M-1 was the most prevalent blaCTX-M gene, 75.0% (95% CI = 60.40-86.36). The prevalence of blaTEM and blaSHV genes was 91.67% (95% CI = 80.02-97.68) and 60.42% (95% CI = 45.27-74.23), respectively. Coexistence of different blaCTX-M, blaTEM, and blaSHV genes was observed in 44 isolates with 20 different genotypic patterns. High prevalence, diverse antibiogram profile, and genotypic resistance pattern of ESBL-EC isolates from healthy pigs and pig farms were observed in this study that could result in possible transmission to farm workers, susceptible bacteria, and the environment.
format Journal
author Romeo S. Gundran
Paul A. Cardenio
Roderick T. Salvador
Fredelon B. Sison
Carolyn C. Benigno
Kwanchai Kreausukon
Duangporn Pichpol
Veerasak Punyapornwithaya
author_facet Romeo S. Gundran
Paul A. Cardenio
Roderick T. Salvador
Fredelon B. Sison
Carolyn C. Benigno
Kwanchai Kreausukon
Duangporn Pichpol
Veerasak Punyapornwithaya
author_sort Romeo S. Gundran
title Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pig Farms in Luzon, Philippines
title_short Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pig Farms in Luzon, Philippines
title_full Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pig Farms in Luzon, Philippines
title_fullStr Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pig Farms in Luzon, Philippines
title_full_unstemmed Prevalence, Antibiogram, and Resistance Profile of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates from Pig Farms in Luzon, Philippines
title_sort prevalence, antibiogram, and resistance profile of extended-spectrum β-lactamase-producing escherichia coli isolates from pig farms in luzon, philippines
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85079074862&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68431
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