Associations between expression levels of O-GlcNAc transferase (OGT) and chemo-response in osteosarcoma

© JOURNAL OF THE MEDICAL ASSOCIATION OF THAILAND Objective: The present study aimed to investigate global levels of O-GlcNAcylation (O-GlcNAc), expressions of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) in osteosarcoma and to explore a prognostic marker. Materials and Methods: The authors studi...

Full description

Saved in:
Bibliographic Details
Main Authors: S. Rattanakuntee, P. Chaiyawat, D. Pruksakorn, S. Kritsanaprakornkit, A. Makeudom, C. Supanchart
Format: Journal
Published: 2020
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85079411225&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68511
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-68511
record_format dspace
spelling th-cmuir.6653943832-685112020-04-02T15:28:39Z Associations between expression levels of O-GlcNAc transferase (OGT) and chemo-response in osteosarcoma S. Rattanakuntee P. Chaiyawat D. Pruksakorn S. Kritsanaprakornkit A. Makeudom C. Supanchart Medicine © JOURNAL OF THE MEDICAL ASSOCIATION OF THAILAND Objective: The present study aimed to investigate global levels of O-GlcNAcylation (O-GlcNAc), expressions of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) in osteosarcoma and to explore a prognostic marker. Materials and Methods: The authors studied in three independent experiments. Western blot analysis was performed to detect the expressions of O-GlcNAcylated proteins, OGT, and OGA in cell lines derived from seven patients with osteosarcoma stage IIB. The band intensities were analyzed using Scion Image software. The correlations between clinicopathologic characters of osteosarcoma cases and expression levels of O-GlcNAcylated proteins, OGT, and OGA were analyzed by using Student’s t-test. Results: The results demonstrated a tendency of higher OGT expression in primary osteosarcoma cells. Furthermore, the level of OGT was elevated in patients who poorly responded to chemotherapy and had shorter survival time (p<0.05). The present study revealed a promising use of OGT as a prognostic marker in osteosarcoma. Conclusion: The present study showed significant higher levels of OGT in osteosarcoma patients who poorly responded to chemotherapy. Furthermore, levels of OGT relate to survival time of osteosarcoma. These findings suggest a role for OGT in chemo-response of osteosarcoma cells. The present study was performed in a limited number of cases, thereby the finding requires further validation in a larger cohort. 2020-04-02T15:28:39Z 2020-04-02T15:28:39Z 2020-01-01 Journal 01252208 2-s2.0-85079411225 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85079411225&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/68511
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
S. Rattanakuntee
P. Chaiyawat
D. Pruksakorn
S. Kritsanaprakornkit
A. Makeudom
C. Supanchart
Associations between expression levels of O-GlcNAc transferase (OGT) and chemo-response in osteosarcoma
description © JOURNAL OF THE MEDICAL ASSOCIATION OF THAILAND Objective: The present study aimed to investigate global levels of O-GlcNAcylation (O-GlcNAc), expressions of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) in osteosarcoma and to explore a prognostic marker. Materials and Methods: The authors studied in three independent experiments. Western blot analysis was performed to detect the expressions of O-GlcNAcylated proteins, OGT, and OGA in cell lines derived from seven patients with osteosarcoma stage IIB. The band intensities were analyzed using Scion Image software. The correlations between clinicopathologic characters of osteosarcoma cases and expression levels of O-GlcNAcylated proteins, OGT, and OGA were analyzed by using Student’s t-test. Results: The results demonstrated a tendency of higher OGT expression in primary osteosarcoma cells. Furthermore, the level of OGT was elevated in patients who poorly responded to chemotherapy and had shorter survival time (p<0.05). The present study revealed a promising use of OGT as a prognostic marker in osteosarcoma. Conclusion: The present study showed significant higher levels of OGT in osteosarcoma patients who poorly responded to chemotherapy. Furthermore, levels of OGT relate to survival time of osteosarcoma. These findings suggest a role for OGT in chemo-response of osteosarcoma cells. The present study was performed in a limited number of cases, thereby the finding requires further validation in a larger cohort.
format Journal
author S. Rattanakuntee
P. Chaiyawat
D. Pruksakorn
S. Kritsanaprakornkit
A. Makeudom
C. Supanchart
author_facet S. Rattanakuntee
P. Chaiyawat
D. Pruksakorn
S. Kritsanaprakornkit
A. Makeudom
C. Supanchart
author_sort S. Rattanakuntee
title Associations between expression levels of O-GlcNAc transferase (OGT) and chemo-response in osteosarcoma
title_short Associations between expression levels of O-GlcNAc transferase (OGT) and chemo-response in osteosarcoma
title_full Associations between expression levels of O-GlcNAc transferase (OGT) and chemo-response in osteosarcoma
title_fullStr Associations between expression levels of O-GlcNAc transferase (OGT) and chemo-response in osteosarcoma
title_full_unstemmed Associations between expression levels of O-GlcNAc transferase (OGT) and chemo-response in osteosarcoma
title_sort associations between expression levels of o-glcnac transferase (ogt) and chemo-response in osteosarcoma
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85079411225&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68511
_version_ 1681426833156866048