Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes
Surface of chitosan membrane was modified with argon (Ar) and nitrogen (N) plasma immersion ion implantation (PIII) for human skin fibroblasts F1544 cell attachment. The modified surfaces were characterized by Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). Cell att...
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th-cmuir.6653943832-68522014-08-30T03:51:19Z Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes Saranwong N. Inthanon K. Wongkham W. Wanichapichart P. Suwannakachorn D. Yu L.D. Surface of chitosan membrane was modified with argon (Ar) and nitrogen (N) plasma immersion ion implantation (PIII) for human skin fibroblasts F1544 cell attachment. The modified surfaces were characterized by Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). Cell attachment patterns were evaluated by scanning electron microscopy (SEM). The enzyme-linked immunosorbent assay (ELISA) was used to quantify levels of focal adhesion kinase (FAK). The results showed that Ar PIII had an enhancement effect on the cell attachment while N-PIII had an inhibition effect. Filopodial analysis revealed more microfilament cytoplasmic spreading on the edge of cells attached on the Ar-treated membranes than N-treated membranes. Higher level FAK was found in Ar-treated membranes than that in N-treated membranes. © 2011 Elsevier B.V. All rights reserved. 2014-08-30T03:51:19Z 2014-08-30T03:51:19Z Article in Press 0168583X 10.1016/j.nimb.2011.01.107 NIMBE http://www.scopus.com/inward/record.url?eid=2-s2.0-79951583978&partnerID=40&md5=4ecf22ed462485138eec89978f2d7afd http://cmuir.cmu.ac.th/handle/6653943832/6852 English |
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Surface of chitosan membrane was modified with argon (Ar) and nitrogen (N) plasma immersion ion implantation (PIII) for human skin fibroblasts F1544 cell attachment. The modified surfaces were characterized by Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). Cell attachment patterns were evaluated by scanning electron microscopy (SEM). The enzyme-linked immunosorbent assay (ELISA) was used to quantify levels of focal adhesion kinase (FAK). The results showed that Ar PIII had an enhancement effect on the cell attachment while N-PIII had an inhibition effect. Filopodial analysis revealed more microfilament cytoplasmic spreading on the edge of cells attached on the Ar-treated membranes than N-treated membranes. Higher level FAK was found in Ar-treated membranes than that in N-treated membranes. © 2011 Elsevier B.V. All rights reserved. |
| format |
Article |
| author |
Saranwong N. Inthanon K. Wongkham W. Wanichapichart P. Suwannakachorn D. Yu L.D. |
| spellingShingle |
Saranwong N. Inthanon K. Wongkham W. Wanichapichart P. Suwannakachorn D. Yu L.D. Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes |
| author_facet |
Saranwong N. Inthanon K. Wongkham W. Wanichapichart P. Suwannakachorn D. Yu L.D. |
| author_sort |
Saranwong N. |
| title |
Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes |
| title_short |
Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes |
| title_full |
Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes |
| title_fullStr |
Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes |
| title_full_unstemmed |
Surface and protein analyses of normal human cell attachment on PIII-modified chitosan membranes |
| title_sort |
surface and protein analyses of normal human cell attachment on piii-modified chitosan membranes |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-79951583978&partnerID=40&md5=4ecf22ed462485138eec89978f2d7afd http://cmuir.cmu.ac.th/handle/6653943832/6852 |
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1681420691398721536 |