Lactobacillus plantarum L-137 and/or β-glucan impacted the histopathological, antioxidant, immune-related genes and resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila

© 2020 Elsevier Ltd A trial was operated to assess the potential of using Lactobacillus plantarum L-137 (L-137) and/or β-glucan (BG) in improving the resistance of Nile tilapia against Aeromonas hydrophila. Control diet and 3 diets supplemented with L-137, BG or L-137 + BG were prepared. Final body...

Full description

Saved in:
Bibliographic Details
Main Authors: Mahmoud A.O. Dawood, Eman Moustafa Moustafa, Zizy I. Elbialy, Foad Farrag, Emad E.E. Lolo, Hanaa A. Abdel-Daim, Mohamed M. Abdel-Daim, Hien Van Doan
Format: Journal
Published: 2020
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081938814&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68592
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-68592
record_format dspace
spelling th-cmuir.6653943832-685922020-04-02T15:30:40Z Lactobacillus plantarum L-137 and/or β-glucan impacted the histopathological, antioxidant, immune-related genes and resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila Mahmoud A.O. Dawood Eman Moustafa Moustafa Zizy I. Elbialy Foad Farrag Emad E.E. Lolo Hanaa A. Abdel-Daim Mohamed M. Abdel-Daim Hien Van Doan Veterinary © 2020 Elsevier Ltd A trial was operated to assess the potential of using Lactobacillus plantarum L-137 (L-137) and/or β-glucan (BG) in improving the resistance of Nile tilapia against Aeromonas hydrophila. Control diet and 3 diets supplemented with L-137, BG or L-137 + BG were prepared. Final body weight, specific growth rate, superoxide dismutase, and catalase showed considerably (P < .05) increased values in L-137 or L-137/BG groups, while glutathione peroxidase increased significantly (P < .05) only in L-137/BG group. Fish fed L-137 and/or BG diets showed that feed conversion ratio and malonaldehyde levels were significantly decreased (P < .05). Also, both L-137 and BG helped Nile tilapia to have high phagocytosis activity and relative expression of tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta (IL-1β) and interferon-gamma (INF-γ) genes. After A. hydrophila challenge, the intestinal villi epithelium of the L-137/BG group was intact and denser than the other groups. The hepatopancreas and spleen of the control group displayed severe necrosis in hepatocytes and congestion of blood sinusoids in addition to diffuse vacuolation. Regarding the L-137, BG and L-137/BG groups, there was a moderate and normal degree of vacuolation with focal necrosis and mild to moderate degree of congestion of blood sinusoids. Red blood cells, hemoglobin, and albumin showed meaningfully (P < .05) increased values in L-137 or L-137/BG groups. TNF-α, IL-1β, and INF-γ expressions were upregulated by L-137 and/or BG. The obtained results revealed the ability of L-137 and/or BG to protect Nile tilapia from the effects of A. hydrophila infection by the motivation of the immune, antioxidative, and antiinflammation responses. 2020-04-02T15:30:40Z 2020-04-02T15:30:40Z 2020-06-01 Journal 15322661 00345288 2-s2.0-85081938814 10.1016/j.rvsc.2020.03.019 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081938814&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/68592
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Veterinary
spellingShingle Veterinary
Mahmoud A.O. Dawood
Eman Moustafa Moustafa
Zizy I. Elbialy
Foad Farrag
Emad E.E. Lolo
Hanaa A. Abdel-Daim
Mohamed M. Abdel-Daim
Hien Van Doan
Lactobacillus plantarum L-137 and/or β-glucan impacted the histopathological, antioxidant, immune-related genes and resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila
description © 2020 Elsevier Ltd A trial was operated to assess the potential of using Lactobacillus plantarum L-137 (L-137) and/or β-glucan (BG) in improving the resistance of Nile tilapia against Aeromonas hydrophila. Control diet and 3 diets supplemented with L-137, BG or L-137 + BG were prepared. Final body weight, specific growth rate, superoxide dismutase, and catalase showed considerably (P < .05) increased values in L-137 or L-137/BG groups, while glutathione peroxidase increased significantly (P < .05) only in L-137/BG group. Fish fed L-137 and/or BG diets showed that feed conversion ratio and malonaldehyde levels were significantly decreased (P < .05). Also, both L-137 and BG helped Nile tilapia to have high phagocytosis activity and relative expression of tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta (IL-1β) and interferon-gamma (INF-γ) genes. After A. hydrophila challenge, the intestinal villi epithelium of the L-137/BG group was intact and denser than the other groups. The hepatopancreas and spleen of the control group displayed severe necrosis in hepatocytes and congestion of blood sinusoids in addition to diffuse vacuolation. Regarding the L-137, BG and L-137/BG groups, there was a moderate and normal degree of vacuolation with focal necrosis and mild to moderate degree of congestion of blood sinusoids. Red blood cells, hemoglobin, and albumin showed meaningfully (P < .05) increased values in L-137 or L-137/BG groups. TNF-α, IL-1β, and INF-γ expressions were upregulated by L-137 and/or BG. The obtained results revealed the ability of L-137 and/or BG to protect Nile tilapia from the effects of A. hydrophila infection by the motivation of the immune, antioxidative, and antiinflammation responses.
format Journal
author Mahmoud A.O. Dawood
Eman Moustafa Moustafa
Zizy I. Elbialy
Foad Farrag
Emad E.E. Lolo
Hanaa A. Abdel-Daim
Mohamed M. Abdel-Daim
Hien Van Doan
author_facet Mahmoud A.O. Dawood
Eman Moustafa Moustafa
Zizy I. Elbialy
Foad Farrag
Emad E.E. Lolo
Hanaa A. Abdel-Daim
Mohamed M. Abdel-Daim
Hien Van Doan
author_sort Mahmoud A.O. Dawood
title Lactobacillus plantarum L-137 and/or β-glucan impacted the histopathological, antioxidant, immune-related genes and resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila
title_short Lactobacillus plantarum L-137 and/or β-glucan impacted the histopathological, antioxidant, immune-related genes and resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila
title_full Lactobacillus plantarum L-137 and/or β-glucan impacted the histopathological, antioxidant, immune-related genes and resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila
title_fullStr Lactobacillus plantarum L-137 and/or β-glucan impacted the histopathological, antioxidant, immune-related genes and resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila
title_full_unstemmed Lactobacillus plantarum L-137 and/or β-glucan impacted the histopathological, antioxidant, immune-related genes and resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila
title_sort lactobacillus plantarum l-137 and/or β-glucan impacted the histopathological, antioxidant, immune-related genes and resistance of nile tilapia (oreochromis niloticus) against aeromonas hydrophila
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081938814&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68592
_version_ 1681426848059228160