Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn

Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn

Chiang Mai University (CMU) Journal of Natural Sciences is dedicated to the publication of original research in Sciences & Technology and the Health Sciences. Submissions are welcomed from CMU, as well as other Thai and foreign institutions. All submissions must be original research not previous...

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Main Authors: Kedsadaporn Junta, Orapin Saritnum, Ruangchai Juwattanasamran, Prawit Puddhanon, Darush Struss, Jumrearn Puttarach, Seksan Songchanthuek
Language:English
Published: Chiang Mai University 2020
Subjects:
Northern corn leaf blight
Sweet corn
SNP markers
Resistance
Online Access:http://cmuj.cmu.ac.th/uploads/journal_list_index/184108972.pdf
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68672
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-686722020-05-20T04:41:50Z Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn Kedsadaporn Junta Orapin Saritnum Ruangchai Juwattanasamran Prawit Puddhanon Darush Struss Jumrearn Puttarach Seksan Songchanthuek Northern corn leaf blight Sweet corn SNP markers Resistance Chiang Mai University (CMU) Journal of Natural Sciences is dedicated to the publication of original research in Sciences & Technology and the Health Sciences. Submissions are welcomed from CMU, as well as other Thai and foreign institutions. All submissions must be original research not previously published or simultaneously submitted for publication. Manuscripts are peer reviewed using the double -blinded review system by at least 2 reviewers before acceptance. The CMU Journal of Natural Sciences is published four times a year, in January, April, July and October. Northern corn leaf blight disease (NCLB) is a foliar disease of corn (Zea mays L.) caused by Exserohilum turcicum (Pass.). The Ht1, Ht2 and HtN1 genes in corn were found to control NCLB resistant traits. There has been an ongoing effort to identify various types of molecular markers associated with these genes in order to use them in marker-assisted selections. The objective of this study was to identify Single Nucleotide Polymorphism (SNP) markers which are associated with the Ht1 resistant gene. Ninety-three SNP markers were found in Ht1 regions, which are located on chromosome 2. Five SNP primers (MZSNP-0055106, MZSNP-0065744, MZSNP-0070164, MZSNP0063922, MZSNP-0073150) showed polymorphism between susceptible and resistant lines. The Chi-square test of genotypic data of 184 F2 plants (NT58WS6#4 x ChallengerS6-1) amplified by five markers fit a 1:2:1 ratio with Chi-square values of 0.82, 1.08, 1.08, 0.64 and 0.64 respectively. These 5 SNP primers may be useful as molecular markers for NCLB resistance in sweet corn. 2020-05-20T04:41:50Z 2020-05-20T04:41:50Z 2020 Chiang Mai University (CMU) Journal of Natural Sciences 19,1 (Jan-Mar 2020), p.155-162 2465-4337 http://cmuj.cmu.ac.th/uploads/journal_list_index/184108972.pdf http://cmuir.cmu.ac.th/jspui/handle/6653943832/68672 Eng Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
language English
topic Northern corn leaf blight
Sweet corn
SNP markers
Resistance
spellingShingle Northern corn leaf blight
Sweet corn
SNP markers
Resistance
Kedsadaporn Junta
Orapin Saritnum
Ruangchai Juwattanasamran
Prawit Puddhanon
Darush Struss
Jumrearn Puttarach
Seksan Songchanthuek
Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn
description Chiang Mai University (CMU) Journal of Natural Sciences is dedicated to the publication of original research in Sciences & Technology and the Health Sciences. Submissions are welcomed from CMU, as well as other Thai and foreign institutions. All submissions must be original research not previously published or simultaneously submitted for publication. Manuscripts are peer reviewed using the double -blinded review system by at least 2 reviewers before acceptance. The CMU Journal of Natural Sciences is published four times a year, in January, April, July and October.
author Kedsadaporn Junta
Orapin Saritnum
Ruangchai Juwattanasamran
Prawit Puddhanon
Darush Struss
Jumrearn Puttarach
Seksan Songchanthuek
author_facet Kedsadaporn Junta
Orapin Saritnum
Ruangchai Juwattanasamran
Prawit Puddhanon
Darush Struss
Jumrearn Puttarach
Seksan Songchanthuek
author_sort Kedsadaporn Junta
title Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn
title_short Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn
title_full Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn
title_fullStr Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn
title_full_unstemmed Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn
title_sort identification of single nucleotide polymorphism markers associated with northern corn leaf blight resistance in sweet corn
publisher Chiang Mai University
publishDate 2020
url http://cmuj.cmu.ac.th/uploads/journal_list_index/184108972.pdf
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68672
_version_ 1681752674499821568

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