Simple protocol to extract DNA from bone fragments-A preliminary study
Chiang Mai Veterinary Journal” (ISSN-print as 1685-9502 and ISSN-online as 2465-4604) before officially changing over to an electronic platform. The newly formatted journal is entitled “Veterinary Integrative Sciences” (ISSN as 2629-9968).
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| Language: | Tha |
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Faculty of Veterinary Medicine, Chiang Mai University
2020
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| Online Access: | https://he02.tci-thaijo.org/index.php/vis/article/view/146439/107964 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68717 |
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th-cmuir.6653943832-687172020-06-10T07:12:27Z Simple protocol to extract DNA from bone fragments-A preliminary study Siriwadee Chomdej Hataitip Tasena Piyamat Kongtung Korakot Nganvongpanit DNA extraction bone forensic sciences Chiang Mai Veterinary Journal” (ISSN-print as 1685-9502 and ISSN-online as 2465-4604) before officially changing over to an electronic platform. The newly formatted journal is entitled “Veterinary Integrative Sciences” (ISSN as 2629-9968). DNA extraction from bone samples is greatly useful for forensic applications but it is more diffi cult to be conducted compared with extracting DNA from other tissues due to rigid structure of the bones and contamination from the environment, such as, bacterial colonization within bone tissues. This research aimed to determine a simple and cost-effective protocol for extracting DNA from bone fragments. Canine leg bones were used as samples and canine Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene-specifi c primers were used to confi rm successful DNA extraction through PCR. We developed a protocol that was able to extract DNA from “fresh” as well as “old” bones that had been buried in soil or stored underwater in a pond for three months. Although the effectiveness decreased in the old bones, this protocol only requires common devices and chemicals readily available in general molecular laboratories and can be completed in less than 24 hours, offering an alternative lower-cost and less time intensive method for forensic research. 2020-06-10T07:12:27Z 2020-06-10T07:12:27Z 2015 Chiang Mai Veterinary Journal 13,1 (Jan-Apr. 2015) 51-57 0857-6920 https://he02.tci-thaijo.org/index.php/vis/article/view/146439/107964 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68717 Tha Faculty of Veterinary Medicine, Chiang Mai University |
| institution |
Chiang Mai University |
| building |
Chiang Mai University Library |
| continent |
Asia |
| country |
Thailand Thailand |
| content_provider |
Chiang Mai University Library |
| collection |
CMU Intellectual Repository |
| language |
Tha |
| topic |
DNA extraction bone forensic sciences |
| spellingShingle |
DNA extraction bone forensic sciences Siriwadee Chomdej Hataitip Tasena Piyamat Kongtung Korakot Nganvongpanit Simple protocol to extract DNA from bone fragments-A preliminary study |
| description |
Chiang Mai Veterinary Journal” (ISSN-print as 1685-9502 and ISSN-online as 2465-4604) before officially changing over to an electronic platform. The newly formatted journal is entitled “Veterinary Integrative Sciences” (ISSN as 2629-9968). |
| author |
Siriwadee Chomdej Hataitip Tasena Piyamat Kongtung Korakot Nganvongpanit |
| author_facet |
Siriwadee Chomdej Hataitip Tasena Piyamat Kongtung Korakot Nganvongpanit |
| author_sort |
Siriwadee Chomdej |
| title |
Simple protocol to extract DNA from bone fragments-A preliminary study |
| title_short |
Simple protocol to extract DNA from bone fragments-A preliminary study |
| title_full |
Simple protocol to extract DNA from bone fragments-A preliminary study |
| title_fullStr |
Simple protocol to extract DNA from bone fragments-A preliminary study |
| title_full_unstemmed |
Simple protocol to extract DNA from bone fragments-A preliminary study |
| title_sort |
simple protocol to extract dna from bone fragments-a preliminary study |
| publisher |
Faculty of Veterinary Medicine, Chiang Mai University |
| publishDate |
2020 |
| url |
https://he02.tci-thaijo.org/index.php/vis/article/view/146439/107964 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68717 |
| _version_ |
1681752682753163264 |