Search Information for Contributors Introducing the Journal Board of Journal of Science Contact address Select by Volumes SCImago Journal & Country Rank 0.39 2018 CiteScore 25th percentile Powered by Scopus Home > Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ
Chiang Mai Journal of Science
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Faculty of Science, Chiang Mai University
2020
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th-cmuir.6653943832-687332020-06-10T07:12:27Z Search Information for Contributors Introducing the Journal Board of Journal of Science Contact address Select by Volumes SCImago Journal & Country Rank 0.39 2018 CiteScore 25th percentile Powered by Scopus Home > Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ Phennapa Charoenwiwattanakij Jaturong Pratuangdejkul Sumet chongruchiroj Khanit Suwanborirux Chitti Thawai Jiraporn Chingunpitak Veena Satitpatipan actinomycin D antibacterial agent FtsZ marine Streptomyces Chiang Mai Journal of Science In the course of our investigation on antibacterial substances with FtsZ inhibitory effect, actinomycin D was isolated from the ethyl acetate extract of Streptomyces sp. LT3-17. The compound showed antibacterial activities. The MIC values of actinomycin D against Staphylococcus aureus ATCC 25923, Methicillin-resistant Staphylococcus aureus DMST 20654, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853 were 3.13, 0.39, 0.10, 300.00, and 500.00 μg/mL, respectively. The morphology study of E. coli JW0093 treated with the compound showed inhibitory effect on cell elongation. The inhibition of FtsZ activity by actinomycin D was shown as the inhibition of GTPase activity with IC50 value, 20.06 μM. The polymerization of E. coli FtsZ protein (EcFtsZ) treated with 0.01 μM actinomycin D showed the degree of polymerization ratio was less than 1.0 indicating the inhibitory potential of actinomycin D on FtsZ polymerization. In silico study was performed to predict binding mode of actinomycin D into nucleotide binding pocket of the homology model of EcFtsZ protein. From the results of these experiments, the isolation and elucidation methods of actinomycin D as well as its novel mechanism as FtsZ inhibitors have been discovered. 2020-06-10T07:12:27Z 2020-06-10T07:12:27Z 2020 Chiang Mai Journal of Science 47,3 (May 2020), p.362-377 2465-3845 https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10910 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68733 Eng Faculty of Science, Chiang Mai University |
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actinomycin D antibacterial agent FtsZ marine Streptomyces |
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actinomycin D antibacterial agent FtsZ marine Streptomyces Phennapa Charoenwiwattanakij Jaturong Pratuangdejkul Sumet chongruchiroj Khanit Suwanborirux Chitti Thawai Jiraporn Chingunpitak Veena Satitpatipan Search Information for Contributors Introducing the Journal Board of Journal of Science Contact address Select by Volumes SCImago Journal & Country Rank 0.39 2018 CiteScore 25th percentile Powered by Scopus Home > Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ |
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Chiang Mai Journal of Science |
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Phennapa Charoenwiwattanakij Jaturong Pratuangdejkul Sumet chongruchiroj Khanit Suwanborirux Chitti Thawai Jiraporn Chingunpitak Veena Satitpatipan |
| author_facet |
Phennapa Charoenwiwattanakij Jaturong Pratuangdejkul Sumet chongruchiroj Khanit Suwanborirux Chitti Thawai Jiraporn Chingunpitak Veena Satitpatipan |
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Phennapa Charoenwiwattanakij |
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Effect of Actinomycin D Isolated From the Cultured Broth of Marine Streptomyces spp. on Cell Division Protein FtsZ |
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home > effect of actinomycin d isolated from the cultured broth of marine streptomyces spp. on cell division protein ftsz
effect of actinomycin d isolated from the cultured broth of marine streptomyces spp. on cell division protein ftsz |
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Faculty of Science, Chiang Mai University |
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2020 |
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https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10910 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68733 |
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1681752685694418944 |