Cloning and expression of recombinant O-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris

Chiang Mai Veterinary Journal” (ISSN-print as 1685-9502 and ISSN-online as 2465-4604) before officially changing over to an electronic platform. The newly formatted journal is entitled “Veterinary Integrative Sciences” (ISSN as 2629-9968).

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Main Authors:	Kanruethai Wongsawan, Ronachai Pratanaphon, Sophit Khanthawong, Prasit Thraravichitkul
Language:	English
Published:	Faculty of Veterinary Medicine 2020
Subjects:	human Streptococcus suis recombinant O-acetylserine lyase recombinant protein
Online Access:	https://he02.tci-thaijo.org/index.php/vis/article/view/146564/108054 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68739
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Institution:	Chiang Mai University
Language:	English

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spelling	th-cmuir.6653943832-687392020-06-10T07:12:27Z Cloning and expression of recombinant O-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris Kanruethai Wongsawan Ronachai Pratanaphon Sophit Khanthawong Prasit Thraravichitkul human Streptococcus suis recombinant O-acetylserine lyase recombinant protein Chiang Mai Veterinary Journal” (ISSN-print as 1685-9502 and ISSN-online as 2465-4604) before officially changing over to an electronic platform. The newly formatted journal is entitled “Veterinary Integrative Sciences” (ISSN as 2629-9968). Streptococcus suis is recognized worldwide as an important swine pathogen, which occasionally infects humans and causes fatal illnesses. O-acetylserine lyase (OASL) is responsible for the ﬁ nal step of the cysteine biosynthesis, a key step in bacterial sulfur metabolism and offers a means by which inorganic sulfur maybe incorporated into cellular components. Recently, OASL was reported as a putative virulence-associated factor and immunogenic protein in S. suis. And this suggests that it may be involved in pathogenesis. In this study, the gene coding for OASL was cloned from a clinical S. suis serotype 2 strain. A full-length gene encoding OASL was ampliﬁ ed by the OASL gene-speciﬁ c primer. The ampliﬁ ed fragments were cloned into pGAPZA vector and expression as a His-tagged recombinant protein in Pichia pastoris. The expressed sample collected from the supernatant was ﬁ rst analyzed by SDS-PAGE. After that the recombinant proteins were detected by Ni2+-NTA enzyme conjugate. The Western immunoblotting showed that either antibodies present in patient serum suffered from S. suis infection or rabbit serum raised against whole cell lysate of homologous and heterologous strains can bind to recombinant OASL. These results verify that OASL had antigenicity and guide for evaluating its antigenic potential in further studies. 2020-06-10T07:12:27Z 2020-06-10T07:12:27Z 2014 Chiang Mai Veterinary Journal 12,2 (May - Aug. 2014) 121-129 0857-6921 https://he02.tci-thaijo.org/index.php/vis/article/view/146564/108054 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68739 Eng Faculty of Veterinary Medicine
institution	Chiang Mai University
building	Chiang Mai University Library
continent	Asia
country	Thailand Thailand
content_provider	Chiang Mai University Library
collection	CMU Intellectual Repository
language	English
topic	human Streptococcus suis recombinant O-acetylserine lyase recombinant protein
spellingShingle	human Streptococcus suis recombinant O-acetylserine lyase recombinant protein Kanruethai Wongsawan Ronachai Pratanaphon Sophit Khanthawong Prasit Thraravichitkul Cloning and expression of recombinant O-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris
description	Chiang Mai Veterinary Journal” (ISSN-print as 1685-9502 and ISSN-online as 2465-4604) before officially changing over to an electronic platform. The newly formatted journal is entitled “Veterinary Integrative Sciences” (ISSN as 2629-9968).
author	Kanruethai Wongsawan Ronachai Pratanaphon Sophit Khanthawong Prasit Thraravichitkul
author_facet	Kanruethai Wongsawan Ronachai Pratanaphon Sophit Khanthawong Prasit Thraravichitkul
author_sort	Kanruethai Wongsawan
title	Cloning and expression of recombinant O-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris
title_short	Cloning and expression of recombinant O-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris
title_full	Cloning and expression of recombinant O-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris
title_fullStr	Cloning and expression of recombinant O-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris
title_full_unstemmed	Cloning and expression of recombinant O-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris
title_sort	cloning and expression of recombinant o-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris
publisher	Faculty of Veterinary Medicine
publishDate	2020
url	https://he02.tci-thaijo.org/index.php/vis/article/view/146564/108054 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68739
_version_	1681752686769209344

Cloning and expression of recombinant O-acetylserine lyase from human streptococcus suis serotype 2 in pichia pastoris

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