Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3
Chiang Mai Journal of Science
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Faculty of Science, Chiang Mai University
2020
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| Online Access: | https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10912 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68741 |
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th-cmuir.6653943832-687412020-06-10T07:12:27Z Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3 Wasan Seemakram Santhaya Boonrung Urachart Kokaew Tadanori Aimi Sophon Boonlue elephant dung thermo-alkaliphilic fungi response surface methodology Chiang Mai Journal of Science The thermoalkaliphilic cellulase-free xylanase-producing fungi, Thermomyces dupontii KKU−CLD−E2−3 was isolated from elephant dung. The production of thermo-alkali-stable and cellulase-free xylanase of the fungus was optimized in solid-state fermentation using statistical approaches. Response surface methodology (RSM) using central composite design (CCD) was employed to optimize conditions. The maximum xylanase production was observed at a 1.72% nitrogen concentration, initial pH of 10.54, initial moisture content of 74.73% and under incubation for 8 days at a temperature of 43.93 ºC. The maximum xylanase activity was evaluated at pH 11.0 and retained over 80% of the original activity within a pH range of 7.0–10.0 after incubation at 4 ºC for 24 h. In addition, the optimum temperature for xylanase activity was 80 ºC. The enzyme also retained more than 80% of its residual activity after heated at 60 ºC for 90 min. Therefore, its properties demonstrate significant promise for use in pulp bleaching for the paper industry; for which this study will be the focus of future investigation. 2020-06-10T07:12:27Z 2020-06-10T07:12:27Z 2020 Chiang Mai Journal of Science 47,3 (May 2020), p.391-402 2465-3845 https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10912 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68741 Eng Faculty of Science, Chiang Mai University |
| institution |
Chiang Mai University |
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Chiang Mai University Library |
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Asia |
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Thailand Thailand |
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Chiang Mai University Library |
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CMU Intellectual Repository |
| language |
English |
| topic |
elephant dung thermo-alkaliphilic fungi response surface methodology |
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elephant dung thermo-alkaliphilic fungi response surface methodology Wasan Seemakram Santhaya Boonrung Urachart Kokaew Tadanori Aimi Sophon Boonlue Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3 |
| description |
Chiang Mai Journal of Science |
| author |
Wasan Seemakram Santhaya Boonrung Urachart Kokaew Tadanori Aimi Sophon Boonlue |
| author_facet |
Wasan Seemakram Santhaya Boonrung Urachart Kokaew Tadanori Aimi Sophon Boonlue |
| author_sort |
Wasan Seemakram |
| title |
Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3 |
| title_short |
Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3 |
| title_full |
Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3 |
| title_fullStr |
Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3 |
| title_full_unstemmed |
Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3 |
| title_sort |
optimization of culture conditions for xylanase production from cellulase-free xylanase-producing thermophilic fungus, thermomyces dupontii kku−cld−e2−3 |
| publisher |
Faculty of Science, Chiang Mai University |
| publishDate |
2020 |
| url |
https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10912 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68741 |
| _version_ |
1681752687108947968 |