Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta
Chiang Mai Journal of Science
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Faculty of Science, Chiang Mai University
2020
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| Online Access: | https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10920 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68748 |
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th-cmuir.6653943832-687482020-06-10T07:12:28Z Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta Gui-Sheng Zhou Yu-Ping Tang Yao Li Jin-Ao Duan Ginkgo biloba sarcotesta polysaccharides ultrasonic assisted enzymatic extraction response surface methodology antioxidant activity Chiang Mai Journal of Science The combination of Plackett–Burman design (PBD) and rotatable central composite design (RCCD) was used to optimize ultrasonic assisted enzymatic extraction (UAEE) for achieving maximal recovery of antioxidant polysaccharides from waste Ginkgo biloba sarcotesta (GBSP). After the combination of PBD and RCCD, the resulting optimal UAEE conditions were as follows: UAE power of 239 W, time of 31 min, cellulase concentration of 3.2%, UAE temperature of 60°C, pH of 4.5, solvent-to-solid of 30 mL/g and sample particle size of 60 mesh. Under the optimum conditions, the experimental yield of GBSP was 7.71 ± 0.53 % (n = 3) and ferric-reducing antioxidant capacity values of GBSP was 22.43 ± 1.93 mmol Fe2+/g (n = 3), which were close to the above predicted values. Moreover, the antioxidant activities of GBSPUAEE obtained by UAEE compared with GBSPHWE obtained by hot water extraction (HWE), the antioxidant activities obtained using GBSPUAEE were significantly higher than those GBSPHWE. Additionally, the carbohydrate content, monosaccharide composition and IR spectrum of the GBSPUAEE were also investigated. Therefore, the present study provided an advisable method of UAEE for extraction of antioxidant GBSP from waste Ginkgo biloba resource. Keywords: Ginkgo biloba sarcotesta, polysaccharides, ultrasonic assisted enzymatic extraction, response surface methodology, antioxidant activity 2020-06-10T07:12:28Z 2020-06-10T07:12:28Z 2020 Chiang Mai Journal of Science 47,3 (May 2020), p.499-512 2465-3845 https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10920 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68748 Eng Faculty of Science, Chiang Mai University |
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Ginkgo biloba sarcotesta polysaccharides ultrasonic assisted enzymatic extraction response surface methodology antioxidant activity |
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Ginkgo biloba sarcotesta polysaccharides ultrasonic assisted enzymatic extraction response surface methodology antioxidant activity Gui-Sheng Zhou Yu-Ping Tang Yao Li Jin-Ao Duan Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta |
| description |
Chiang Mai Journal of Science |
| author |
Gui-Sheng Zhou Yu-Ping Tang Yao Li Jin-Ao Duan |
| author_facet |
Gui-Sheng Zhou Yu-Ping Tang Yao Li Jin-Ao Duan |
| author_sort |
Gui-Sheng Zhou |
| title |
Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta |
| title_short |
Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta |
| title_full |
Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta |
| title_fullStr |
Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta |
| title_full_unstemmed |
Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta |
| title_sort |
multi-response optimization of ultrasonic assisted enzymatic extraction of antioxidant polysaccharides from waste ginkgo biloba sarcotesta |
| publisher |
Faculty of Science, Chiang Mai University |
| publishDate |
2020 |
| url |
https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=10920 http://cmuir.cmu.ac.th/jspui/handle/6653943832/68748 |
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