Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis
Aim To measure the levels of hCAP18/LL-37 in gingival crevicular fluid from patients with periodontal diseases compared with healthy controls and to determine the correlation between hCAP18/LL-37 and chondroitin sulphate (CS) levels in patients with periodontitis. Material and Methods Gingival crevi...
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th-cmuir.6653943832-6882014-08-29T09:01:56Z Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis Makeudom A. Kulpawaropas S. Montreekachon P. Khongkhunthian S. Sastraruji T. Pothacharoen P. Kongtawelert P. Krisanaprakornkit S. Aim To measure the levels of hCAP18/LL-37 in gingival crevicular fluid from patients with periodontal diseases compared with healthy controls and to determine the correlation between hCAP18/LL-37 and chondroitin sulphate (CS) levels in patients with periodontitis. Material and Methods Gingival crevicular fluid samples from 51 patients and 25 healthy volunteers were analysed for the hCAP18/LL-37 levels by immunoblotting and were determined for the CS levels by the competitive enzyme-linked immunosorbent assay. Results Tris buffer pH 9.85 was selected to recover hCAP18/LL-37 from Periopaper strips, in which the percentages of recovery were around 70%. The median levels of hCAP18/LL-37 in the aggressive and the chronic periodontitis (CP) groups were significantly greater than those in the gingivitis and the healthy groups (p < 0.05). Significant correlations between the unprocessed 18-kDa fragment and CS levels (r = 0.650; p < 0.001) and between the mature 4.6-kDa fragment and CS levels (r = 0.502; p < 0.001) were observed only in the CP group. Conclusion The significant correlations between the hCAP18/LL-37 and the CS levels were found in CP, but not in aggressive periodontitis. The presence versus absence of such correlations may be clinically applicable to help clinicians distinguish between two distinct types of periodontitis. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. 2014-08-29T09:01:56Z 2014-08-29T09:01:56Z 2014 Article 03036979 10.1111/jcpe.12216 JCPED http://www.scopus.com/inward/record.url?eid=2-s2.0-84893507456&partnerID=40&md5=086bf64ef9b2a40cad99782d9723e504 http://cmuir.cmu.ac.th/handle/6653943832/688 English |
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Aim To measure the levels of hCAP18/LL-37 in gingival crevicular fluid from patients with periodontal diseases compared with healthy controls and to determine the correlation between hCAP18/LL-37 and chondroitin sulphate (CS) levels in patients with periodontitis. Material and Methods Gingival crevicular fluid samples from 51 patients and 25 healthy volunteers were analysed for the hCAP18/LL-37 levels by immunoblotting and were determined for the CS levels by the competitive enzyme-linked immunosorbent assay. Results Tris buffer pH 9.85 was selected to recover hCAP18/LL-37 from Periopaper strips, in which the percentages of recovery were around 70%. The median levels of hCAP18/LL-37 in the aggressive and the chronic periodontitis (CP) groups were significantly greater than those in the gingivitis and the healthy groups (p < 0.05). Significant correlations between the unprocessed 18-kDa fragment and CS levels (r = 0.650; p < 0.001) and between the mature 4.6-kDa fragment and CS levels (r = 0.502; p < 0.001) were observed only in the CP group. Conclusion The significant correlations between the hCAP18/LL-37 and the CS levels were found in CP, but not in aggressive periodontitis. The presence versus absence of such correlations may be clinically applicable to help clinicians distinguish between two distinct types of periodontitis. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
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Makeudom A. Kulpawaropas S. Montreekachon P. Khongkhunthian S. Sastraruji T. Pothacharoen P. Kongtawelert P. Krisanaprakornkit S. |
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Makeudom A. Kulpawaropas S. Montreekachon P. Khongkhunthian S. Sastraruji T. Pothacharoen P. Kongtawelert P. Krisanaprakornkit S. Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis |
author_facet |
Makeudom A. Kulpawaropas S. Montreekachon P. Khongkhunthian S. Sastraruji T. Pothacharoen P. Kongtawelert P. Krisanaprakornkit S. |
author_sort |
Makeudom A. |
title |
Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis |
title_short |
Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis |
title_full |
Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis |
title_fullStr |
Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis |
title_full_unstemmed |
Positive correlations between hCAP18/LL-37 and chondroitin sulphate levels in chronic periodontitis |
title_sort |
positive correlations between hcap18/ll-37 and chondroitin sulphate levels in chronic periodontitis |
publishDate |
2014 |
url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-84893507456&partnerID=40&md5=086bf64ef9b2a40cad99782d9723e504 http://cmuir.cmu.ac.th/handle/6653943832/688 |
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1681419530110238720 |