Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)

Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)

Aerobic bacteria were isolated from the midgut of Apis cerana indica on five different media. The highest count (5.4 × 107 colony forming units (CFU/g)) was obtained on Eosin methylene blue (EMB) medium. Fifty-eight isolates were purified and identified using biochemical tests, and 16S rRNA gene seq...

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Main Authors: Disayathanoowat T., Yoshiyama M., Kimura K., Chantawannakul P.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84867750488&partnerID=40&md5=dd9be2de6df0f28baa399eea853ed6c2
http://cmuir.cmu.ac.th/handle/6653943832/6929
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-69292014-08-30T03:51:24Z Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica) Disayathanoowat T. Yoshiyama M. Kimura K. Chantawannakul P. Aerobic bacteria were isolated from the midgut of Apis cerana indica on five different media. The highest count (5.4 × 107 colony forming units (CFU/g)) was obtained on Eosin methylene blue (EMB) medium. Fifty-eight isolates were purified and identified using biochemical tests, and 16S rRNA gene sequences were analysed. Two groups of Gamma-proteobacteria and Firmicutes were found. The most abundant species were Klebsiella pneumoniae, Enterobacter cloacae and K. oxytoca. To determine their role in nutrition, enzymes produced from the bacterial isolates were assayed. Protease and lipase activities were detected in some bacterial isolates. The assimilation of fourteen carbon substrates was studied. D-mannose and raffinose showed the highest utilization. Paenibacillus larvae, the cause of American foulbrood disease, was inhibited by some members of Klebsiella. 2014-08-30T03:51:24Z 2014-08-30T03:51:24Z 2012 Article 218839 10.3896/IBRA.1.51.4.04 JACRA http://www.scopus.com/inward/record.url?eid=2-s2.0-84867750488&partnerID=40&md5=dd9be2de6df0f28baa399eea853ed6c2 http://cmuir.cmu.ac.th/handle/6653943832/6929 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Aerobic bacteria were isolated from the midgut of Apis cerana indica on five different media. The highest count (5.4 × 107 colony forming units (CFU/g)) was obtained on Eosin methylene blue (EMB) medium. Fifty-eight isolates were purified and identified using biochemical tests, and 16S rRNA gene sequences were analysed. Two groups of Gamma-proteobacteria and Firmicutes were found. The most abundant species were Klebsiella pneumoniae, Enterobacter cloacae and K. oxytoca. To determine their role in nutrition, enzymes produced from the bacterial isolates were assayed. Protease and lipase activities were detected in some bacterial isolates. The assimilation of fourteen carbon substrates was studied. D-mannose and raffinose showed the highest utilization. Paenibacillus larvae, the cause of American foulbrood disease, was inhibited by some members of Klebsiella.
format Article
author Disayathanoowat T.
Yoshiyama M.
Kimura K.
Chantawannakul P.
spellingShingle Disayathanoowat T.
Yoshiyama M.
Kimura K.
Chantawannakul P.
Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
author_facet Disayathanoowat T.
Yoshiyama M.
Kimura K.
Chantawannakul P.
author_sort Disayathanoowat T.
title Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_short Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_full Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_fullStr Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_full_unstemmed Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)
title_sort isolation and characterization of bacteria from the midgut of the asian honey bee (apis cerana indica)
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84867750488&partnerID=40&md5=dd9be2de6df0f28baa399eea853ed6c2
http://cmuir.cmu.ac.th/handle/6653943832/6929
_version_ 1681420705797767168

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