Hydrogen Sulfide Removal from Biogas Using Immobilized Sulfur Oxidizing Bacterium Paracoccus versutus CM1 in Biofilters
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science...
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Faculty of Science, Chiang Mai University
2020
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th-cmuir.6653943832-698972020-10-08T08:36:16Z Hydrogen Sulfide Removal from Biogas Using Immobilized Sulfur Oxidizing Bacterium Paracoccus versutus CM1 in Biofilters Dolruedee Jirachaisakdeacha Ladapa Kumdhitiahutsawakul Patiroop Pholchan Uthen Kantha Wasu Pathom-aree Sakunnee Bovonsombut sulfur oxidizing bacteria Paracoccus sp. hydrogen sulfide removal biogas immobilized bacterial cells biofilter The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so. Hydrogen sulfide (H2S), a gas commonly occurring in biogas systems, is harmful to health and causes problems in biogas utilization. Removal of H2S will improve the quality of biogas. This study was aimed to establish an efficient biological process for H2S removal using immobilized sulfur oxidizing bacteria (SOB) in a biofilter. Bacteria were isolated from H2S treatment systems, using modified Thiosulfate medium. Among the eighteen isolates obtained, one isolate, CM1, was selected for further optimization of the H2S removal process based on its rapid growth and metabolic rate observed within 72 hours of culturing. The optimum conditions to achieve the highest cell numbers of CM1 were determined to be at the temperature of 37C, pH 7 and under strictly aerobic or microaerobic condition. Six supporting materials (porous glass, granular activated carbon, charcoal, polyurethane foam, alginate and alginate mixed with bacterial cellulose) were used to immobilize cells of CM1 for removal of H2S. The immobilized cell numbers were found in the range of approximately 8-11 log CFU/g, with the highest number (10.70 log CFU/g) obtained when polyurethane foam was used as the supporting material. Complete (100%) removal of H2S in the laboratory-scale system was achieved when using porous glass, granular activated carbon, charcoal, polyurethane foam, and alginate as biofilters; while the alginate mixed with bacterial cellulose had a maximum removal efficiency of 77%. CM1 was identified as Paracoccus versutus, using 16S rRNA gene sequencing, and analysis of soxB gene sequence also revealed an identical translated amino acid sequence to the thiosulfohydrolase SoxB sequence of a Paracoccus species. 2020-10-08T08:36:16Z 2020-10-08T08:36:16Z 2020 Chiang Mai Journal of Science 47,5 (Sep 2020), p.872-886 2465-3845 https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=11188 http://cmuir.cmu.ac.th/jspui/handle/6653943832/69897 Eng Faculty of Science, Chiang Mai University |
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sulfur oxidizing bacteria Paracoccus sp. hydrogen sulfide removal biogas immobilized bacterial cells biofilter |
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sulfur oxidizing bacteria Paracoccus sp. hydrogen sulfide removal biogas immobilized bacterial cells biofilter Dolruedee Jirachaisakdeacha Ladapa Kumdhitiahutsawakul Patiroop Pholchan Uthen Kantha Wasu Pathom-aree Sakunnee Bovonsombut Hydrogen Sulfide Removal from Biogas Using Immobilized Sulfur Oxidizing Bacterium Paracoccus versutus CM1 in Biofilters |
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The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so. |
author |
Dolruedee Jirachaisakdeacha Ladapa Kumdhitiahutsawakul Patiroop Pholchan Uthen Kantha Wasu Pathom-aree Sakunnee Bovonsombut |
author_facet |
Dolruedee Jirachaisakdeacha Ladapa Kumdhitiahutsawakul Patiroop Pholchan Uthen Kantha Wasu Pathom-aree Sakunnee Bovonsombut |
author_sort |
Dolruedee Jirachaisakdeacha |
title |
Hydrogen Sulfide Removal from Biogas Using Immobilized Sulfur Oxidizing Bacterium Paracoccus versutus CM1 in Biofilters |
title_short |
Hydrogen Sulfide Removal from Biogas Using Immobilized Sulfur Oxidizing Bacterium Paracoccus versutus CM1 in Biofilters |
title_full |
Hydrogen Sulfide Removal from Biogas Using Immobilized Sulfur Oxidizing Bacterium Paracoccus versutus CM1 in Biofilters |
title_fullStr |
Hydrogen Sulfide Removal from Biogas Using Immobilized Sulfur Oxidizing Bacterium Paracoccus versutus CM1 in Biofilters |
title_full_unstemmed |
Hydrogen Sulfide Removal from Biogas Using Immobilized Sulfur Oxidizing Bacterium Paracoccus versutus CM1 in Biofilters |
title_sort |
hydrogen sulfide removal from biogas using immobilized sulfur oxidizing bacterium paracoccus versutus cm1 in biofilters |
publisher |
Faculty of Science, Chiang Mai University |
publishDate |
2020 |
url |
https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=11188 http://cmuir.cmu.ac.th/jspui/handle/6653943832/69897 |
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1681752803788193792 |