Antioxidant and Anti-inflammatory Activities of Ethanolic Extract from Hoya kerrii Craib

The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science...

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Main Authors: Patcharawan Sittisart, Benjawan Dunkhunthod, Chokchai Chuea-nongthon
Language:English
Published: Faculty of Science, Chiang Mai University 2020
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Online Access:https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=11191
http://cmuir.cmu.ac.th/jspui/handle/6653943832/69899
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-698992020-10-08T08:36:16Z Antioxidant and Anti-inflammatory Activities of Ethanolic Extract from Hoya kerrii Craib Patcharawan Sittisart Benjawan Dunkhunthod Chokchai Chuea-nongthon Hoya kerrii phytochemical antioxidant inflammatory mediator RAW264.7 The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so. Hoya kerrii Craib (HK) has long been used as folk medicines for curing inflammatory-related diseases. This study aimed to investigate the phytochemical constituents, antioxidant and anti-inflammatory effects of 95% ethanol extract of HK (HKE). The high-performance liquid chromatography diode array detection mass spectrometry (HPLC-DAD-MS) analyzes of polyphenolic composition indicated the presence of gallic acid, catechin, tannic acid, isoquercetin, kaempferol, quercetin, rutin, genistein, daidzein, genistin and daidzin in HKE as bioactive compounds. The extract exhibited antioxidant properties based on 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power (FRAP) assays. HKE also alleviated the tert-butyl hydroperoxide (tBuOOH)-induced reactive oxygen species (ROS) generation in RAW264.7 cells as measured by using 2’,7’-dichlorofluorescin-diacetate (DCFH-DA) assay. HKE suppressed LPS plus IFN-γ-induced nitric oxide (NO) production in RAW264.7 cells as determined by using Griess reagent. HKE exhibited the immunomodulatory effects by suppressing the secretion of pro-inflammatory cytokines TNF-α and IL-6 from RAW264.7 cells activated by LPS plus IFN-γ which was performed by enzyme-linked immunosorbent assay (ELISA). Collectively, these results suggest that HK may be applied as a promising antioxidant and anti-inflammatory natural agent through modulation of inflammatory mediators NO and cytokines such as IL-6 and TNF-α. 2020-10-08T08:36:16Z 2020-10-08T08:36:16Z 2020 Chiang Mai Journal of Science 47,5 (Sep 2020), p.912-925 2465-3845 https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=11191 http://cmuir.cmu.ac.th/jspui/handle/6653943832/69899 Eng Faculty of Science, Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
language English
topic Hoya kerrii
phytochemical
antioxidant
inflammatory mediator
RAW264.7
spellingShingle Hoya kerrii
phytochemical
antioxidant
inflammatory mediator
RAW264.7
Patcharawan Sittisart
Benjawan Dunkhunthod
Chokchai Chuea-nongthon
Antioxidant and Anti-inflammatory Activities of Ethanolic Extract from Hoya kerrii Craib
description The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.
author Patcharawan Sittisart
Benjawan Dunkhunthod
Chokchai Chuea-nongthon
author_facet Patcharawan Sittisart
Benjawan Dunkhunthod
Chokchai Chuea-nongthon
author_sort Patcharawan Sittisart
title Antioxidant and Anti-inflammatory Activities of Ethanolic Extract from Hoya kerrii Craib
title_short Antioxidant and Anti-inflammatory Activities of Ethanolic Extract from Hoya kerrii Craib
title_full Antioxidant and Anti-inflammatory Activities of Ethanolic Extract from Hoya kerrii Craib
title_fullStr Antioxidant and Anti-inflammatory Activities of Ethanolic Extract from Hoya kerrii Craib
title_full_unstemmed Antioxidant and Anti-inflammatory Activities of Ethanolic Extract from Hoya kerrii Craib
title_sort antioxidant and anti-inflammatory activities of ethanolic extract from hoya kerrii craib
publisher Faculty of Science, Chiang Mai University
publishDate 2020
url https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=11191
http://cmuir.cmu.ac.th/jspui/handle/6653943832/69899
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