Novel endophytic fungi with fungicidal metabolites suppress sclerotium disease

© 2020 Elsevier B.V. This study is aimed to investigate the antifungal activity of endophytic fungi isolated from Jerusalem artichoke (Helianthus tuberosus) and Ginger (Zingiber officinale) and Stemona root (Stemona tuberosa) against causative agent of southern stem rot Sclerotium rolfsii. The dual...

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Main Authors: Thanapat Suebrasri, Apisara Somteds, Hiroyuki Harada, Somdej Kanokmedhakul, Sanun Jogloy, Jindarat Ekprasert, Saisamorn Lumyong, Sophon Boonlue
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/69932
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spelling th-cmuir.6653943832-699322020-10-14T08:22:16Z Novel endophytic fungi with fungicidal metabolites suppress sclerotium disease Thanapat Suebrasri Apisara Somteds Hiroyuki Harada Somdej Kanokmedhakul Sanun Jogloy Jindarat Ekprasert Saisamorn Lumyong Sophon Boonlue Agricultural and Biological Sciences © 2020 Elsevier B.V. This study is aimed to investigate the antifungal activity of endophytic fungi isolated from Jerusalem artichoke (Helianthus tuberosus) and Ginger (Zingiber officinale) and Stemona root (Stemona tuberosa) against causative agent of southern stem rot Sclerotium rolfsii. The dual assay results showed that out of 110 fungal endophytes, Diaporthe phaseolorum BUP3/1 and Macrophomina phaseolina BUP2/3, isolated from Jerusalem artichoke and Daldinia eschscholtzii 2NTYL11 and Trichoderma erinaceum ST-KKU2, isolated from Stemona root and ginger could effectively inhibited the mycelial growth of S. rolfsii at 76.00, 41.20, 66.67 and 63.63%, respectively. The β-1,3 glucanase and chitinase activities of all endophytic fungi was determined which reached the maximum after 2 and 7 days of incubation, respectively. In addition, Scanning Electron Microscope (SEM) observation showed that T. erinaceum ST-KKU2 has predatory mechanism aginst S. rolfsii via coiling and invasion. Interestingly, our endophytic fungi T. erinaceum ST-KKU2 and D. eschscholtzii 2NTYL11 were the first species in their genera to produce polyketides group as 6-n-pentyl-2H-pyran-2-one (6PAP) and 2,3-dihydro-5-hydroxy-2-methyl-4H-1-benzopyran-4-one (DHMB), respectively, to control S. rolfsii. Moreover, the antagonistic mechanisms of all endophytes were determined in vivo under greenhouse condition. The endophytic fungi, T. erinaceum ST-KKU2 and D. eschscholtzii 2NTYL11 also showed high level of reduction of southern stem rot disease by 58.14% under pot experiment. Such effects were also higher than the effects of other endophytic fungi isolated from Jerusalem artichoke when tested under greenhouse condition. Our experiments showed the fungal endophytes isolated have potential for further development as a biocontrol agent in the future. 2020-10-14T08:22:16Z 2020-10-14T08:22:16Z 2020-12-01 Journal 24522198 2-s2.0-85091527715 10.1016/j.rhisph.2020.100250 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85091527715&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/69932
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Thanapat Suebrasri
Apisara Somteds
Hiroyuki Harada
Somdej Kanokmedhakul
Sanun Jogloy
Jindarat Ekprasert
Saisamorn Lumyong
Sophon Boonlue
Novel endophytic fungi with fungicidal metabolites suppress sclerotium disease
description © 2020 Elsevier B.V. This study is aimed to investigate the antifungal activity of endophytic fungi isolated from Jerusalem artichoke (Helianthus tuberosus) and Ginger (Zingiber officinale) and Stemona root (Stemona tuberosa) against causative agent of southern stem rot Sclerotium rolfsii. The dual assay results showed that out of 110 fungal endophytes, Diaporthe phaseolorum BUP3/1 and Macrophomina phaseolina BUP2/3, isolated from Jerusalem artichoke and Daldinia eschscholtzii 2NTYL11 and Trichoderma erinaceum ST-KKU2, isolated from Stemona root and ginger could effectively inhibited the mycelial growth of S. rolfsii at 76.00, 41.20, 66.67 and 63.63%, respectively. The β-1,3 glucanase and chitinase activities of all endophytic fungi was determined which reached the maximum after 2 and 7 days of incubation, respectively. In addition, Scanning Electron Microscope (SEM) observation showed that T. erinaceum ST-KKU2 has predatory mechanism aginst S. rolfsii via coiling and invasion. Interestingly, our endophytic fungi T. erinaceum ST-KKU2 and D. eschscholtzii 2NTYL11 were the first species in their genera to produce polyketides group as 6-n-pentyl-2H-pyran-2-one (6PAP) and 2,3-dihydro-5-hydroxy-2-methyl-4H-1-benzopyran-4-one (DHMB), respectively, to control S. rolfsii. Moreover, the antagonistic mechanisms of all endophytes were determined in vivo under greenhouse condition. The endophytic fungi, T. erinaceum ST-KKU2 and D. eschscholtzii 2NTYL11 also showed high level of reduction of southern stem rot disease by 58.14% under pot experiment. Such effects were also higher than the effects of other endophytic fungi isolated from Jerusalem artichoke when tested under greenhouse condition. Our experiments showed the fungal endophytes isolated have potential for further development as a biocontrol agent in the future.
format Journal
author Thanapat Suebrasri
Apisara Somteds
Hiroyuki Harada
Somdej Kanokmedhakul
Sanun Jogloy
Jindarat Ekprasert
Saisamorn Lumyong
Sophon Boonlue
author_facet Thanapat Suebrasri
Apisara Somteds
Hiroyuki Harada
Somdej Kanokmedhakul
Sanun Jogloy
Jindarat Ekprasert
Saisamorn Lumyong
Sophon Boonlue
author_sort Thanapat Suebrasri
title Novel endophytic fungi with fungicidal metabolites suppress sclerotium disease
title_short Novel endophytic fungi with fungicidal metabolites suppress sclerotium disease
title_full Novel endophytic fungi with fungicidal metabolites suppress sclerotium disease
title_fullStr Novel endophytic fungi with fungicidal metabolites suppress sclerotium disease
title_full_unstemmed Novel endophytic fungi with fungicidal metabolites suppress sclerotium disease
title_sort novel endophytic fungi with fungicidal metabolites suppress sclerotium disease
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85091527715&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/69932
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