Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The present investigation aimed to determine the fungal toxicity of Isaria tenuipes (My-It) against the dengue mosquito vector Aedes aegypti L. and its non-target impact against the aquatic predator Toxorhynchites splendens. Lethal concentrat...

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Main Authors: Sengodan Karthi, Prabhakaran Vasantha-Srinivasan, Raja Ganesan, Venkatachalam Ramasamy, Sengottayan Senthil-Nathan, Hanem F. Khater, Narayanaswamy Radhakrishnan, Kesavan Amala, Tae Jin Kim, Mohamed A. El-Sheikh, Patcharin Krutmuang
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Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/69954
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spelling th-cmuir.6653943832-699542020-10-14T08:40:54Z Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators Sengodan Karthi Prabhakaran Vasantha-Srinivasan Raja Ganesan Venkatachalam Ramasamy Sengottayan Senthil-Nathan Hanem F. Khater Narayanaswamy Radhakrishnan Kesavan Amala Tae Jin Kim Mohamed A. El-Sheikh Patcharin Krutmuang Agricultural and Biological Sciences Medicine © 2020 by the authors. Licensee MDPI, Basel, Switzerland. The present investigation aimed to determine the fungal toxicity of Isaria tenuipes (My-It) against the dengue mosquito vector Aedes aegypti L. and its non-target impact against the aquatic predator Toxorhynchites splendens. Lethal concentrations (LC50 and LC90) of My-It were observed in 2.27 and 2.93 log ppm dosages, respectively. The sub-lethal dosage (My-It-1 × 104 conidia/mL) displayed a significant oviposition deterrence index and also blocked the fecundity rate of dengue mosquitos in a dose-dependent manner. The level of major detoxifying enzymes, such as carboxylesterase (α-and β-) and SOD, significantly declined in both third and fourth instar larvae at the maximum dosage of My-It 1 × 105 conidia/mL. However, the level of glutathione S-transferase (GST) and cytochrome P-450 (CYP450) declined steadily when the sub-lethal dosage was increased and attained maximum reduction in the enzyme level at the dosage of My-It (1 × 105 conidia/mL). Correspondingly, the gut-histology and photomicrography results made evident that My-It (1 × 105 conidia/mL) heavily damaged the internal gut cells and external physiology of the dengue larvae compared to the control. Moreover, the non-target toxicity against the beneficial predator revealed that My-It at the maximum dosage (1 × 1020 conidia/mL) was found to be less toxic with <45% larval toxicity against Tx. splendens. Thus, the present toxicological research on Isaria tenuipes showed that it is target-specific and a potential agent for managing medically threatening arthropods. 2020-10-14T08:22:27Z 2020-10-14T08:22:27Z 2020-10-01 Journal 2309608X 2-s2.0-85092095621 10.3390/jof6040196 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85092095621&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/69954
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Medicine
spellingShingle Agricultural and Biological Sciences
Medicine
Sengodan Karthi
Prabhakaran Vasantha-Srinivasan
Raja Ganesan
Venkatachalam Ramasamy
Sengottayan Senthil-Nathan
Hanem F. Khater
Narayanaswamy Radhakrishnan
Kesavan Amala
Tae Jin Kim
Mohamed A. El-Sheikh
Patcharin Krutmuang
Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators
description © 2020 by the authors. Licensee MDPI, Basel, Switzerland. The present investigation aimed to determine the fungal toxicity of Isaria tenuipes (My-It) against the dengue mosquito vector Aedes aegypti L. and its non-target impact against the aquatic predator Toxorhynchites splendens. Lethal concentrations (LC50 and LC90) of My-It were observed in 2.27 and 2.93 log ppm dosages, respectively. The sub-lethal dosage (My-It-1 × 104 conidia/mL) displayed a significant oviposition deterrence index and also blocked the fecundity rate of dengue mosquitos in a dose-dependent manner. The level of major detoxifying enzymes, such as carboxylesterase (α-and β-) and SOD, significantly declined in both third and fourth instar larvae at the maximum dosage of My-It 1 × 105 conidia/mL. However, the level of glutathione S-transferase (GST) and cytochrome P-450 (CYP450) declined steadily when the sub-lethal dosage was increased and attained maximum reduction in the enzyme level at the dosage of My-It (1 × 105 conidia/mL). Correspondingly, the gut-histology and photomicrography results made evident that My-It (1 × 105 conidia/mL) heavily damaged the internal gut cells and external physiology of the dengue larvae compared to the control. Moreover, the non-target toxicity against the beneficial predator revealed that My-It at the maximum dosage (1 × 1020 conidia/mL) was found to be less toxic with <45% larval toxicity against Tx. splendens. Thus, the present toxicological research on Isaria tenuipes showed that it is target-specific and a potential agent for managing medically threatening arthropods.
format Journal
author Sengodan Karthi
Prabhakaran Vasantha-Srinivasan
Raja Ganesan
Venkatachalam Ramasamy
Sengottayan Senthil-Nathan
Hanem F. Khater
Narayanaswamy Radhakrishnan
Kesavan Amala
Tae Jin Kim
Mohamed A. El-Sheikh
Patcharin Krutmuang
author_facet Sengodan Karthi
Prabhakaran Vasantha-Srinivasan
Raja Ganesan
Venkatachalam Ramasamy
Sengottayan Senthil-Nathan
Hanem F. Khater
Narayanaswamy Radhakrishnan
Kesavan Amala
Tae Jin Kim
Mohamed A. El-Sheikh
Patcharin Krutmuang
author_sort Sengodan Karthi
title Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators
title_short Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators
title_full Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators
title_fullStr Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators
title_full_unstemmed Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators
title_sort target activity of isaria tenuipes (hypocreales: clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85092095621&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/69954
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