Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The present investigation aimed to determine the fungal toxicity of Isaria tenuipes (My-It) against the dengue mosquito vector Aedes aegypti L. and its non-target impact against the aquatic predator Toxorhynchites splendens. Lethal concentrat...
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th-cmuir.6653943832-699542020-10-14T08:40:54Z Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators Sengodan Karthi Prabhakaran Vasantha-Srinivasan Raja Ganesan Venkatachalam Ramasamy Sengottayan Senthil-Nathan Hanem F. Khater Narayanaswamy Radhakrishnan Kesavan Amala Tae Jin Kim Mohamed A. El-Sheikh Patcharin Krutmuang Agricultural and Biological Sciences Medicine © 2020 by the authors. Licensee MDPI, Basel, Switzerland. The present investigation aimed to determine the fungal toxicity of Isaria tenuipes (My-It) against the dengue mosquito vector Aedes aegypti L. and its non-target impact against the aquatic predator Toxorhynchites splendens. Lethal concentrations (LC50 and LC90) of My-It were observed in 2.27 and 2.93 log ppm dosages, respectively. The sub-lethal dosage (My-It-1 × 104 conidia/mL) displayed a significant oviposition deterrence index and also blocked the fecundity rate of dengue mosquitos in a dose-dependent manner. The level of major detoxifying enzymes, such as carboxylesterase (α-and β-) and SOD, significantly declined in both third and fourth instar larvae at the maximum dosage of My-It 1 × 105 conidia/mL. However, the level of glutathione S-transferase (GST) and cytochrome P-450 (CYP450) declined steadily when the sub-lethal dosage was increased and attained maximum reduction in the enzyme level at the dosage of My-It (1 × 105 conidia/mL). Correspondingly, the gut-histology and photomicrography results made evident that My-It (1 × 105 conidia/mL) heavily damaged the internal gut cells and external physiology of the dengue larvae compared to the control. Moreover, the non-target toxicity against the beneficial predator revealed that My-It at the maximum dosage (1 × 1020 conidia/mL) was found to be less toxic with <45% larval toxicity against Tx. splendens. Thus, the present toxicological research on Isaria tenuipes showed that it is target-specific and a potential agent for managing medically threatening arthropods. 2020-10-14T08:22:27Z 2020-10-14T08:22:27Z 2020-10-01 Journal 2309608X 2-s2.0-85092095621 10.3390/jof6040196 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85092095621&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/69954 |
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Agricultural and Biological Sciences Medicine Sengodan Karthi Prabhakaran Vasantha-Srinivasan Raja Ganesan Venkatachalam Ramasamy Sengottayan Senthil-Nathan Hanem F. Khater Narayanaswamy Radhakrishnan Kesavan Amala Tae Jin Kim Mohamed A. El-Sheikh Patcharin Krutmuang Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators |
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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The present investigation aimed to determine the fungal toxicity of Isaria tenuipes (My-It) against the dengue mosquito vector Aedes aegypti L. and its non-target impact against the aquatic predator Toxorhynchites splendens. Lethal concentrations (LC50 and LC90) of My-It were observed in 2.27 and 2.93 log ppm dosages, respectively. The sub-lethal dosage (My-It-1 × 104 conidia/mL) displayed a significant oviposition deterrence index and also blocked the fecundity rate of dengue mosquitos in a dose-dependent manner. The level of major detoxifying enzymes, such as carboxylesterase (α-and β-) and SOD, significantly declined in both third and fourth instar larvae at the maximum dosage of My-It 1 × 105 conidia/mL. However, the level of glutathione S-transferase (GST) and cytochrome P-450 (CYP450) declined steadily when the sub-lethal dosage was increased and attained maximum reduction in the enzyme level at the dosage of My-It (1 × 105 conidia/mL). Correspondingly, the gut-histology and photomicrography results made evident that My-It (1 × 105 conidia/mL) heavily damaged the internal gut cells and external physiology of the dengue larvae compared to the control. Moreover, the non-target toxicity against the beneficial predator revealed that My-It at the maximum dosage (1 × 1020 conidia/mL) was found to be less toxic with <45% larval toxicity against Tx. splendens. Thus, the present toxicological research on Isaria tenuipes showed that it is target-specific and a potential agent for managing medically threatening arthropods. |
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Sengodan Karthi Prabhakaran Vasantha-Srinivasan Raja Ganesan Venkatachalam Ramasamy Sengottayan Senthil-Nathan Hanem F. Khater Narayanaswamy Radhakrishnan Kesavan Amala Tae Jin Kim Mohamed A. El-Sheikh Patcharin Krutmuang |
author_facet |
Sengodan Karthi Prabhakaran Vasantha-Srinivasan Raja Ganesan Venkatachalam Ramasamy Sengottayan Senthil-Nathan Hanem F. Khater Narayanaswamy Radhakrishnan Kesavan Amala Tae Jin Kim Mohamed A. El-Sheikh Patcharin Krutmuang |
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Sengodan Karthi |
title |
Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators |
title_short |
Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators |
title_full |
Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators |
title_fullStr |
Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators |
title_full_unstemmed |
Target activity of isaria tenuipes (Hypocreales: Clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators |
title_sort |
target activity of isaria tenuipes (hypocreales: clavicipitaceae) fungal strains against dengue vector aedes aegypti (linn.) and its non-target activity against aquatic predators |
publishDate |
2020 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85092095621&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/69954 |
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1681752814283390976 |