Molecular and morphological evidence of sibling species in Anopheles baileyi Edwards (Diptera: Culicidae) in Bhutan and Thailand

© 2020 Elsevier B.V. This paper reports the results of a molecular and morphological study of Anopheles baileyi in Bhutan and Thailand. Phylogenetic analyses of ribosomal (ITS2) and mitochondrial DNA (COI) sequences revealed the presence of four genetically distinct clades, three in Bhutan (Clades I...

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Main Authors: Pradya Somboon, Thanari Phanitchakun, Rinzin Namgay, Tenzin Wangdi, Dechen Pemo, Ralph E. Harbach
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/69976
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spelling th-cmuir.6653943832-699762020-10-14T08:41:14Z Molecular and morphological evidence of sibling species in Anopheles baileyi Edwards (Diptera: Culicidae) in Bhutan and Thailand Pradya Somboon Thanari Phanitchakun Rinzin Namgay Tenzin Wangdi Dechen Pemo Ralph E. Harbach Agricultural and Biological Sciences Immunology and Microbiology Medicine © 2020 Elsevier B.V. This paper reports the results of a molecular and morphological study of Anopheles baileyi in Bhutan and Thailand. Phylogenetic analyses of ribosomal (ITS2) and mitochondrial DNA (COI) sequences revealed the presence of four genetically distinct clades, three in Bhutan (Clades I, II and III) and one in Thailand (Clade IV). Most of the larvae in the Bhutanese clades differed from those in the Thai clade in having seta 4-C branched, whereas it is single in the latter. The adults of each clade showed variation of wing markings and overlapping characters. The combination of characteristics of thoracic setae 1,2-P and abdominal seta 3-I was found to be useful for distinguishing the larvae. Pupae were inseparable. We provisionally recognize mosquitoes of Clades I, II, III and IV as members of a sibling species complex, the Baileyi Complex, denoted as species A, B, C and D, respectively. Species A is most likely the type form of An. baileyi s.s. because it was found adjacent to the type locality (Yatung, Tibet), and the others are unrecognized species. 2020-10-14T08:22:39Z 2020-10-14T08:22:39Z 2020-09-01 Journal 18736254 0001706X 2-s2.0-85085764635 10.1016/j.actatropica.2020.105549 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85085764635&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/69976
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Immunology and Microbiology
Medicine
spellingShingle Agricultural and Biological Sciences
Immunology and Microbiology
Medicine
Pradya Somboon
Thanari Phanitchakun
Rinzin Namgay
Tenzin Wangdi
Dechen Pemo
Ralph E. Harbach
Molecular and morphological evidence of sibling species in Anopheles baileyi Edwards (Diptera: Culicidae) in Bhutan and Thailand
description © 2020 Elsevier B.V. This paper reports the results of a molecular and morphological study of Anopheles baileyi in Bhutan and Thailand. Phylogenetic analyses of ribosomal (ITS2) and mitochondrial DNA (COI) sequences revealed the presence of four genetically distinct clades, three in Bhutan (Clades I, II and III) and one in Thailand (Clade IV). Most of the larvae in the Bhutanese clades differed from those in the Thai clade in having seta 4-C branched, whereas it is single in the latter. The adults of each clade showed variation of wing markings and overlapping characters. The combination of characteristics of thoracic setae 1,2-P and abdominal seta 3-I was found to be useful for distinguishing the larvae. Pupae were inseparable. We provisionally recognize mosquitoes of Clades I, II, III and IV as members of a sibling species complex, the Baileyi Complex, denoted as species A, B, C and D, respectively. Species A is most likely the type form of An. baileyi s.s. because it was found adjacent to the type locality (Yatung, Tibet), and the others are unrecognized species.
format Journal
author Pradya Somboon
Thanari Phanitchakun
Rinzin Namgay
Tenzin Wangdi
Dechen Pemo
Ralph E. Harbach
author_facet Pradya Somboon
Thanari Phanitchakun
Rinzin Namgay
Tenzin Wangdi
Dechen Pemo
Ralph E. Harbach
author_sort Pradya Somboon
title Molecular and morphological evidence of sibling species in Anopheles baileyi Edwards (Diptera: Culicidae) in Bhutan and Thailand
title_short Molecular and morphological evidence of sibling species in Anopheles baileyi Edwards (Diptera: Culicidae) in Bhutan and Thailand
title_full Molecular and morphological evidence of sibling species in Anopheles baileyi Edwards (Diptera: Culicidae) in Bhutan and Thailand
title_fullStr Molecular and morphological evidence of sibling species in Anopheles baileyi Edwards (Diptera: Culicidae) in Bhutan and Thailand
title_full_unstemmed Molecular and morphological evidence of sibling species in Anopheles baileyi Edwards (Diptera: Culicidae) in Bhutan and Thailand
title_sort molecular and morphological evidence of sibling species in anopheles baileyi edwards (diptera: culicidae) in bhutan and thailand
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85085764635&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/69976
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