Impact of sacbrood virus on larval microbiome of apis mellifera and apis cerana

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. In this study, we examined the impact of Sacbrood virus (SBV), the cause of larval honeybee (Apis mellifera) death, producing a liquefied a larva sac, on the gut bacterial communities on two larval honeybee species, Apis mellifera and Apis ce...

Full description

Saved in:
Bibliographic Details
Main Authors: Rujipas Yongsawas, Veeranan Chaimanee, Jeffery S. Pettis, Humberto Freire Boncristiani Junior, Dawn Lopez, Ammarin In-On, Panuwan Chantawannakul, Terd Disayathanoowat
Format: Journal
Published: 2020
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85087926908&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70022
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-70022
record_format dspace
spelling th-cmuir.6653943832-700222020-10-14T08:23:05Z Impact of sacbrood virus on larval microbiome of apis mellifera and apis cerana Rujipas Yongsawas Veeranan Chaimanee Jeffery S. Pettis Humberto Freire Boncristiani Junior Dawn Lopez Ammarin In-On Panuwan Chantawannakul Terd Disayathanoowat Agricultural and Biological Sciences © 2020 by the authors. Licensee MDPI, Basel, Switzerland. In this study, we examined the impact of Sacbrood virus (SBV), the cause of larval honeybee (Apis mellifera) death, producing a liquefied a larva sac, on the gut bacterial communities on two larval honeybee species, Apis mellifera and Apis cerana. SBV was added into a worker jelly food mixture and bee larvae were grafted into each of the treatment groups for 24 h before DNA/RNA extraction. Confirmation of SBV infection was achieved using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and visual symptomology. The 16S rDNA was sequenced by Illumina sequencing. The results showed the larvae were infected with SBV. The gut communities of infected A. cerana larvae exhibited a dramatic change compared with A. mellifera. In A. mellifera larvae, the Illumina sequencing revealed the proportion of Gilliamella, Snodgrassella and Fructobacillus was not significantly different, whereas in A. cerana, Gilliamella was significantly decreased (from 35.54% to 2.96%), however, with significant increase in Snodgrassella and Fructobacillus. The possibility of cross-infection should be further investigated. 2020-10-14T08:23:05Z 2020-10-14T08:23:05Z 2020-07-01 Journal 20754450 2-s2.0-85087926908 10.3390/insects11070439 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85087926908&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70022
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Rujipas Yongsawas
Veeranan Chaimanee
Jeffery S. Pettis
Humberto Freire Boncristiani Junior
Dawn Lopez
Ammarin In-On
Panuwan Chantawannakul
Terd Disayathanoowat
Impact of sacbrood virus on larval microbiome of apis mellifera and apis cerana
description © 2020 by the authors. Licensee MDPI, Basel, Switzerland. In this study, we examined the impact of Sacbrood virus (SBV), the cause of larval honeybee (Apis mellifera) death, producing a liquefied a larva sac, on the gut bacterial communities on two larval honeybee species, Apis mellifera and Apis cerana. SBV was added into a worker jelly food mixture and bee larvae were grafted into each of the treatment groups for 24 h before DNA/RNA extraction. Confirmation of SBV infection was achieved using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and visual symptomology. The 16S rDNA was sequenced by Illumina sequencing. The results showed the larvae were infected with SBV. The gut communities of infected A. cerana larvae exhibited a dramatic change compared with A. mellifera. In A. mellifera larvae, the Illumina sequencing revealed the proportion of Gilliamella, Snodgrassella and Fructobacillus was not significantly different, whereas in A. cerana, Gilliamella was significantly decreased (from 35.54% to 2.96%), however, with significant increase in Snodgrassella and Fructobacillus. The possibility of cross-infection should be further investigated.
format Journal
author Rujipas Yongsawas
Veeranan Chaimanee
Jeffery S. Pettis
Humberto Freire Boncristiani Junior
Dawn Lopez
Ammarin In-On
Panuwan Chantawannakul
Terd Disayathanoowat
author_facet Rujipas Yongsawas
Veeranan Chaimanee
Jeffery S. Pettis
Humberto Freire Boncristiani Junior
Dawn Lopez
Ammarin In-On
Panuwan Chantawannakul
Terd Disayathanoowat
author_sort Rujipas Yongsawas
title Impact of sacbrood virus on larval microbiome of apis mellifera and apis cerana
title_short Impact of sacbrood virus on larval microbiome of apis mellifera and apis cerana
title_full Impact of sacbrood virus on larval microbiome of apis mellifera and apis cerana
title_fullStr Impact of sacbrood virus on larval microbiome of apis mellifera and apis cerana
title_full_unstemmed Impact of sacbrood virus on larval microbiome of apis mellifera and apis cerana
title_sort impact of sacbrood virus on larval microbiome of apis mellifera and apis cerana
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85087926908&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70022
_version_ 1681752826834845696