Detection and molecular characterization of carbendazim-resistant Colletotrichum truncatum Isolates causing anthracnose of soybean in Thailand

© 2020 Blackwell Verlag GmbH A loss of fungicide efficacy, particularly for carbendazim, was noted in soybean fields in Thailand and was considered to be due to the development of Colletotrichum truncatum resistance. The carbendazim sensitivity of C. truncatum populations isolated from various soybe...

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Main Authors: Teeranai Poti, Kanyarat Mahawan, Ratchadawan Cheewangkoon, Hatthaya Arunothayanan, Kazuya Akimitsu, Sarunya Nalumpang
Format: Journal
Published: 2020
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spelling th-cmuir.6653943832-700442020-10-14T08:25:59Z Detection and molecular characterization of carbendazim-resistant Colletotrichum truncatum Isolates causing anthracnose of soybean in Thailand Teeranai Poti Kanyarat Mahawan Ratchadawan Cheewangkoon Hatthaya Arunothayanan Kazuya Akimitsu Sarunya Nalumpang Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology © 2020 Blackwell Verlag GmbH A loss of fungicide efficacy, particularly for carbendazim, was noted in soybean fields in Thailand and was considered to be due to the development of Colletotrichum truncatum resistance. The carbendazim sensitivity of C. truncatum populations isolated from various soybean fields in Thailand was thus evaluated with in vitro sensitivity assays and molecular characterization of mutations in the sequences of the ß2-tubulin (TUB2) gene that confer carbendazim resistance in the pathogen. Among 52 isolates, 46 isolates were classified as highly resistant (HR) to carbendazim (EC50 > 1,000 µg/ml). All HR isolates grew on PDA amended with carbendazim at 1,000 µg/ml. Six isolates were classified as carbendazim sensitive (S) (EC50 < 1 µg/ml). Mycelial growth on PDA amended with 1 µg/ml carbendazim was inhibited by over 50% compared with growth on PDA alone. When a partial TUB2 gene from the isolates was amplified and analysed using predicted amino acid sequences, an alteration from glutamic acid to alanine at codon 198 (E198A) was found in 45 HR isolates for which the EC50 was higher than 2000 µg/ml. This mutation resulted from a nucleotide substitution from adenine to cytosine (GAG → GCG). The other HR isolate, CtPhS_1, with EC50 of 1,127 µg/ml, had an alteration at codon 200 (F200Y) (TTC → TAC). 2020-10-14T08:23:22Z 2020-10-14T08:23:22Z 2020-05-01 Journal 14390434 09311785 2-s2.0-85083665192 10.1111/jph.12888 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083665192&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70044
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Teeranai Poti
Kanyarat Mahawan
Ratchadawan Cheewangkoon
Hatthaya Arunothayanan
Kazuya Akimitsu
Sarunya Nalumpang
Detection and molecular characterization of carbendazim-resistant Colletotrichum truncatum Isolates causing anthracnose of soybean in Thailand
description © 2020 Blackwell Verlag GmbH A loss of fungicide efficacy, particularly for carbendazim, was noted in soybean fields in Thailand and was considered to be due to the development of Colletotrichum truncatum resistance. The carbendazim sensitivity of C. truncatum populations isolated from various soybean fields in Thailand was thus evaluated with in vitro sensitivity assays and molecular characterization of mutations in the sequences of the ß2-tubulin (TUB2) gene that confer carbendazim resistance in the pathogen. Among 52 isolates, 46 isolates were classified as highly resistant (HR) to carbendazim (EC50 > 1,000 µg/ml). All HR isolates grew on PDA amended with carbendazim at 1,000 µg/ml. Six isolates were classified as carbendazim sensitive (S) (EC50 < 1 µg/ml). Mycelial growth on PDA amended with 1 µg/ml carbendazim was inhibited by over 50% compared with growth on PDA alone. When a partial TUB2 gene from the isolates was amplified and analysed using predicted amino acid sequences, an alteration from glutamic acid to alanine at codon 198 (E198A) was found in 45 HR isolates for which the EC50 was higher than 2000 µg/ml. This mutation resulted from a nucleotide substitution from adenine to cytosine (GAG → GCG). The other HR isolate, CtPhS_1, with EC50 of 1,127 µg/ml, had an alteration at codon 200 (F200Y) (TTC → TAC).
format Journal
author Teeranai Poti
Kanyarat Mahawan
Ratchadawan Cheewangkoon
Hatthaya Arunothayanan
Kazuya Akimitsu
Sarunya Nalumpang
author_facet Teeranai Poti
Kanyarat Mahawan
Ratchadawan Cheewangkoon
Hatthaya Arunothayanan
Kazuya Akimitsu
Sarunya Nalumpang
author_sort Teeranai Poti
title Detection and molecular characterization of carbendazim-resistant Colletotrichum truncatum Isolates causing anthracnose of soybean in Thailand
title_short Detection and molecular characterization of carbendazim-resistant Colletotrichum truncatum Isolates causing anthracnose of soybean in Thailand
title_full Detection and molecular characterization of carbendazim-resistant Colletotrichum truncatum Isolates causing anthracnose of soybean in Thailand
title_fullStr Detection and molecular characterization of carbendazim-resistant Colletotrichum truncatum Isolates causing anthracnose of soybean in Thailand
title_full_unstemmed Detection and molecular characterization of carbendazim-resistant Colletotrichum truncatum Isolates causing anthracnose of soybean in Thailand
title_sort detection and molecular characterization of carbendazim-resistant colletotrichum truncatum isolates causing anthracnose of soybean in thailand
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083665192&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70044
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