A polyphasic approach to delineate species in Bipolaris

© 2020, MUSHROOM RESEARCH FOUNDATION. Bipolaris species are important plant pathogens with a worldwide distribution in tropical and temperate environments. Species recognition in Bipolaris has been problematic due to a lack of molecular data from ex-type cultures, the use of few gene regions for spe...

Full description

Saved in:
Bibliographic Details
Main Authors: Chitrabhanu S. Bhunjun, Yang Dong, Ruvishika S. Jayawardena, Rajesh Jeewon, Chayanard Phukhamsakda, Digvijayini Bundhun, Kevin D. Hyde, Jun Sheng
Format: Journal
Published: 2020
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85085378513&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70047
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-70047
record_format dspace
spelling th-cmuir.6653943832-700472020-10-14T08:35:53Z A polyphasic approach to delineate species in Bipolaris Chitrabhanu S. Bhunjun Yang Dong Ruvishika S. Jayawardena Rajesh Jeewon Chayanard Phukhamsakda Digvijayini Bundhun Kevin D. Hyde Jun Sheng Agricultural and Biological Sciences Environmental Science © 2020, MUSHROOM RESEARCH FOUNDATION. Bipolaris species are important plant pathogens with a worldwide distribution in tropical and temperate environments. Species recognition in Bipolaris has been problematic due to a lack of molecular data from ex-type cultures, the use of few gene regions for species resolution and overlapping morphological characters. In this study, we evaluate the efficiency of different DNA barcodes in species delimitation in Bipolaris by phylogenetic analyses, Automatic Barcode Gap Discovery and Objective Clustering. GAPDH is determined to be the best single marker for the genus. These approaches are used to clarify the taxonomic placement of all sequences currently named as Bipolaris in GenBank based on ITS and GAPDH gene sequence data. In checking various publications, we found that the majority of new host records of fungal species published in the Plant Disease journal from 2010 to 2019 were based on BLAST searches of the ITS sequences and up to 82% of those records could be erroneous. Therefore, relying on BLAST searches from GenBank to name species is not recommended. Editorial boards of journals and reviewers of new record papers should be aware of this problem. In naming Bipolaris species, whether new or known, it is recommended to perform phylogenetic analyses based on GAPDH using the correct taxon sampling for accurate results and the species relationship should have reliable statistical support. At least two new species are represented by molecular data in GenBank and we provide an updated taxonomic revision of Bipolaris. We accept 45 species in Bipolaris and notes are provided for all the species including hosts and geographic distribution. 2020-10-14T08:23:24Z 2020-10-14T08:23:24Z 2020-05-01 Journal 18789129 15602745 2-s2.0-85085378513 10.1007/s13225-020-00446-6 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85085378513&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70047
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Environmental Science
spellingShingle Agricultural and Biological Sciences
Environmental Science
Chitrabhanu S. Bhunjun
Yang Dong
Ruvishika S. Jayawardena
Rajesh Jeewon
Chayanard Phukhamsakda
Digvijayini Bundhun
Kevin D. Hyde
Jun Sheng
A polyphasic approach to delineate species in Bipolaris
description © 2020, MUSHROOM RESEARCH FOUNDATION. Bipolaris species are important plant pathogens with a worldwide distribution in tropical and temperate environments. Species recognition in Bipolaris has been problematic due to a lack of molecular data from ex-type cultures, the use of few gene regions for species resolution and overlapping morphological characters. In this study, we evaluate the efficiency of different DNA barcodes in species delimitation in Bipolaris by phylogenetic analyses, Automatic Barcode Gap Discovery and Objective Clustering. GAPDH is determined to be the best single marker for the genus. These approaches are used to clarify the taxonomic placement of all sequences currently named as Bipolaris in GenBank based on ITS and GAPDH gene sequence data. In checking various publications, we found that the majority of new host records of fungal species published in the Plant Disease journal from 2010 to 2019 were based on BLAST searches of the ITS sequences and up to 82% of those records could be erroneous. Therefore, relying on BLAST searches from GenBank to name species is not recommended. Editorial boards of journals and reviewers of new record papers should be aware of this problem. In naming Bipolaris species, whether new or known, it is recommended to perform phylogenetic analyses based on GAPDH using the correct taxon sampling for accurate results and the species relationship should have reliable statistical support. At least two new species are represented by molecular data in GenBank and we provide an updated taxonomic revision of Bipolaris. We accept 45 species in Bipolaris and notes are provided for all the species including hosts and geographic distribution.
format Journal
author Chitrabhanu S. Bhunjun
Yang Dong
Ruvishika S. Jayawardena
Rajesh Jeewon
Chayanard Phukhamsakda
Digvijayini Bundhun
Kevin D. Hyde
Jun Sheng
author_facet Chitrabhanu S. Bhunjun
Yang Dong
Ruvishika S. Jayawardena
Rajesh Jeewon
Chayanard Phukhamsakda
Digvijayini Bundhun
Kevin D. Hyde
Jun Sheng
author_sort Chitrabhanu S. Bhunjun
title A polyphasic approach to delineate species in Bipolaris
title_short A polyphasic approach to delineate species in Bipolaris
title_full A polyphasic approach to delineate species in Bipolaris
title_fullStr A polyphasic approach to delineate species in Bipolaris
title_full_unstemmed A polyphasic approach to delineate species in Bipolaris
title_sort polyphasic approach to delineate species in bipolaris
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85085378513&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70047
_version_ 1681752831455920128