Melittin from apis florea venom as a promising anticancer agent
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was i...
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th-cmuir.6653943832-701962020-10-14T08:47:10Z Melittin from apis florea venom as a promising anticancer agent Sirikwan Sangboonruang Kuntida Kitidee Panuwan Chantawannakul Khajornsak Tragoolpua Yingmanee Tragoolpua Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine Pharmacology, Toxicology and Pharmaceutics © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells. In this study, Mel-AF exhibited considerable potential in the anti-proliferative action of A375 cells. Subsequently, the cellular mechanism of Mel-AF that induced cell death was investigated in terms of apoptosis. As a result, gene and protein expression levels, which indicated the activation of cytochrome-c release and caspase-9 expression, eventually triggered the release of the caspase-3 executioner upon Mel-AF. We then determined that apoptosis-mediated cell death was carried out through the intrinsic mitochondrial pathway. Moreover, advanced abilities, including cell motility and invasion, were significantly suppressed. Mel-AF manipulated the actin arrangement via the trapping of stress fibers that were found underneath the membrane, which resulted in the defective actin cytoskeleton organization. Consequently, the expression of EGFR, a binding protein to F-actin, was also found to be suppressed. This outcome strongly supports the effects of Mel-AF in the inhibition of progressive malignant activity through the disruption of actin cytoskeleton-EGFR interaction and the EGFR signaling system. Thus, the findings of our current study indicate the potential usefulness of Mel-AF in cancer treatments as an apoptosis inducer and a potential actin-targeting agent. 2020-10-14T08:25:27Z 2020-10-14T08:25:27Z 2020-08-01 Journal 20796382 2-s2.0-85089654342 10.3390/antibiotics9080517 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85089654342&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70196 |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine Pharmacology, Toxicology and Pharmaceutics Sirikwan Sangboonruang Kuntida Kitidee Panuwan Chantawannakul Khajornsak Tragoolpua Yingmanee Tragoolpua Melittin from apis florea venom as a promising anticancer agent |
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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells. In this study, Mel-AF exhibited considerable potential in the anti-proliferative action of A375 cells. Subsequently, the cellular mechanism of Mel-AF that induced cell death was investigated in terms of apoptosis. As a result, gene and protein expression levels, which indicated the activation of cytochrome-c release and caspase-9 expression, eventually triggered the release of the caspase-3 executioner upon Mel-AF. We then determined that apoptosis-mediated cell death was carried out through the intrinsic mitochondrial pathway. Moreover, advanced abilities, including cell motility and invasion, were significantly suppressed. Mel-AF manipulated the actin arrangement via the trapping of stress fibers that were found underneath the membrane, which resulted in the defective actin cytoskeleton organization. Consequently, the expression of EGFR, a binding protein to F-actin, was also found to be suppressed. This outcome strongly supports the effects of Mel-AF in the inhibition of progressive malignant activity through the disruption of actin cytoskeleton-EGFR interaction and the EGFR signaling system. Thus, the findings of our current study indicate the potential usefulness of Mel-AF in cancer treatments as an apoptosis inducer and a potential actin-targeting agent. |
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Sirikwan Sangboonruang Kuntida Kitidee Panuwan Chantawannakul Khajornsak Tragoolpua Yingmanee Tragoolpua |
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Sirikwan Sangboonruang Kuntida Kitidee Panuwan Chantawannakul Khajornsak Tragoolpua Yingmanee Tragoolpua |
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Sirikwan Sangboonruang |
title |
Melittin from apis florea venom as a promising anticancer agent |
title_short |
Melittin from apis florea venom as a promising anticancer agent |
title_full |
Melittin from apis florea venom as a promising anticancer agent |
title_fullStr |
Melittin from apis florea venom as a promising anticancer agent |
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Melittin from apis florea venom as a promising anticancer agent |
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melittin from apis florea venom as a promising anticancer agent |
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2020 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85089654342&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70196 |
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