Antibiotic-antiapoptotic dual function of clinacanthus nutans (Burm. F.) lindau leaf extracts against bovine mastitis

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Mastitis caused by bacterial infection has negative impacts on milk quality and animal health, and ultimately causes economic losses to the dairy industry worldwide. Gram-negative bacteria and their component lipopolysaccharide (LPS) can trig...

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Main Authors: Aussara Panya, Hataichanok Pundith, Supawadee Thongyim, Thida Kaewkod, Thararat Chitov, Sakunnee Bovonsombut, Yingmanee Tragoolpua
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Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/70209
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spelling th-cmuir.6653943832-702092020-10-14T08:47:11Z Antibiotic-antiapoptotic dual function of clinacanthus nutans (Burm. F.) lindau leaf extracts against bovine mastitis Aussara Panya Hataichanok Pundith Supawadee Thongyim Thida Kaewkod Thararat Chitov Sakunnee Bovonsombut Yingmanee Tragoolpua Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine Pharmacology, Toxicology and Pharmaceutics © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Mastitis caused by bacterial infection has negative impacts on milk quality and animal health, and ultimately causes economic losses to the dairy industry worldwide. Gram-negative bacteria and their component lipopolysaccharide (LPS) can trigger the inflammatory response of endothelial cells (ECs) and subsequently promote EC dysfunction or injury, which is a critical pathogenesis of mastitis-causing sepsis shock. To control the bacterial infection and to minimise the LPS negative effects on ECs, we thus aimed to identify the potential herb extracts that comprised antibacterial activity and protective ability to inhibit LPS-induced cell death. Extracts from seven types of herbs derived from antibacterial screening were investigated for their protective effects on LPS-stimulated bovine endothelial cell line. Clinacanthus nutans (Burm. f.) Lindau (C. nutans) extract appeared to be the most effective antiapoptotic extract against LPS stimulation. Treatment of C. nutans extract in LPS-stimulated cells significantly lowered apoptotic cell death through modulating pro-survival Bcl-2 and pro-apoptotic Bax expression. The investigation of bioactive compounds using solvent fractionation, HPLC, and LC-MS/MS analysis revealed glyceryl 1,3-disterate (C39H76O5), kaempferol 3-O-feruloyl-sophoroside 7-O-glucoside (C43H48O24), and hydroxypthioceranic acid (C46H92O3) as the candidate components. Our findings indicated that C. nutans extract has great potential to be further developed as an alternative therapeutic agent for mastitis treatment. 2020-10-14T08:25:37Z 2020-10-14T08:25:37Z 2020-07-01 Journal 20796382 2-s2.0-85088241566 10.3390/antibiotics9070429 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85088241566&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70209
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Pharmacology, Toxicology and Pharmaceutics
Aussara Panya
Hataichanok Pundith
Supawadee Thongyim
Thida Kaewkod
Thararat Chitov
Sakunnee Bovonsombut
Yingmanee Tragoolpua
Antibiotic-antiapoptotic dual function of clinacanthus nutans (Burm. F.) lindau leaf extracts against bovine mastitis
description © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Mastitis caused by bacterial infection has negative impacts on milk quality and animal health, and ultimately causes economic losses to the dairy industry worldwide. Gram-negative bacteria and their component lipopolysaccharide (LPS) can trigger the inflammatory response of endothelial cells (ECs) and subsequently promote EC dysfunction or injury, which is a critical pathogenesis of mastitis-causing sepsis shock. To control the bacterial infection and to minimise the LPS negative effects on ECs, we thus aimed to identify the potential herb extracts that comprised antibacterial activity and protective ability to inhibit LPS-induced cell death. Extracts from seven types of herbs derived from antibacterial screening were investigated for their protective effects on LPS-stimulated bovine endothelial cell line. Clinacanthus nutans (Burm. f.) Lindau (C. nutans) extract appeared to be the most effective antiapoptotic extract against LPS stimulation. Treatment of C. nutans extract in LPS-stimulated cells significantly lowered apoptotic cell death through modulating pro-survival Bcl-2 and pro-apoptotic Bax expression. The investigation of bioactive compounds using solvent fractionation, HPLC, and LC-MS/MS analysis revealed glyceryl 1,3-disterate (C39H76O5), kaempferol 3-O-feruloyl-sophoroside 7-O-glucoside (C43H48O24), and hydroxypthioceranic acid (C46H92O3) as the candidate components. Our findings indicated that C. nutans extract has great potential to be further developed as an alternative therapeutic agent for mastitis treatment.
format Journal
author Aussara Panya
Hataichanok Pundith
Supawadee Thongyim
Thida Kaewkod
Thararat Chitov
Sakunnee Bovonsombut
Yingmanee Tragoolpua
author_facet Aussara Panya
Hataichanok Pundith
Supawadee Thongyim
Thida Kaewkod
Thararat Chitov
Sakunnee Bovonsombut
Yingmanee Tragoolpua
author_sort Aussara Panya
title Antibiotic-antiapoptotic dual function of clinacanthus nutans (Burm. F.) lindau leaf extracts against bovine mastitis
title_short Antibiotic-antiapoptotic dual function of clinacanthus nutans (Burm. F.) lindau leaf extracts against bovine mastitis
title_full Antibiotic-antiapoptotic dual function of clinacanthus nutans (Burm. F.) lindau leaf extracts against bovine mastitis
title_fullStr Antibiotic-antiapoptotic dual function of clinacanthus nutans (Burm. F.) lindau leaf extracts against bovine mastitis
title_full_unstemmed Antibiotic-antiapoptotic dual function of clinacanthus nutans (Burm. F.) lindau leaf extracts against bovine mastitis
title_sort antibiotic-antiapoptotic dual function of clinacanthus nutans (burm. f.) lindau leaf extracts against bovine mastitis
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85088241566&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70209
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