Chemical constituents, antioxidant, Anti-MMPs, and anti-hyaluronidase activities of thunbergia laurifolia lindl. Leaf extracts for skin aging and skin damage prevention
© 2020 by the authors. This study aimed to investigate the potential usage of Thunbergia laurifolia Lindl. leaf extracts in the cosmetic industry. Matrix metalloproteinases (MMPs) and hyaluronidase inhibition of T. laurifolia leaf extracts, prepared using reflux extraction with deionized water (RE)...
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th-cmuir.6653943832-702442020-10-14T08:47:38Z Chemical constituents, antioxidant, Anti-MMPs, and anti-hyaluronidase activities of thunbergia laurifolia lindl. Leaf extracts for skin aging and skin damage prevention Wantida Chaiyana Sunee Chansakaow Nutjeera Intasai Kanokwan Kiattisin Kuan Han Lee Wei Chao Lin Shang Chian Lue Pimporn Leelapornpisid Biochemistry, Genetics and Molecular Biology Chemistry Pharmacology, Toxicology and Pharmaceutics © 2020 by the authors. This study aimed to investigate the potential usage of Thunbergia laurifolia Lindl. leaf extracts in the cosmetic industry. Matrix metalloproteinases (MMPs) and hyaluronidase inhibition of T. laurifolia leaf extracts, prepared using reflux extraction with deionized water (RE) and 80% v/v ethanol using Soxhlet’s apparatus (SE), were determined. Rosmarinic acid, phenolics, and flavonoids contents were determined using high-performance liquid chromatography, Folin–Ciocalteu, and aluminum chloride colorimetric assays, respectively. Antioxidant activities were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and linoleic acid-thiocyanate assays. MMP-1 inhibition was investigated using enzymatic and fluorescent reactions, whereas MMP-2, MMP-9, and hyaluronidase inhibition were investigated using gel electrophoresis. Cytotoxicity on human fibroblast cell line was also investigated. The results demonstrated that SE contained significantly higher content of rosmarinic acid (5.62% ± 0.01%) and flavonoids (417 ± 25 mg of quercetin/g of extract) but RE contained a significantly higher phenolics content (181 ± 1 mg of gallic acid/g of extract; p < 0.001). SE possessed higher lipid peroxidation inhibition but less DPPH• scavenging activity than RE. Both extracts possessed comparable hyaluronidase inhibition. SE was as potent an MMP-1 inhibitor as gallic acid (half maximal inhibitory concentration values were 12.0 ± 0.3 and 8.9 ± 0.4 mg/cm3, respectively). SE showed significantly higher MMP-2 and MMP-9 inhibition than RE (p < 0.05). Therefore, SE is a promising natural anti-ageing ingredient rich in rosmarinic acid and flavonoids with antioxidant, anti-hyaluronidase, and potent MMPs inhibitory effects that could be applied in the cosmetic industry. 2020-10-14T08:26:08Z 2020-10-14T08:26:08Z 2020-04-01 Journal 14203049 2-s2.0-85083776189 10.3390/molecules25081923 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083776189&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70244 |
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Biochemistry, Genetics and Molecular Biology Chemistry Pharmacology, Toxicology and Pharmaceutics Wantida Chaiyana Sunee Chansakaow Nutjeera Intasai Kanokwan Kiattisin Kuan Han Lee Wei Chao Lin Shang Chian Lue Pimporn Leelapornpisid Chemical constituents, antioxidant, Anti-MMPs, and anti-hyaluronidase activities of thunbergia laurifolia lindl. Leaf extracts for skin aging and skin damage prevention |
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© 2020 by the authors. This study aimed to investigate the potential usage of Thunbergia laurifolia Lindl. leaf extracts in the cosmetic industry. Matrix metalloproteinases (MMPs) and hyaluronidase inhibition of T. laurifolia leaf extracts, prepared using reflux extraction with deionized water (RE) and 80% v/v ethanol using Soxhlet’s apparatus (SE), were determined. Rosmarinic acid, phenolics, and flavonoids contents were determined using high-performance liquid chromatography, Folin–Ciocalteu, and aluminum chloride colorimetric assays, respectively. Antioxidant activities were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and linoleic acid-thiocyanate assays. MMP-1 inhibition was investigated using enzymatic and fluorescent reactions, whereas MMP-2, MMP-9, and hyaluronidase inhibition were investigated using gel electrophoresis. Cytotoxicity on human fibroblast cell line was also investigated. The results demonstrated that SE contained significantly higher content of rosmarinic acid (5.62% ± 0.01%) and flavonoids (417 ± 25 mg of quercetin/g of extract) but RE contained a significantly higher phenolics content (181 ± 1 mg of gallic acid/g of extract; p < 0.001). SE possessed higher lipid peroxidation inhibition but less DPPH• scavenging activity than RE. Both extracts possessed comparable hyaluronidase inhibition. SE was as potent an MMP-1 inhibitor as gallic acid (half maximal inhibitory concentration values were 12.0 ± 0.3 and 8.9 ± 0.4 mg/cm3, respectively). SE showed significantly higher MMP-2 and MMP-9 inhibition than RE (p < 0.05). Therefore, SE is a promising natural anti-ageing ingredient rich in rosmarinic acid and flavonoids with antioxidant, anti-hyaluronidase, and potent MMPs inhibitory effects that could be applied in the cosmetic industry. |
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Wantida Chaiyana Sunee Chansakaow Nutjeera Intasai Kanokwan Kiattisin Kuan Han Lee Wei Chao Lin Shang Chian Lue Pimporn Leelapornpisid |
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Wantida Chaiyana Sunee Chansakaow Nutjeera Intasai Kanokwan Kiattisin Kuan Han Lee Wei Chao Lin Shang Chian Lue Pimporn Leelapornpisid |
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Wantida Chaiyana |
title |
Chemical constituents, antioxidant, Anti-MMPs, and anti-hyaluronidase activities of thunbergia laurifolia lindl. Leaf extracts for skin aging and skin damage prevention |
title_short |
Chemical constituents, antioxidant, Anti-MMPs, and anti-hyaluronidase activities of thunbergia laurifolia lindl. Leaf extracts for skin aging and skin damage prevention |
title_full |
Chemical constituents, antioxidant, Anti-MMPs, and anti-hyaluronidase activities of thunbergia laurifolia lindl. Leaf extracts for skin aging and skin damage prevention |
title_fullStr |
Chemical constituents, antioxidant, Anti-MMPs, and anti-hyaluronidase activities of thunbergia laurifolia lindl. Leaf extracts for skin aging and skin damage prevention |
title_full_unstemmed |
Chemical constituents, antioxidant, Anti-MMPs, and anti-hyaluronidase activities of thunbergia laurifolia lindl. Leaf extracts for skin aging and skin damage prevention |
title_sort |
chemical constituents, antioxidant, anti-mmps, and anti-hyaluronidase activities of thunbergia laurifolia lindl. leaf extracts for skin aging and skin damage prevention |
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2020 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083776189&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70244 |
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