Sequential injection-differential pulse voltammetric immunosensor for hepatitis B surface antigen using the modified screen-printed carbon electrode

© 2020 Elsevier Ltd A label-free electrochemical immunosensor was developed for the determination of hepatitis B surface antigen (HBsAg), a biomarker of hepatitis B virus (HBV). It is based on the specific binding of antigen-antibody on the electrode surface, which hindered the oxidation of silver na...

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Main Authors: Jantima Upan, Philippe Banet, Pierre Henri Aubert, Kontad Ounnunkad, Jaroon Jakmunee
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/70332
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spelling th-cmuir.6653943832-703322020-10-14T08:28:49Z Sequential injection-differential pulse voltammetric immunosensor for hepatitis B surface antigen using the modified screen-printed carbon electrode Jantima Upan Philippe Banet Pierre Henri Aubert Kontad Ounnunkad Jaroon Jakmunee Chemical Engineering Chemistry © 2020 Elsevier Ltd A label-free electrochemical immunosensor was developed for the determination of hepatitis B surface antigen (HBsAg), a biomarker of hepatitis B virus (HBV). It is based on the specific binding of antigen-antibody on the electrode surface, which hindered the oxidation of silver nanoparticles (AgNPs) used as redox probe and hence reduced its oxidation current. The screen-printed carbon electrode (SPCE) was modified with carbon nanotube decorated with gold nanoparticles (AuNPs-CNT) and silver nanoparticles. The AuNPs provide good biocompatibility and large surface area for densely immobilizing the hepatitis B surface antibody (HBsAb) on the electrode, which helps to improve the signal. AgNPs act as a sensing probe in differential pulse voltammetric (DPV) detection. In addition, a sequential injection (SI) system was employed as an automated solution handling and minimizing chemical consumption. Characterization of the developed immunosensor by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) confirmed the successful modification/immobilization in various steps of immunosensor fabrication. The percentage of the decrease in oxidative peak current of AgNPs was directly proportional to the HBsAg concentration. The linear range of 1–40 ng mL−1 with a detection limit of 0.86 ng mL−1 HBsAg were achieved. The proposed method is economical, efficient, and useful for clinical analysis. 2020-10-14T08:27:42Z 2020-10-14T08:27:42Z 2020-07-20 Journal 00134686 2-s2.0-85084257271 10.1016/j.electacta.2020.136335 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85084257271&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70332
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Chemical Engineering
Chemistry
spellingShingle Chemical Engineering
Chemistry
Jantima Upan
Philippe Banet
Pierre Henri Aubert
Kontad Ounnunkad
Jaroon Jakmunee
Sequential injection-differential pulse voltammetric immunosensor for hepatitis B surface antigen using the modified screen-printed carbon electrode
description © 2020 Elsevier Ltd A label-free electrochemical immunosensor was developed for the determination of hepatitis B surface antigen (HBsAg), a biomarker of hepatitis B virus (HBV). It is based on the specific binding of antigen-antibody on the electrode surface, which hindered the oxidation of silver nanoparticles (AgNPs) used as redox probe and hence reduced its oxidation current. The screen-printed carbon electrode (SPCE) was modified with carbon nanotube decorated with gold nanoparticles (AuNPs-CNT) and silver nanoparticles. The AuNPs provide good biocompatibility and large surface area for densely immobilizing the hepatitis B surface antibody (HBsAb) on the electrode, which helps to improve the signal. AgNPs act as a sensing probe in differential pulse voltammetric (DPV) detection. In addition, a sequential injection (SI) system was employed as an automated solution handling and minimizing chemical consumption. Characterization of the developed immunosensor by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) confirmed the successful modification/immobilization in various steps of immunosensor fabrication. The percentage of the decrease in oxidative peak current of AgNPs was directly proportional to the HBsAg concentration. The linear range of 1–40 ng mL−1 with a detection limit of 0.86 ng mL−1 HBsAg were achieved. The proposed method is economical, efficient, and useful for clinical analysis.
format Journal
author Jantima Upan
Philippe Banet
Pierre Henri Aubert
Kontad Ounnunkad
Jaroon Jakmunee
author_facet Jantima Upan
Philippe Banet
Pierre Henri Aubert
Kontad Ounnunkad
Jaroon Jakmunee
author_sort Jantima Upan
title Sequential injection-differential pulse voltammetric immunosensor for hepatitis B surface antigen using the modified screen-printed carbon electrode
title_short Sequential injection-differential pulse voltammetric immunosensor for hepatitis B surface antigen using the modified screen-printed carbon electrode
title_full Sequential injection-differential pulse voltammetric immunosensor for hepatitis B surface antigen using the modified screen-printed carbon electrode
title_fullStr Sequential injection-differential pulse voltammetric immunosensor for hepatitis B surface antigen using the modified screen-printed carbon electrode
title_full_unstemmed Sequential injection-differential pulse voltammetric immunosensor for hepatitis B surface antigen using the modified screen-printed carbon electrode
title_sort sequential injection-differential pulse voltammetric immunosensor for hepatitis b surface antigen using the modified screen-printed carbon electrode
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85084257271&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70332
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