LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples

© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com. We developed a robust analytical method for quantification of malondialdehyde (MDA) in urine and serum samples using dansylhydrazine (DH) as a derivatizing re...

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Main Authors: Kostya Kartavenka, Parinya Panuwet, Volha Yakimavets, Churdsak Jaikang, Kanitarin Thipubon, Priya Esilda D'Souza, Dana Boyd Barr, P. Barry Ryan
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Published: 2020
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spelling th-cmuir.6653943832-703392020-10-14T08:47:26Z LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples Kostya Kartavenka Parinya Panuwet Volha Yakimavets Churdsak Jaikang Kanitarin Thipubon Priya Esilda D'Souza Dana Boyd Barr P. Barry Ryan Chemical Engineering Chemistry Environmental Science Pharmacology, Toxicology and Pharmaceutics © The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com. We developed a robust analytical method for quantification of malondialdehyde (MDA) in urine and serum samples using dansylhydrazine (DH) as a derivatizing reagent. The derivatization procedure was partially carried out using an autosampler injection program to minimize errors associated with the low-volume addition of reagents and was optimized to yield a stable hydrazone derivative of MDA and its labeled d2-MDA analogue. The target MDA-DH derivatives were separated on an Agilent Zorbax Eclipse Plus Phenyl-Hexyl (3.0 × 100 mm, 3.5 μm) column. The mass-to-charge ratios of the target derivatives [(M+H)+ of 302 and 304 for MDA-DH and d2-MDA-DH, respectively] were analyzed in single ion monitoring mode using a single quadrupole mass spectrometer operated under positive electrospray ionization. The method limits of quantification were 5.63 nM (or 0.405 ng/mL) for urine analysis and 5.68 nM (or 0.409 ng/mL) for serum analysis. The quantification range for urine analysis was 5.63-500 nM (0.405-36.0 ng/mL) while the quantification range for serum analysis was 5.68-341 nM (0.409-24.6 ng/mL). The method showed good relative recoveries (98-103%), good accuracies (92-98%), and acceptable precisions (relative standard deviations 1.8-7.3% for inter-day precision; 1.8-6.1% for intra-day precision) as observed from the repeat analysis of quality control samples prepared at different concentrations. The method was used to measure MDA in individual urine samples (n = 287) and de-identified archived serum samples (n = 22) to assess the overall performance of the method. The results demonstrated that our method is capable of measuring urinary and serum levels of MDA, allowing its future application in epidemiologic investigations. 2020-10-14T08:27:51Z 2020-10-14T08:27:51Z 2020-04-02 Journal 19452403 2-s2.0-85086884756 10.1093/jat/bkz112 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85086884756&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70339
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Chemical Engineering
Chemistry
Environmental Science
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Chemical Engineering
Chemistry
Environmental Science
Pharmacology, Toxicology and Pharmaceutics
Kostya Kartavenka
Parinya Panuwet
Volha Yakimavets
Churdsak Jaikang
Kanitarin Thipubon
Priya Esilda D'Souza
Dana Boyd Barr
P. Barry Ryan
LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples
description © The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com. We developed a robust analytical method for quantification of malondialdehyde (MDA) in urine and serum samples using dansylhydrazine (DH) as a derivatizing reagent. The derivatization procedure was partially carried out using an autosampler injection program to minimize errors associated with the low-volume addition of reagents and was optimized to yield a stable hydrazone derivative of MDA and its labeled d2-MDA analogue. The target MDA-DH derivatives were separated on an Agilent Zorbax Eclipse Plus Phenyl-Hexyl (3.0 × 100 mm, 3.5 μm) column. The mass-to-charge ratios of the target derivatives [(M+H)+ of 302 and 304 for MDA-DH and d2-MDA-DH, respectively] were analyzed in single ion monitoring mode using a single quadrupole mass spectrometer operated under positive electrospray ionization. The method limits of quantification were 5.63 nM (or 0.405 ng/mL) for urine analysis and 5.68 nM (or 0.409 ng/mL) for serum analysis. The quantification range for urine analysis was 5.63-500 nM (0.405-36.0 ng/mL) while the quantification range for serum analysis was 5.68-341 nM (0.409-24.6 ng/mL). The method showed good relative recoveries (98-103%), good accuracies (92-98%), and acceptable precisions (relative standard deviations 1.8-7.3% for inter-day precision; 1.8-6.1% for intra-day precision) as observed from the repeat analysis of quality control samples prepared at different concentrations. The method was used to measure MDA in individual urine samples (n = 287) and de-identified archived serum samples (n = 22) to assess the overall performance of the method. The results demonstrated that our method is capable of measuring urinary and serum levels of MDA, allowing its future application in epidemiologic investigations.
format Journal
author Kostya Kartavenka
Parinya Panuwet
Volha Yakimavets
Churdsak Jaikang
Kanitarin Thipubon
Priya Esilda D'Souza
Dana Boyd Barr
P. Barry Ryan
author_facet Kostya Kartavenka
Parinya Panuwet
Volha Yakimavets
Churdsak Jaikang
Kanitarin Thipubon
Priya Esilda D'Souza
Dana Boyd Barr
P. Barry Ryan
author_sort Kostya Kartavenka
title LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples
title_short LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples
title_full LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples
title_fullStr LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples
title_full_unstemmed LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples
title_sort lc-ms quantification of malondialdehyde-dansylhydrazine derivatives in urine and serum samples
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85086884756&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70339
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