A new spectrophotometric method based on peroxidase enzymatic reaction to determine tetracycline in pharmaceutical and water samples

© 2020, Iranian Chemical Society. An enzymatic method for cost-effective and reliable spectrophotometry was described based on the catalytic reaction of peroxidase enzyme to detect and determine tetracycline. Daikon (Raphanus sativus L.), a local vegetable, was exploited as a source of peroxidase en...

Full description

Saved in:
Bibliographic Details
Main Authors: Sam ang Supharoek, Kraingkrai Ponhong, Bordin Weerasuk, Watsaka Siriangkhawut, Kate Grudpan
Format: Journal
Published: 2020
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083767510&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70375
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-70375
record_format dspace
spelling th-cmuir.6653943832-703752020-10-14T08:28:37Z A new spectrophotometric method based on peroxidase enzymatic reaction to determine tetracycline in pharmaceutical and water samples Sam ang Supharoek Kraingkrai Ponhong Bordin Weerasuk Watsaka Siriangkhawut Kate Grudpan Chemistry © 2020, Iranian Chemical Society. An enzymatic method for cost-effective and reliable spectrophotometry was described based on the catalytic reaction of peroxidase enzyme to detect and determine tetracycline. Daikon (Raphanus sativus L.), a local vegetable, was exploited as a source of peroxidase enzyme, extracted by a simple method and utilized for tetracycline detection without any purification steps. A blue color product which absorbed maximal wavelength at 600 nm was observed when mixing tetracycline, 4-aminoantipyrine and hydrogen peroxide in the presence of daikon peroxidase under pH 7.5. Parameters influencing the proposed method as pH, concentration of hydrogen peroxide, concentration of 4-aminoantipyrine, volume of crude enzyme extract, incubation temperature and incubation time were investigated and optimized. Cloud-point extraction using Triton X-114 as surfactant was employed for preconcentration of the blue color product prior to spectrophotometric analysis. The calibration curve of standard tetracycline showed good linearity in the range 0.05–10 mg L−1 with linear regression of r2 = 0.9982. Limit of detection and limit of quantification for tetracycline by this analytical method were 0.02 and 0.10 mg L−1, respectively. Relative standard deviation was lower than 5%. Recovery was determined between 81.1–112.8 and 97.2–114.8% for pharmaceutical formulations and water samples, respectively. Results indicated that the developed method provided many advantages including a cost-effective, suitable and reliable procedure to detect tetracycline in pharmaceutical formulations and water samples. Results obtained were not significantly different from those achieved by HPLC–UV. 2020-10-14T08:28:37Z 2020-10-14T08:28:37Z 2020-09-01 Journal 17352428 1735207X 2-s2.0-85083767510 10.1007/s13738-020-01934-x https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083767510&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/70375
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Chemistry
spellingShingle Chemistry
Sam ang Supharoek
Kraingkrai Ponhong
Bordin Weerasuk
Watsaka Siriangkhawut
Kate Grudpan
A new spectrophotometric method based on peroxidase enzymatic reaction to determine tetracycline in pharmaceutical and water samples
description © 2020, Iranian Chemical Society. An enzymatic method for cost-effective and reliable spectrophotometry was described based on the catalytic reaction of peroxidase enzyme to detect and determine tetracycline. Daikon (Raphanus sativus L.), a local vegetable, was exploited as a source of peroxidase enzyme, extracted by a simple method and utilized for tetracycline detection without any purification steps. A blue color product which absorbed maximal wavelength at 600 nm was observed when mixing tetracycline, 4-aminoantipyrine and hydrogen peroxide in the presence of daikon peroxidase under pH 7.5. Parameters influencing the proposed method as pH, concentration of hydrogen peroxide, concentration of 4-aminoantipyrine, volume of crude enzyme extract, incubation temperature and incubation time were investigated and optimized. Cloud-point extraction using Triton X-114 as surfactant was employed for preconcentration of the blue color product prior to spectrophotometric analysis. The calibration curve of standard tetracycline showed good linearity in the range 0.05–10 mg L−1 with linear regression of r2 = 0.9982. Limit of detection and limit of quantification for tetracycline by this analytical method were 0.02 and 0.10 mg L−1, respectively. Relative standard deviation was lower than 5%. Recovery was determined between 81.1–112.8 and 97.2–114.8% for pharmaceutical formulations and water samples, respectively. Results indicated that the developed method provided many advantages including a cost-effective, suitable and reliable procedure to detect tetracycline in pharmaceutical formulations and water samples. Results obtained were not significantly different from those achieved by HPLC–UV.
format Journal
author Sam ang Supharoek
Kraingkrai Ponhong
Bordin Weerasuk
Watsaka Siriangkhawut
Kate Grudpan
author_facet Sam ang Supharoek
Kraingkrai Ponhong
Bordin Weerasuk
Watsaka Siriangkhawut
Kate Grudpan
author_sort Sam ang Supharoek
title A new spectrophotometric method based on peroxidase enzymatic reaction to determine tetracycline in pharmaceutical and water samples
title_short A new spectrophotometric method based on peroxidase enzymatic reaction to determine tetracycline in pharmaceutical and water samples
title_full A new spectrophotometric method based on peroxidase enzymatic reaction to determine tetracycline in pharmaceutical and water samples
title_fullStr A new spectrophotometric method based on peroxidase enzymatic reaction to determine tetracycline in pharmaceutical and water samples
title_full_unstemmed A new spectrophotometric method based on peroxidase enzymatic reaction to determine tetracycline in pharmaceutical and water samples
title_sort new spectrophotometric method based on peroxidase enzymatic reaction to determine tetracycline in pharmaceutical and water samples
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85083767510&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/70375
_version_ 1681752891326464000