Optimization of a thermostable lipase from Bacillus stearothermophilus p1: Overexpression, purification, and characterization
An expression library was generated from a partial NcoI and HindIII digest of genomic DNA from the thermophilic bacterium, Bacillus stearothermophilus P1. The DNA fragments were cloned into the expression vector pQE-60 and transformed into Escherichia coli M15[pREP4]. Sequence analysis of a lipase g...
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| Main Authors: | , , , , |
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| 格式: | Article |
| 語言: | English |
| 出版: |
2014
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| 在線閱讀: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0034864538&partnerID=40&md5=1e1dec56dfc50e437ae8d554db85ec32 http://www.ncbi.nlm.nih.gov/pubmed/11483000 http://cmuir.cmu.ac.th/handle/6653943832/7137 |
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| 機構: | Chiang Mai University |
| 語言: | English |