Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening

Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening

A new capillary electrophoresis method using immobilized cells as the stationary phase has been developed. The power of this method is demonstrated by the separation and identification of endothelin antagonists on a capillary column coated by the transfected Chinese hamster ovary (CHO) cells with ov...

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Main Authors: Yu H.-M., Tseng M-J., Fang J.-M., Phutrakul S., Chen S.-T.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-2342457068&partnerID=40&md5=cc141eb6c2bbef94205b993bb515822d
http://cmuir.cmu.ac.th/handle/6653943832/7211
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-72112014-08-30T03:51:42Z Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening Yu H.-M. Tseng M-J. Fang J.-M. Phutrakul S. Chen S.-T. A new capillary electrophoresis method using immobilized cells as the stationary phase has been developed. The power of this method is demonstrated by the separation and identification of endothelin antagonists on a capillary column coated by the transfected Chinese hamster ovary (CHO) cells with overexpressing endothelin receptors. The screening results are validated by functional assays suppressing the increase of intracellular calcium concentration induced by endothelin-1. Instead of making efforts in isolating protein receptors, the easily prepared whole-cell capillary column provides a superior tool on the basis of ligand/receptor affinity for a rapid screening of potent drug candidates from compound libraries. © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 2014-08-30T03:51:42Z 2014-08-30T03:51:42Z 2004 Article 01730835 15095445 ELCTD http://www.scopus.com/inward/record.url?eid=2-s2.0-2342457068&partnerID=40&md5=cc141eb6c2bbef94205b993bb515822d http://cmuir.cmu.ac.th/handle/6653943832/7211 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description A new capillary electrophoresis method using immobilized cells as the stationary phase has been developed. The power of this method is demonstrated by the separation and identification of endothelin antagonists on a capillary column coated by the transfected Chinese hamster ovary (CHO) cells with overexpressing endothelin receptors. The screening results are validated by functional assays suppressing the increase of intracellular calcium concentration induced by endothelin-1. Instead of making efforts in isolating protein receptors, the easily prepared whole-cell capillary column provides a superior tool on the basis of ligand/receptor affinity for a rapid screening of potent drug candidates from compound libraries. © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
format Article
author Yu H.-M.
Tseng M-J.
Fang J.-M.
Phutrakul S.
Chen S.-T.
spellingShingle Yu H.-M.
Tseng M-J.
Fang J.-M.
Phutrakul S.
Chen S.-T.
Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening
author_facet Yu H.-M.
Tseng M-J.
Fang J.-M.
Phutrakul S.
Chen S.-T.
author_sort Yu H.-M.
title Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening
title_short Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening
title_full Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening
title_fullStr Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening
title_full_unstemmed Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening
title_sort capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-2342457068&partnerID=40&md5=cc141eb6c2bbef94205b993bb515822d
http://cmuir.cmu.ac.th/handle/6653943832/7211
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