A competitive ELISA for quantifying serum CD147: Reduction of soluble cd147 levels in cancer patient sera

CD147/EMMPRIN (extracellular matrix metalloproteinase inducer) is a cell surface glycoprotein that displays increased expression in many cancers. It has been previously demonstrated to participate in cancer metastasis and progression. In this study we used an anti-CD147 monoclonal antibody and a rec...

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Main Authors: Moonsom S., Tayapiwatana C., Wongkham S., Kongtawelert P., Kasinrerk W.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-77749270826&partnerID=40&md5=9aeff321cfc8d1bec5055034a680484d
http://www.ncbi.nlm.nih.gov/pubmed/20199151
http://cmuir.cmu.ac.th/handle/6653943832/748
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spelling th-cmuir.6653943832-7482014-08-29T09:02:03Z A competitive ELISA for quantifying serum CD147: Reduction of soluble cd147 levels in cancer patient sera Moonsom S. Tayapiwatana C. Wongkham S. Kongtawelert P. Kasinrerk W. CD147/EMMPRIN (extracellular matrix metalloproteinase inducer) is a cell surface glycoprotein that displays increased expression in many cancers. It has been previously demonstrated to participate in cancer metastasis and progression. In this study we used an anti-CD147 monoclonal antibody and a recombinant CD147 protein generated in our laboratory to establish a competitive ELISA for quantifying serum CD147 levels. Unexpectedly, the CD147 level was highest in sera of normal subjects and significantly reduced in sera of cancer patients. There was no significant difference in serum CD147 level between benign, non-metastatic, and metastatic stages of cancers. In regard to liver diseases, the maximal CD147 level was observed in sera of patients with hepatitis and hepatocellular carcinoma, and significantly decreased in patients with liver cirrhosis and cholangiocarcinoma. Our results imply that there may be homeostasis of CD147 levels in sera under normal physiological conditions, while such a level is altered in cancer patients. © 2010, Mary Ann Liebert, Inc. 2014-08-29T09:02:03Z 2014-08-29T09:02:03Z 2010 Article 15540014 10.1089/hyb.2009.0096 20199151 HYBRD http://www.scopus.com/inward/record.url?eid=2-s2.0-77749270826&partnerID=40&md5=9aeff321cfc8d1bec5055034a680484d http://www.ncbi.nlm.nih.gov/pubmed/20199151 http://cmuir.cmu.ac.th/handle/6653943832/748 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description CD147/EMMPRIN (extracellular matrix metalloproteinase inducer) is a cell surface glycoprotein that displays increased expression in many cancers. It has been previously demonstrated to participate in cancer metastasis and progression. In this study we used an anti-CD147 monoclonal antibody and a recombinant CD147 protein generated in our laboratory to establish a competitive ELISA for quantifying serum CD147 levels. Unexpectedly, the CD147 level was highest in sera of normal subjects and significantly reduced in sera of cancer patients. There was no significant difference in serum CD147 level between benign, non-metastatic, and metastatic stages of cancers. In regard to liver diseases, the maximal CD147 level was observed in sera of patients with hepatitis and hepatocellular carcinoma, and significantly decreased in patients with liver cirrhosis and cholangiocarcinoma. Our results imply that there may be homeostasis of CD147 levels in sera under normal physiological conditions, while such a level is altered in cancer patients. © 2010, Mary Ann Liebert, Inc.
format Article
author Moonsom S.
Tayapiwatana C.
Wongkham S.
Kongtawelert P.
Kasinrerk W.
spellingShingle Moonsom S.
Tayapiwatana C.
Wongkham S.
Kongtawelert P.
Kasinrerk W.
A competitive ELISA for quantifying serum CD147: Reduction of soluble cd147 levels in cancer patient sera
author_facet Moonsom S.
Tayapiwatana C.
Wongkham S.
Kongtawelert P.
Kasinrerk W.
author_sort Moonsom S.
title A competitive ELISA for quantifying serum CD147: Reduction of soluble cd147 levels in cancer patient sera
title_short A competitive ELISA for quantifying serum CD147: Reduction of soluble cd147 levels in cancer patient sera
title_full A competitive ELISA for quantifying serum CD147: Reduction of soluble cd147 levels in cancer patient sera
title_fullStr A competitive ELISA for quantifying serum CD147: Reduction of soluble cd147 levels in cancer patient sera
title_full_unstemmed A competitive ELISA for quantifying serum CD147: Reduction of soluble cd147 levels in cancer patient sera
title_sort competitive elisa for quantifying serum cd147: reduction of soluble cd147 levels in cancer patient sera
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-77749270826&partnerID=40&md5=9aeff321cfc8d1bec5055034a680484d
http://www.ncbi.nlm.nih.gov/pubmed/20199151
http://cmuir.cmu.ac.th/handle/6653943832/748
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