Determination of ciprofloxacin and nalidixic acid resistance in Campylobacter jejuni with a fluorogenic polymerase chain reaction assay

A fluorogenic polymerase chain reaction assay for the gyrA gene was used to determine the frequency of a Thr-86 mutation in Campylobacter jejuni isolates from food animals and humans in northern Thailand and to investigate the correlation between this mutation and bacterial resistance to fluoroquino...

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Main Authors: Padungtod P., Kaneene J.B., Wilson D.L., Bell J., Linz J.E.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-0037314768&partnerID=40&md5=a5ac20a8fee7937281a249d424dcf9bc
http://cmuir.cmu.ac.th/handle/6653943832/7509
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-75092014-08-30T04:11:41Z Determination of ciprofloxacin and nalidixic acid resistance in Campylobacter jejuni with a fluorogenic polymerase chain reaction assay Padungtod P. Kaneene J.B. Wilson D.L. Bell J. Linz J.E. A fluorogenic polymerase chain reaction assay for the gyrA gene was used to determine the frequency of a Thr-86 mutation in Campylobacter jejuni isolates from food animals and humans in northern Thailand and to investigate the correlation between this mutation and bacterial resistance to fluoroquinolones. Eighty-four isolates of C. jejuni were used: 65 from healthy chickens on farms, 16 from chickens at the slaughterhouse, 1 from chicken meat at the market, and 1 from a healthy farm worker. The microbroth dilution technique was used for in vitro susceptibility testing. MIC breakpoints established by the National Antimicrobial Resistance Monitoring System were used to categorize the resistance of C. jejuni to ciprofloxacin and nalidixic acid. Sixty of the 84 C. jejuni isolates tested carried the Thr-86 mutation in the gyrA gene. All isolates with ciprofloxacin MICs of ≥2 mg/liter carried the mutation, and no isolates with nalidixic acid MICs of ≤16 mg/liter carried the Thr-86-to-Ile mutation. There was a very strong association between ciprofloxacin resistance and the presence of the mutation (kappa = 0.971, P < 0.01). The association between the presence of the Thr-86-to-Ile mutation and nalidixic acid resistance was weaker (kappa = 0.859; P ≤ 0.01). 2014-08-30T04:11:41Z 2014-08-30T04:11:41Z 2003 Article 0362028X 12597495 JFPRD http://www.scopus.com/inward/record.url?eid=2-s2.0-0037314768&partnerID=40&md5=a5ac20a8fee7937281a249d424dcf9bc http://cmuir.cmu.ac.th/handle/6653943832/7509 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description A fluorogenic polymerase chain reaction assay for the gyrA gene was used to determine the frequency of a Thr-86 mutation in Campylobacter jejuni isolates from food animals and humans in northern Thailand and to investigate the correlation between this mutation and bacterial resistance to fluoroquinolones. Eighty-four isolates of C. jejuni were used: 65 from healthy chickens on farms, 16 from chickens at the slaughterhouse, 1 from chicken meat at the market, and 1 from a healthy farm worker. The microbroth dilution technique was used for in vitro susceptibility testing. MIC breakpoints established by the National Antimicrobial Resistance Monitoring System were used to categorize the resistance of C. jejuni to ciprofloxacin and nalidixic acid. Sixty of the 84 C. jejuni isolates tested carried the Thr-86 mutation in the gyrA gene. All isolates with ciprofloxacin MICs of ≥2 mg/liter carried the mutation, and no isolates with nalidixic acid MICs of ≤16 mg/liter carried the Thr-86-to-Ile mutation. There was a very strong association between ciprofloxacin resistance and the presence of the mutation (kappa = 0.971, P < 0.01). The association between the presence of the Thr-86-to-Ile mutation and nalidixic acid resistance was weaker (kappa = 0.859; P ≤ 0.01).
format Article
author Padungtod P.
Kaneene J.B.
Wilson D.L.
Bell J.
Linz J.E.
spellingShingle Padungtod P.
Kaneene J.B.
Wilson D.L.
Bell J.
Linz J.E.
Determination of ciprofloxacin and nalidixic acid resistance in Campylobacter jejuni with a fluorogenic polymerase chain reaction assay
author_facet Padungtod P.
Kaneene J.B.
Wilson D.L.
Bell J.
Linz J.E.
author_sort Padungtod P.
title Determination of ciprofloxacin and nalidixic acid resistance in Campylobacter jejuni with a fluorogenic polymerase chain reaction assay
title_short Determination of ciprofloxacin and nalidixic acid resistance in Campylobacter jejuni with a fluorogenic polymerase chain reaction assay
title_full Determination of ciprofloxacin and nalidixic acid resistance in Campylobacter jejuni with a fluorogenic polymerase chain reaction assay
title_fullStr Determination of ciprofloxacin and nalidixic acid resistance in Campylobacter jejuni with a fluorogenic polymerase chain reaction assay
title_full_unstemmed Determination of ciprofloxacin and nalidixic acid resistance in Campylobacter jejuni with a fluorogenic polymerase chain reaction assay
title_sort determination of ciprofloxacin and nalidixic acid resistance in campylobacter jejuni with a fluorogenic polymerase chain reaction assay
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-0037314768&partnerID=40&md5=a5ac20a8fee7937281a249d424dcf9bc
http://cmuir.cmu.ac.th/handle/6653943832/7509
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