Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis
Molecular epidemiology analyses of the 36 clinical isolates of Pasteurella multocida from various avian hosts in Japan between 1976 to 2007 including 5 reference strains from the U.S.A., Taiwan and Indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (SE-AFL...
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th-cmuir.6653943832-75132014-08-30T04:11:41Z Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis Sthitmatee N. Kataoka Y. Sawada T. Molecular epidemiology analyses of the 36 clinical isolates of Pasteurella multocida from various avian hosts in Japan between 1976 to 2007 including 5 reference strains from the U.S.A., Taiwan and Indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (SE-AFLP) comparison with the classical ApaI-based pulsed-field gel electrophoresis (PFGE). As the results, SE-AFLP gave 21 profiles while PFGE gave 20 profiles. The Simpson's index of diversity analysis indicated that SE-AFLP gave a high discrimination power than PFGE. This concluded that SE-AFLP is a higher discrimination power than PFGE to differentiate avian P. multocida isolates in Japan. In addition, the genetical profiles suggested that there is the evolution of somatic serotype 3 strain in the indigenous host of Japan. 2014-08-30T04:11:41Z 2014-08-30T04:11:41Z 2010 Article 9167250 10.1292/jvms.10-0181 20644339 http://www.scopus.com/inward/record.url?eid=2-s2.0-78649798054&partnerID=40&md5=a24a10f5ee6ccd882b176095dc394818 http://cmuir.cmu.ac.th/handle/6653943832/7513 English |
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Molecular epidemiology analyses of the 36 clinical isolates of Pasteurella multocida from various avian hosts in Japan between 1976 to 2007 including 5 reference strains from the U.S.A., Taiwan and Indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (SE-AFLP) comparison with the classical ApaI-based pulsed-field gel electrophoresis (PFGE). As the results, SE-AFLP gave 21 profiles while PFGE gave 20 profiles. The Simpson's index of diversity analysis indicated that SE-AFLP gave a high discrimination power than PFGE. This concluded that SE-AFLP is a higher discrimination power than PFGE to differentiate avian P. multocida isolates in Japan. In addition, the genetical profiles suggested that there is the evolution of somatic serotype 3 strain in the indigenous host of Japan. |
| format |
Article |
| author |
Sthitmatee N. Kataoka Y. Sawada T. |
| spellingShingle |
Sthitmatee N. Kataoka Y. Sawada T. Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis |
| author_facet |
Sthitmatee N. Kataoka Y. Sawada T. |
| author_sort |
Sthitmatee N. |
| title |
Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis |
| title_short |
Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis |
| title_full |
Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis |
| title_fullStr |
Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis |
| title_full_unstemmed |
Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis |
| title_sort |
molecular epidemiology of japanese avian pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-78649798054&partnerID=40&md5=a24a10f5ee6ccd882b176095dc394818 http://cmuir.cmu.ac.th/handle/6653943832/7513 |
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1681420813715111936 |