Production of cloned Asian elephant embryos using an interspecies somatic cell nuclear transfer (iSCNT) technique

This study was carried out to investigate the feasibility of using interspecies somatic cell nuclear transfer (iSCNT) techniques in Asian elephants (Elephas maximus), using elephant fibroblasts as donor cells and rabbit oocytes as the recipient cytoplasts. Elephant fibroblasts were collected post-mo...

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Main Authors: Sathanawongs A., Jarujinda Y., Rojanasthien S., Oranratnachai A.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-77956936201&partnerID=40&md5=53b65111eb744d193fbacca4215958b3
http://cmuir.cmu.ac.th/handle/6653943832/7518
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-75182014-08-30T04:11:42Z Production of cloned Asian elephant embryos using an interspecies somatic cell nuclear transfer (iSCNT) technique Sathanawongs A. Jarujinda Y. Rojanasthien S. Oranratnachai A. This study was carried out to investigate the feasibility of using interspecies somatic cell nuclear transfer (iSCNT) techniques in Asian elephants (Elephas maximus), using elephant fibroblasts as donor cells and rabbit oocytes as the recipient cytoplasts. Elephant fibroblasts were collected post-mortem from the ear skin of a female Asian elephant and cultured in vitro. Monolayer fibroblasts were trypsinized and used for SCNT. The comparative study showed that the blastocyst rates from iSCNT were significantly lower (P<0.05) than for rabbit SCNT and rabbit parthenogenesis (19.4,56.7, and 70.3%, respectively). The study tried to improve the efficiency of iSCNT by using phy tohemagglutinin-P (PH A-P) to increase the fusion rate and nocodazole to synchronize donor cells in the Gl stage of the cell cycle. The fusion rate of elephant-rabbit couples treated with PHA-P was significantly increased (47.5%) compared with the non-treated group (26.2%; P<0.05). The cloned embryos could develop into blastocysts (27.8%), which was significantly (/'<0.05) higher than for the non-treated cells group (14.4%). By using this protocol, Asian elephant blastocysts can be produced by iSCNT of fibroblast cells into rabbit cytoplasts with the use of nocodazole and PHA-P. The blastocyst rate of cloned elephant embryos using rabbit cytoplasts was more than 25% and may have potential in further studies on elephant preservation. 2014-08-30T04:11:42Z 2014-08-30T04:11:42Z 2010 Article 755192 http://www.scopus.com/inward/record.url?eid=2-s2.0-77956936201&partnerID=40&md5=53b65111eb744d193fbacca4215958b3 http://cmuir.cmu.ac.th/handle/6653943832/7518 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description This study was carried out to investigate the feasibility of using interspecies somatic cell nuclear transfer (iSCNT) techniques in Asian elephants (Elephas maximus), using elephant fibroblasts as donor cells and rabbit oocytes as the recipient cytoplasts. Elephant fibroblasts were collected post-mortem from the ear skin of a female Asian elephant and cultured in vitro. Monolayer fibroblasts were trypsinized and used for SCNT. The comparative study showed that the blastocyst rates from iSCNT were significantly lower (P<0.05) than for rabbit SCNT and rabbit parthenogenesis (19.4,56.7, and 70.3%, respectively). The study tried to improve the efficiency of iSCNT by using phy tohemagglutinin-P (PH A-P) to increase the fusion rate and nocodazole to synchronize donor cells in the Gl stage of the cell cycle. The fusion rate of elephant-rabbit couples treated with PHA-P was significantly increased (47.5%) compared with the non-treated group (26.2%; P<0.05). The cloned embryos could develop into blastocysts (27.8%), which was significantly (/'<0.05) higher than for the non-treated cells group (14.4%). By using this protocol, Asian elephant blastocysts can be produced by iSCNT of fibroblast cells into rabbit cytoplasts with the use of nocodazole and PHA-P. The blastocyst rate of cloned elephant embryos using rabbit cytoplasts was more than 25% and may have potential in further studies on elephant preservation.
format Article
author Sathanawongs A.
Jarujinda Y.
Rojanasthien S.
Oranratnachai A.
spellingShingle Sathanawongs A.
Jarujinda Y.
Rojanasthien S.
Oranratnachai A.
Production of cloned Asian elephant embryos using an interspecies somatic cell nuclear transfer (iSCNT) technique
author_facet Sathanawongs A.
Jarujinda Y.
Rojanasthien S.
Oranratnachai A.
author_sort Sathanawongs A.
title Production of cloned Asian elephant embryos using an interspecies somatic cell nuclear transfer (iSCNT) technique
title_short Production of cloned Asian elephant embryos using an interspecies somatic cell nuclear transfer (iSCNT) technique
title_full Production of cloned Asian elephant embryos using an interspecies somatic cell nuclear transfer (iSCNT) technique
title_fullStr Production of cloned Asian elephant embryos using an interspecies somatic cell nuclear transfer (iSCNT) technique
title_full_unstemmed Production of cloned Asian elephant embryos using an interspecies somatic cell nuclear transfer (iSCNT) technique
title_sort production of cloned asian elephant embryos using an interspecies somatic cell nuclear transfer (iscnt) technique
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-77956936201&partnerID=40&md5=53b65111eb744d193fbacca4215958b3
http://cmuir.cmu.ac.th/handle/6653943832/7518
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