Production and characterization of anti-dengue capsid antibodies suggesting the N terminus region covering the first 20 amino acids of dengue virus capsid protein is predominantly immunogenic in mice
We produced monoclonal and polyclonal antibodies to the capsid (C) protein of dengue serotype 2 virus (DV2 C). First, a maltose-binding protein fused to DV2 C protein (MBP-C) was overproduced in E. coli. The affinity-purified MBP-C protein was cleaved by factor Xa protease to obtain a recombinant DV...
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Main Authors: | Puttikhunt C., Ong-ajchaowlerd P., Prommool T., Sangiambut S., Netsawang J., Limjindaporn T., Malasit P., Kasinrerk W. |
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Format: | Article |
Language: | English |
Published: |
2014
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Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-70349560295&partnerID=40&md5=1ef84007d5708763cd2b5feb14cc22fb http://cmuir.cmu.ac.th/handle/6653943832/770 |
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Institution: | Chiang Mai University |
Language: | English |
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