Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses
Background: CD99, a leukocyte surface glycoprotein, is broadly expressed in many cell types. On the cell surface, CD99 is expressed as two distinct isoforms, a long form and a short form. CD99 has been demonstrated to play a key role in several biological processes, including the regulation of T cel...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
2014
|
Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-80051586103&partnerID=40&md5=f497ddcead336363bd40b2cc9bd88aa2 http://www.ncbi.nlm.nih.gov/pubmed/21838920 http://cmuir.cmu.ac.th/handle/6653943832/796 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
Language: | English |
id |
th-cmuir.6653943832-796 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-7962014-08-29T09:02:08Z Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses Pata S. Otahal P. Brdikca T. Laopajon W. Mahasongkram K. Kasinrerk W. Background: CD99, a leukocyte surface glycoprotein, is broadly expressed in many cell types. On the cell surface, CD99 is expressed as two distinct isoforms, a long form and a short form. CD99 has been demonstrated to play a key role in several biological processes, including the regulation of T cell activation. However, the molecular mechanisms by which CD99 participates in such processes are unclear. As CD99 contains a short cytoplasmic tail, it is unlikely that CD99 itself takes part in its multi-functions. Association of CD99 with other membrane proteins has been suggested to be necessary for exerting its functions. Results: In this study, we analyzed the association of CD99 with other cell surface molecules involved in T cell activation. We demonstrate the association of MHC class I, MHC class II and tetraspanin CD81 with CD99 molecules on the cell surface. Association of CD99 with its partners was observed for both isoforms. In addition, we determined that CD99 is a lipid raft-associated membrane protein and is recruited into the immunologic synapse during T cell activation. The implication of CD99 on T cell activation was investigated. Inhibition of anti-CD3 induced T cell proliferation by an anti-CD99 monoclonal antibody was observed. Conclusions: We provide evidence that CD99 directly interact and form the complex with the MHC class I and II, and tetraspanin CD81, and is functionally linked to the formation of the immunologic synapse. Upon T cell activation, CD99 engagement can inhibit T cell proliferation. We speculate that the CD99-MHC-CD81 complex is a tetraspanin web that plays an important role in T cell activation. © 2011 Kasinrerk et al; licensee BioMed Central Ltd. 2014-08-29T09:02:08Z 2014-08-29T09:02:08Z 2011 Article 17560500 10.1186/1756-0500-4-293 http://www.scopus.com/inward/record.url?eid=2-s2.0-80051586103&partnerID=40&md5=f497ddcead336363bd40b2cc9bd88aa2 http://www.ncbi.nlm.nih.gov/pubmed/21838920 http://cmuir.cmu.ac.th/handle/6653943832/796 English |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
language |
English |
description |
Background: CD99, a leukocyte surface glycoprotein, is broadly expressed in many cell types. On the cell surface, CD99 is expressed as two distinct isoforms, a long form and a short form. CD99 has been demonstrated to play a key role in several biological processes, including the regulation of T cell activation. However, the molecular mechanisms by which CD99 participates in such processes are unclear. As CD99 contains a short cytoplasmic tail, it is unlikely that CD99 itself takes part in its multi-functions. Association of CD99 with other membrane proteins has been suggested to be necessary for exerting its functions. Results: In this study, we analyzed the association of CD99 with other cell surface molecules involved in T cell activation. We demonstrate the association of MHC class I, MHC class II and tetraspanin CD81 with CD99 molecules on the cell surface. Association of CD99 with its partners was observed for both isoforms. In addition, we determined that CD99 is a lipid raft-associated membrane protein and is recruited into the immunologic synapse during T cell activation. The implication of CD99 on T cell activation was investigated. Inhibition of anti-CD3 induced T cell proliferation by an anti-CD99 monoclonal antibody was observed. Conclusions: We provide evidence that CD99 directly interact and form the complex with the MHC class I and II, and tetraspanin CD81, and is functionally linked to the formation of the immunologic synapse. Upon T cell activation, CD99 engagement can inhibit T cell proliferation. We speculate that the CD99-MHC-CD81 complex is a tetraspanin web that plays an important role in T cell activation. © 2011 Kasinrerk et al; licensee BioMed Central Ltd. |
format |
Article |
author |
Pata S. Otahal P. Brdikca T. Laopajon W. Mahasongkram K. Kasinrerk W. |
spellingShingle |
Pata S. Otahal P. Brdikca T. Laopajon W. Mahasongkram K. Kasinrerk W. Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses |
author_facet |
Pata S. Otahal P. Brdikca T. Laopajon W. Mahasongkram K. Kasinrerk W. |
author_sort |
Pata S. |
title |
Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses |
title_short |
Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses |
title_full |
Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses |
title_fullStr |
Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses |
title_full_unstemmed |
Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses |
title_sort |
association of cd99 short and long forms with mhc class i, mhc class ii and tetraspanin cd81 and recruitment into immunological synapses |
publishDate |
2014 |
url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-80051586103&partnerID=40&md5=f497ddcead336363bd40b2cc9bd88aa2 http://www.ncbi.nlm.nih.gov/pubmed/21838920 http://cmuir.cmu.ac.th/handle/6653943832/796 |
_version_ |
1681419550591025152 |