A novel approach using streptavidin magnetic bead-sorted in vivo biotinylated survivin for monoclonal antibody production
One major obstacle in antibody production is the lack of highly purified immunogen. In this study, we describe an alternative strategy to circumvent this problem. A nucleotide sequence encoding a full-length of human survivin was cloned into pAK400cb. After transforming into an E. coli Origami B str...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
2014
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Online Access: | http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/811 |
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Institution: | Chiang Mai University |
Language: | English |