A novel approach using streptavidin magnetic bead-sorted in vivo biotinylated survivin for monoclonal antibody production

• المؤلفون الرئيسيون: Tayapiwatana C., Chotpadiwetkul R., Kasinrerk W.
• التنسيق: مقال
• اللغة: English
• منشور في: 2014
• الوصول للمادة أونلاين: http://www.ncbi.nlm.nih.gov/pubmed/3502482; http://cmuir.cmu.ac.th/handle/6653943832/811
• المؤسسة: Chiang Mai University

One major obstacle in antibody production is the lack of highly purified immunogen. In this study, we describe an alternative strategy to circumvent this problem. A nucleotide sequence encoding a full-length of human survivin was cloned into pAK400cb. After transforming into an E. coli Origami B str...