Agar plating technique for enumeration of IL-2 producing cells in human peripheral blood mononuclear leukocytes
An agar plating technique for detection and enumeration of IL-2 producing cells from human peripheral blood mononuclear leukocytes (PBML) has been developed. This method is based on the principle that PHA-stimulated PBML, as effector cells, secrete interleukin 2 (IL-2) into soft agar containing mous...
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Main Authors: | Hirunpetcharat C., Kasinrerk W., Makonkawkeyoon S. |
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Format: | In Vitro |
Language: | English |
Published: |
2014
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Online Access: | http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/843 |
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Institution: | Chiang Mai University |
Language: | English |
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