Agar plating technique for enumeration of IL-2 producing cells in human peripheral blood mononuclear leukocytes

• المؤلفون الرئيسيون: Hirunpetcharat C., Kasinrerk W., Makonkawkeyoon S.
• التنسيق: In Vitro
• اللغة: English
• منشور في: 2014
• الوصول للمادة أونلاين: http://www.ncbi.nlm.nih.gov/pubmed/3502482; http://cmuir.cmu.ac.th/handle/6653943832/843

An agar plating technique for detection and enumeration of IL-2 producing cells from human peripheral blood mononuclear leukocytes (PBML) has been developed. This method is based on the principle that PHA-stimulated PBML, as effector cells, secrete interleukin 2 (IL-2) into soft agar containing mous...