Enumeration of interleukin 2-producing cells from rat spleen

A method for the enumeration of IL2-producing cells from rat spleen has been developed. Rat spleen cells were stimulated with concanavalin A (Con A), washed, then mixed with IL2-dependent cells (3 day Con A blasts) and plated in soft agar. Clusters of IL2-dependent cells formed around IL2-producing...

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Bibliographic Details
Main Authors: Makonkawkeyoon S., Hirunpetcharat C., Kasinrerk W., Vithayasai V.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/844
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Institution: Chiang Mai University
Language: English

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