Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus
Upregulation of interleukin-10 (IL-10) expression has been suggested to be the mechanism by which the porcine reproductive and respiratory syndrome virus (PRRSV) suppresses the innate and adaptive immune response in infected pigs. In this study we evaluated the potential of phosphorothioate-modified...
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th-cmuir.6653943832-8592014-08-29T09:02:15Z Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus Charerntantanakul W. Kasinrerk W. Upregulation of interleukin-10 (IL-10) expression has been suggested to be the mechanism by which the porcine reproductive and respiratory syndrome virus (PRRSV) suppresses the innate and adaptive immune response in infected pigs. In this study we evaluated the potential of phosphorothioate-modified IL-10 antisense oligodeoxynucleotide specific to the translation initiation region of porcine IL-10 mRNA (IL-10AS) in enhancing proinflammatory cytokine responses to PRRSV. Naïve peripheral blood mononuclear cells from eight PRRSV-seronegative pigs were transfected with IL-10AS in vitro prior to PRRSV inoculation and phorbol 12-myristate 13-acetate plus ionomycin or concanavalin A stimulation. The effects of IL-10AS on mRNA expression of IL-10, interferon-γ (IFN-γ), IFN-α, tumor necrosis factor-α (TNF-α), IL-2, and IL-4 were tested by real-time PCR. The percentages of IFN-γ-producing T-cell subsets were determined by flow cytometry. Compared to the controls, the levels of IL-10 and IL-2 mRNA were significantly reduced, while those of IFN-γ mRNA were increased, and TNF-α, IFN-α, and IL-4 mRNA were unchanged. An increase in the percentage of the IFN-γ+ population was also observed in lymphocytes and CD8β+ T cells. Our results suggest that IL-10AS has the potential to enhance proinflammatory cytokine responses to PRRSV infection. Copyright 2010, Mary Ann Liebert, Inc. 2014-08-29T09:02:15Z 2014-08-29T09:02:15Z 2010 Article 8828245 10.1089/vim.2009.0066 20712487 VIIME http://www.scopus.com/inward/record.url?eid=2-s2.0-77955915518&partnerID=40&md5=bd66ac996697be62820c333d06b3462a http://cmuir.cmu.ac.th/handle/6653943832/859 English |
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Upregulation of interleukin-10 (IL-10) expression has been suggested to be the mechanism by which the porcine reproductive and respiratory syndrome virus (PRRSV) suppresses the innate and adaptive immune response in infected pigs. In this study we evaluated the potential of phosphorothioate-modified IL-10 antisense oligodeoxynucleotide specific to the translation initiation region of porcine IL-10 mRNA (IL-10AS) in enhancing proinflammatory cytokine responses to PRRSV. Naïve peripheral blood mononuclear cells from eight PRRSV-seronegative pigs were transfected with IL-10AS in vitro prior to PRRSV inoculation and phorbol 12-myristate 13-acetate plus ionomycin or concanavalin A stimulation. The effects of IL-10AS on mRNA expression of IL-10, interferon-γ (IFN-γ), IFN-α, tumor necrosis factor-α (TNF-α), IL-2, and IL-4 were tested by real-time PCR. The percentages of IFN-γ-producing T-cell subsets were determined by flow cytometry. Compared to the controls, the levels of IL-10 and IL-2 mRNA were significantly reduced, while those of IFN-γ mRNA were increased, and TNF-α, IFN-α, and IL-4 mRNA were unchanged. An increase in the percentage of the IFN-γ+ population was also observed in lymphocytes and CD8β+ T cells. Our results suggest that IL-10AS has the potential to enhance proinflammatory cytokine responses to PRRSV infection. Copyright 2010, Mary Ann Liebert, Inc. |
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Charerntantanakul W. Kasinrerk W. |
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Charerntantanakul W. Kasinrerk W. Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus |
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Charerntantanakul W. Kasinrerk W. |
author_sort |
Charerntantanakul W. |
title |
Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus |
title_short |
Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus |
title_full |
Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus |
title_fullStr |
Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus |
title_full_unstemmed |
Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus |
title_sort |
interleukin-10 antisense oligodeoxynucleotide suppresses il-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus |
publishDate |
2014 |
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http://www.scopus.com/inward/record.url?eid=2-s2.0-77955915518&partnerID=40&md5=bd66ac996697be62820c333d06b3462a http://cmuir.cmu.ac.th/handle/6653943832/859 |
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