Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells
Wilms' tumor (WT1) gene overexpresses in leukemic cells which is alternatively spliced at two sites, yielding four isoforms: WT1 (+/+), (+/-), (-/+), and (-/-). Curcumin is one of major active components of the spice turmeric, widely known as anticancer. This study investigated the effects and...
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th-cmuir.6653943832-8732014-08-29T09:02:16Z Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells Semsri S. Anuchapreeda S. Intasai N. Jomgeow T. Tima S. Sweeney C. Limtrakul P. Wilms' tumor (WT1) gene overexpresses in leukemic cells which is alternatively spliced at two sites, yielding four isoforms: WT1 (+/+), (+/-), (-/+), and (-/-). Curcumin is one of major active components of the spice turmeric, widely known as anticancer. This study investigated the effects and inhibitory mechanism of pure curcumin on WT1 isoform-transfected U937 cells. WT1 transfected U937 cells were initially treated for 24 h, with 10 μM pure curcumin. Pure curcumin exhibited a strong inhibitory effect on WT1 (+/+) mRNA level detected by real time PCR. Treatment of WT1 transfected U937 cells with non-cytotoxic doses (10, 15, and 17 μM) of pure curcumin decreased WT1 protein levels in a dose dependent manner. Pure curcumin at the concentration of 15 μM significantly decreased the protein levels of the WT (+/+) isoform in a time-dependent manner. It also decreased exogenous WT1 (+/+) protein half-life. WT1 protein expression was inhibited by protein kinase C inhibitor (GF109203x) suggesting that pure curcumin decreased exogenous WT1 (+/+) expression in transfected U937 cells via protein kinase C during post-translational processing. ©2011 Academic Journals. 2014-08-29T09:02:16Z 2014-08-29T09:02:16Z 2011 Article 19960816 10.5897/AJPP11.149 http://www.scopus.com/inward/record.url?eid=2-s2.0-80655146486&partnerID=40&md5=533ab12c4070293e41f60994352725ed http://cmuir.cmu.ac.th/handle/6653943832/873 English |
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Wilms' tumor (WT1) gene overexpresses in leukemic cells which is alternatively spliced at two sites, yielding four isoforms: WT1 (+/+), (+/-), (-/+), and (-/-). Curcumin is one of major active components of the spice turmeric, widely known as anticancer. This study investigated the effects and inhibitory mechanism of pure curcumin on WT1 isoform-transfected U937 cells. WT1 transfected U937 cells were initially treated for 24 h, with 10 μM pure curcumin. Pure curcumin exhibited a strong inhibitory effect on WT1 (+/+) mRNA level detected by real time PCR. Treatment of WT1 transfected U937 cells with non-cytotoxic doses (10, 15, and 17 μM) of pure curcumin decreased WT1 protein levels in a dose dependent manner. Pure curcumin at the concentration of 15 μM significantly decreased the protein levels of the WT (+/+) isoform in a time-dependent manner. It also decreased exogenous WT1 (+/+) protein half-life. WT1 protein expression was inhibited by protein kinase C inhibitor (GF109203x) suggesting that pure curcumin decreased exogenous WT1 (+/+) expression in transfected U937 cells via protein kinase C during post-translational processing. ©2011 Academic Journals. |
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Semsri S. Anuchapreeda S. Intasai N. Jomgeow T. Tima S. Sweeney C. Limtrakul P. |
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Semsri S. Anuchapreeda S. Intasai N. Jomgeow T. Tima S. Sweeney C. Limtrakul P. Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells |
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Semsri S. Anuchapreeda S. Intasai N. Jomgeow T. Tima S. Sweeney C. Limtrakul P. |
author_sort |
Semsri S. |
title |
Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells |
title_short |
Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells |
title_full |
Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells |
title_fullStr |
Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells |
title_full_unstemmed |
Pure curcumin inhibits exogenous Wilms' tumor (WT1) (+/+) isoform protein via degradation pathway and protein kinase C in transfected U937 cells |
title_sort |
pure curcumin inhibits exogenous wilms' tumor (wt1) (+/+) isoform protein via degradation pathway and protein kinase c in transfected u937 cells |
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2014 |
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http://www.scopus.com/inward/record.url?eid=2-s2.0-80655146486&partnerID=40&md5=533ab12c4070293e41f60994352725ed http://cmuir.cmu.ac.th/handle/6653943832/873 |
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