A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion

A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion

Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies...

Full description

Saved in:
Bibliographic Details
Main Authors: Pata S., Khummuang S., Pornprasert S., Tatu T., Kasinrerk W.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84890324046&partnerID=40&md5=86c8ae6d1a34ca37cada856aee1980f4
http://cmuir.cmu.ac.th/handle/6653943832/933
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-933
record_format dspace
spelling th-cmuir.6653943832-9332014-08-29T09:02:21Z A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion Pata S. Khummuang S. Pornprasert S. Tatu T. Kasinrerk W. Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies specific to the ζ-globin chain without cross reaction with other globin chains. A simple and sensitive ELISA was developed by using poly-l-lysine to increase the protein binding to the ELISA plate. The developed poly-l-lysine pre-coated ELISA has a very high sensitivity (100%) and specificity (97%) for detection of carriers of α-thalassemia-1 with Southeast Asian-type deletion. © 2014 Copyright Taylor and Francis Group, LLC. 2014-08-29T09:02:21Z 2014-08-29T09:02:21Z 2014 Article 15321819 10.1080/15321819.2013.838963 JIIOA http://www.scopus.com/inward/record.url?eid=2-s2.0-84890324046&partnerID=40&md5=86c8ae6d1a34ca37cada856aee1980f4 http://cmuir.cmu.ac.th/handle/6653943832/933 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies specific to the ζ-globin chain without cross reaction with other globin chains. A simple and sensitive ELISA was developed by using poly-l-lysine to increase the protein binding to the ELISA plate. The developed poly-l-lysine pre-coated ELISA has a very high sensitivity (100%) and specificity (97%) for detection of carriers of α-thalassemia-1 with Southeast Asian-type deletion. © 2014 Copyright Taylor and Francis Group, LLC.
format Article
author Pata S.
Khummuang S.
Pornprasert S.
Tatu T.
Kasinrerk W.
spellingShingle Pata S.
Khummuang S.
Pornprasert S.
Tatu T.
Kasinrerk W.
A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
author_facet Pata S.
Khummuang S.
Pornprasert S.
Tatu T.
Kasinrerk W.
author_sort Pata S.
title A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_short A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_full A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_fullStr A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_full_unstemmed A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
title_sort simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84890324046&partnerID=40&md5=86c8ae6d1a34ca37cada856aee1980f4
http://cmuir.cmu.ac.th/handle/6653943832/933
_version_ 1681419599479832576

Similar Items