Decorin modulates collagen matrix assembly and mineralization.

Decorin (DCN) is one of the major matrix proteoglycans in bone. To investigate the role of DCN in matrix mineralization, the expression of DCN in MC3T3-E1 (MC) cell cultures and the phenotypes of MC-derived clones expressing higher (sense; S-DCN) or lower (antisense; AS-DCN) levels of DCN were cha...

Full description

Saved in:
Bibliographic Details
Main Authors: Suchaya Pornprasertsuk-Damrongsri, Yoshiyuki Mochida, Duenpim Parisuthiman, Phimon Atsawasuwan, Marnisa Sricholpech, Adele L. Boskey, Mitsuo Yamauchi, สุชยา ดำรงค์ศรี
Format: Article
Language:English
Published: 2011
Subjects:
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/1083
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Mahidol University
Language: English
id th-mahidol.1083
record_format dspace
spelling th-mahidol.10832023-04-12T15:26:02Z Decorin modulates collagen matrix assembly and mineralization. Suchaya Pornprasertsuk-Damrongsri Yoshiyuki Mochida Duenpim Parisuthiman Phimon Atsawasuwan Marnisa Sricholpech Adele L. Boskey Mitsuo Yamauchi สุชยา ดำรงค์ศรี Decorin Collagen fibrillogenesis Collagen organization Matrix mineralization MC3T3-E1 cells Maturation Open Access article Decorin (DCN) is one of the major matrix proteoglycans in bone. To investigate the role of DCN in matrix mineralization, the expression of DCN in MC3T3-E1 (MC) cell cultures and the phenotypes of MC-derived clones expressing higher (sense; S-DCN) or lower (antisense; AS-DCN) levels of DCN were characterized. DCN expression was significantly decreased as the mineralized nodules were formed and expanded in vitro. In S-DCN clones, in vitro matrix mineralization was inhibited, whereas in AS-DCN clones, mineralization was accelerated. At the microscopic level, collagen fibers in S-DCN clones were thinner while those of AS-DCN clones were thicker and lacked directionality compared to the controls. At the ultrastructural level, the collagen fibrils in S-DCN clones were markedly thinner, whereas those of AS-DCN clones were larger and irregular in shape. The results from Fourier transform infrared spectroscopy analysis demonstrated that in AS-DCN cultures the mineral content was greater but the crystallinity of mineral was poorer than that of the controls at early stage of mineralization. The in vivo transplantation assay demonstrated that no mineralized matrices were formed in S-DCN transplants, whereas they were readily detected in AS-DCN transplants at 3 weeks of transplantation. The areas of bone-like matrices in AS-DCN transplants were significantly greater than the controls at 3 weeks but became comparable at 5 weeks. The bone-like matrices in AS-DCN transplants exhibited woven bone-like non-lamellar structure while the lamellar bone-like structure was evident in the control transplants. These results suggest that DCN regulates matrix mineralization by modulating collagen assembly. 2011-02-03T08:02:18Z 2011-08-29T02:20:56Z 2017-01-05T06:57:13Z 2011-02-03T08:02:18Z 2011-08-29T02:20:56Z 2017-01-05T06:57:13Z 2011-02-03 2009 Article Mochida Y, Parisuthiman D, Pornprasertsuk-Damrongsri S, Atsawasuwan P, Sricholpech M, Boskey AL, et al. Decorin modulates collagen matrix assembly and mineralization. Matrix Biol. 2009 Jan; 28(1): 44–52. 10.1016/j.matbio.2008.11.003 https://repository.li.mahidol.ac.th/handle/123456789/1083 eng Mahidol University Mahidol University 1628221 bytes application/pdf application/pdf Matrix Biology 2009; 28: 44-52.
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
language English
topic Decorin
Collagen fibrillogenesis
Collagen organization
Matrix mineralization
MC3T3-E1 cells
Maturation
Open Access article
spellingShingle Decorin
Collagen fibrillogenesis
Collagen organization
Matrix mineralization
MC3T3-E1 cells
Maturation
Open Access article
Suchaya Pornprasertsuk-Damrongsri
Yoshiyuki Mochida
Duenpim Parisuthiman
Phimon Atsawasuwan
Marnisa Sricholpech
Adele L. Boskey
Mitsuo Yamauchi
สุชยา ดำรงค์ศรี
Decorin modulates collagen matrix assembly and mineralization.
description Decorin (DCN) is one of the major matrix proteoglycans in bone. To investigate the role of DCN in matrix mineralization, the expression of DCN in MC3T3-E1 (MC) cell cultures and the phenotypes of MC-derived clones expressing higher (sense; S-DCN) or lower (antisense; AS-DCN) levels of DCN were characterized. DCN expression was significantly decreased as the mineralized nodules were formed and expanded in vitro. In S-DCN clones, in vitro matrix mineralization was inhibited, whereas in AS-DCN clones, mineralization was accelerated. At the microscopic level, collagen fibers in S-DCN clones were thinner while those of AS-DCN clones were thicker and lacked directionality compared to the controls. At the ultrastructural level, the collagen fibrils in S-DCN clones were markedly thinner, whereas those of AS-DCN clones were larger and irregular in shape. The results from Fourier transform infrared spectroscopy analysis demonstrated that in AS-DCN cultures the mineral content was greater but the crystallinity of mineral was poorer than that of the controls at early stage of mineralization. The in vivo transplantation assay demonstrated that no mineralized matrices were formed in S-DCN transplants, whereas they were readily detected in AS-DCN transplants at 3 weeks of transplantation. The areas of bone-like matrices in AS-DCN transplants were significantly greater than the controls at 3 weeks but became comparable at 5 weeks. The bone-like matrices in AS-DCN transplants exhibited woven bone-like non-lamellar structure while the lamellar bone-like structure was evident in the control transplants. These results suggest that DCN regulates matrix mineralization by modulating collagen assembly.
format Article
author Suchaya Pornprasertsuk-Damrongsri
Yoshiyuki Mochida
Duenpim Parisuthiman
Phimon Atsawasuwan
Marnisa Sricholpech
Adele L. Boskey
Mitsuo Yamauchi
สุชยา ดำรงค์ศรี
author_facet Suchaya Pornprasertsuk-Damrongsri
Yoshiyuki Mochida
Duenpim Parisuthiman
Phimon Atsawasuwan
Marnisa Sricholpech
Adele L. Boskey
Mitsuo Yamauchi
สุชยา ดำรงค์ศรี
author_sort Suchaya Pornprasertsuk-Damrongsri
title Decorin modulates collagen matrix assembly and mineralization.
title_short Decorin modulates collagen matrix assembly and mineralization.
title_full Decorin modulates collagen matrix assembly and mineralization.
title_fullStr Decorin modulates collagen matrix assembly and mineralization.
title_full_unstemmed Decorin modulates collagen matrix assembly and mineralization.
title_sort decorin modulates collagen matrix assembly and mineralization.
publishDate 2011
url https://repository.li.mahidol.ac.th/handle/123456789/1083
_version_ 1781414994256068608