Effects of 2,4,3′,5′-tetrahydroxystilbene on oxidative phosphorylation by rat liver mitochondria
The effects of an antifungal compound 2,4,3′,5′-tetrahydroxystilbene (THS)† on oxidative phosphorylation by rat liver mitochondria were studied. THS at low concentrations was found to inhibit state 3 respiration (I 50 = 44 nmoles/mg protein) but had no effect on uncoupled respiration induced by 2,4-...
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th-mahidol.108402018-04-19T21:13:21Z Effects of 2,4,3′,5′-tetrahydroxystilbene on oxidative phosphorylation by rat liver mitochondria Suwanna Nimmanpisut Prakorn Chudapongse Kavi Ratanabanangkoon Mahidol University Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics The effects of an antifungal compound 2,4,3′,5′-tetrahydroxystilbene (THS)† on oxidative phosphorylation by rat liver mitochondria were studied. THS at low concentrations was found to inhibit state 3 respiration (I 50 = 44 nmoles/mg protein) but had no effect on uncoupled respiration induced by 2,4-dinitrophenol. The inhibitory effect of THS on state 3 respiration was observed with either glutamate or succinate as respiratory substrates. However when rat liver mitochondria were respiring with glutamate as substrate and in the presence of calcium chloride and inorganic phosphate, THS produced a strong inhibition of 2,4-dinitrophenol-stimulated respiration. This inhibition was over-come by adding succinate. The tetramethoxy derivative of THS had no effect on the mitochondrial reactions mentioned above suggesting that the phenolic groups are essential for the action of THS. The sites at which THS inhibits mitochondrial oxidative phosphorylation are proposed. © 1976. 2018-04-19T14:11:00Z 2018-04-19T14:11:00Z 1976-06-01 Article Biochemical Pharmacology. Vol.25, No.11 (1976), 1245-1248 10.1016/0006-2952(76)90085-X 00062952 2-s2.0-0017153510 https://repository.li.mahidol.ac.th/handle/123456789/10840 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0017153510&origin=inward |
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Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics Suwanna Nimmanpisut Prakorn Chudapongse Kavi Ratanabanangkoon Effects of 2,4,3′,5′-tetrahydroxystilbene on oxidative phosphorylation by rat liver mitochondria |
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The effects of an antifungal compound 2,4,3′,5′-tetrahydroxystilbene (THS)† on oxidative phosphorylation by rat liver mitochondria were studied. THS at low concentrations was found to inhibit state 3 respiration (I 50 = 44 nmoles/mg protein) but had no effect on uncoupled respiration induced by 2,4-dinitrophenol. The inhibitory effect of THS on state 3 respiration was observed with either glutamate or succinate as respiratory substrates. However when rat liver mitochondria were respiring with glutamate as substrate and in the presence of calcium chloride and inorganic phosphate, THS produced a strong inhibition of 2,4-dinitrophenol-stimulated respiration. This inhibition was over-come by adding succinate. The tetramethoxy derivative of THS had no effect on the mitochondrial reactions mentioned above suggesting that the phenolic groups are essential for the action of THS. The sites at which THS inhibits mitochondrial oxidative phosphorylation are proposed. © 1976. |
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Mahidol University Suwanna Nimmanpisut Prakorn Chudapongse Kavi Ratanabanangkoon |
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Suwanna Nimmanpisut Prakorn Chudapongse Kavi Ratanabanangkoon |
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Suwanna Nimmanpisut |
title |
Effects of 2,4,3′,5′-tetrahydroxystilbene on oxidative phosphorylation by rat liver mitochondria |
title_short |
Effects of 2,4,3′,5′-tetrahydroxystilbene on oxidative phosphorylation by rat liver mitochondria |
title_full |
Effects of 2,4,3′,5′-tetrahydroxystilbene on oxidative phosphorylation by rat liver mitochondria |
title_fullStr |
Effects of 2,4,3′,5′-tetrahydroxystilbene on oxidative phosphorylation by rat liver mitochondria |
title_full_unstemmed |
Effects of 2,4,3′,5′-tetrahydroxystilbene on oxidative phosphorylation by rat liver mitochondria |
title_sort |
effects of 2,4,3′,5′-tetrahydroxystilbene on oxidative phosphorylation by rat liver mitochondria |
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2018 |
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